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Article

Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α

  • Authors:
    • Shujun Wang
    • Min Yuan
    • Lijun Song
    • Xiuying Zhang
    • Qin Geng
    • Hongju Zhang
    • Xingfu Li
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Rheumatology, People's Hospital of Liaocheng, Liaocheng, Shandong 252002, P.R. China, Department of Rheumatology, Central Hospital of Zibo, Zibo, Shandong 255036, P.R. China
  • Pages: 1590-1595
    |
    Published online on: June 9, 2017
       https://doi.org/10.3892/mmr.2017.6746
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Abstract

Gene expression can be altered through RNA interference (RNAi), including microRNA (miRNA) or small interfering RNA. Alterations of miRNAs in rheumatoid arthritis (RA) have been reported, however, the components of the RNAi machinery in RA remain to be fully elucidated. The aim of the present study was to detect the levels of Dicer, Argonaute2 and Drosha, components of the RNAi machinery, in the peripheral blood mononuclear cells of patients with RAusingreverse transcription‑quantitative polymerase chain reaction, and to compare the results with disease activity and clinical features. Disease activity was assessed using the Disease Activity Score 28 (DAS28). Transfection and stimulation of cultured cells were conducted to determine the biological function of Dicer. ELISA was used to test tumor necrosis factor (TNF)‑α protein levels. It was found that the mRNA expression levels of Dicer and Drosha were upregulated in patients with RA, and that the increased level of Dicer was correlated with disease activity in patients with RA. Dicer and TNF‑α were activated in the serum of patients with RA. The activation of Dicer suppressed the production of TNF‑α. These results suggested that Dicer can balance the production of TNF‑α, and thus may serve as a regulator of the immune response in patients with RA.
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1 

Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:806–811. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Hannon GJ: RNA interference. Nature. 418:244–251. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, Lee J, Provost P, Rådmark O, Kim S and Kim VN: The nuclear RNase III Drosha initiates microRNA processing. Nature. 425:415–419. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Bernstein E, Caudy AA, Hammond SM and Hannon GJ: Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 409:363–366. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Sevignani C, Calin GA, Siracusa LD and Croce CM: Mammalian microRNAs: A small world for fine-tuning gene expression. Mamm Genome. 17:189–202. 2006. View Article : Google Scholar : PubMed/NCBI

6 

McManus MT and Sharp PA: Gene silencing in mammals by small interfering RNAs. Nat Rev Genet. 3:737–747. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G and Tuschl T: Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Mol Cell. 15:185–197. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Halder J, Kamat AA, Landen CN Jr, Han LY, Lutgendorf SK, Lin YG, Merritt WM, Jennings NB, Chavez-Reyes A, Coleman RL, et al: Focal adhesion kinase targeting using in vivo short interfering RNA delivery in neutral liposomes for ovarian carcinoma therapy. Clin Cancer Res. 12:4916–4924. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Landen CN Jr, Chavez-Reyes A, Bucana C, Schmandt R, Deavers MT, Lopez-Berestein G and Sood AK: Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery. Cancer Res. 65:6910–6918. 2005. View Article : Google Scholar : PubMed/NCBI

10 

McInnes IB and Schett G: The pathogenesis of rheumatoid arthritis. N Engl J Med. 365:2205–2219. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Huang RY, Huang QC and Burgering BM: Novel insight into the role of α-actinin-1 in rheumatoid arthritis. Discov Med. 17:75–80. 2014.PubMed/NCBI

12 

Salemi S, Biondo MI, Fiorentino C, Argento G, Paolantonio M, Di Murro C, Malagnino VA, Canzoni M, Diamanti AP and D'Amelio R: Could early rheumatoid arthritis resolve after periodontitis treatment only? Case report and review of the literature. Medicine (Baltimore). 93:e1952014. View Article : Google Scholar : PubMed/NCBI

13 

Ursini F, Russo E, Hribal M Letizia, Mauro D, Savarino F, Bruno C, Tripolino C, Rubino M, Naty S and Grembiale RD: Abatacept improves whole-body insulin sensitivity in rheumatoid arthritis: An observational study. Medicine (Baltimore). 94:e8882015. View Article : Google Scholar : PubMed/NCBI

14 

Miao CG, Yang YY, He X, Xu T, Huang C, Huang Y, Zhang L, Lv XW, Jin Y and Li J: New advances of microRNAs in the pathogenesis of rheumatoid arthritis, with a focus on the crosstalk between DNA methylation and the microRNA machinery. Cell Signal. 25:1118–1125. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Xu T, Huang C, Chen Z and Li J: MicroRNA-323-3p: A new biomarker and potential therapeutic target for rheumatoid arthritis. Rheumatol Int. 34:721–722. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Murata K, Furu M, Yoshitomi H, Ishikawa M, Shibuya H, Hashimoto M, Imura Y, Fujii T, Ito H, Mimori T and Matsuda S: Comprehensive microRNA analysis identifies miR-24 and miR-125a-5p as plasma biomarkers for rheumatoid arthritis. PLoS One. 8:e691182013. View Article : Google Scholar : PubMed/NCBI

17 

Abou-Zeid A, Saad M and Soliman E: MicroRNA 146a expression in rheumatoid arthritis: Association with tumor necrosis factor-alpha and disease activity. Genet Test Mol Biomarkers. 15:807–812. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Philippe L, Alsaleh G, Suffert G, Meyer A, Georgel P, Sibilia J, Wachsmann D and Pfeffer S: TLR2 expression is regulated by microRNA miR-19 in rheumatoid fibroblast-like synoviocytes. J Immunol. 188:454–461. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Nagata Y, Nakasa T, Mochizuki Y, Ishikawa M, Miyaki S, Shibuya H, Yamasaki K, Adachi N, Asahara H and Ochi M: Induction of apoptosis in the synovium of mice with autoantibody-mediated arthritis by the intraarticular injection of double-stranded MicroRNA-15a. Arthritis Rheum. 60:2677–2683. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Nakasa T, Nagata Y, Yamasaki K and Ochi M: A mini-review: MicroRNA in arthritis. Physiol Genomics. 43:566–570. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Feldmann M: Translating molecular insights in autoimmunity into effective therapy. Annu Rev Immunol. 27:1–27. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Tokumaru S, Suzuki M, Yamada H, Nagino M and Takahashi T: let-7 regulates Dicer expression and constitutes a negative feedback loop. Carcinogenesis. 29:2073–2077. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Lin WJ and Yeh WC: Implication of Toll-like receptor and tumor necrosis factor alpha signaling in septic shock. Shock. 24:206–209. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Waterhouse PM, Wang MB and Lough T: Gene silencing as an adaptive defence against viruses. Nature. 411:834–842. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Meister G and Tuschl T: Mechanisms of gene silencing by double-stranded RNA. Nature. 431:343–349. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, et al: Suppression of microRNA-silencing pathway by HIV-1 during virus replication. Science. 315:1579–1582. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Matskevich AA and Moelling K: Dicer is involved in protection against influenza A virus infection. J Gen Virol. 88:2627–2635. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Kobayashi T, Lu J, Cobb BS, Rodda SJ, McMahon AP, Schipani E, Merkenschlager M and Kronenberg HM: Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci USA. 105:1949–1954. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Mizoguchi F, Izu Y, Hayata T, Hemmi H, Nakashima K, Nakamura T, Kato S, Miyasaka N, Ezura Y and Noda M: Osteoclast-specific Dicer gene deficiency suppresses osteoclastic bone resorption. J Cell Biochem. 109:866–575. 2010.PubMed/NCBI

31 

Jakymiw A, Ikeda K, Fritzler MJ, Reeves WH, Satoh M and Chan EK: Autoimmune targeting of key components of RNA interference. Arthritis Res Ther. 8:R872006. View Article : Google Scholar : PubMed/NCBI

32 

Chen G and Goeddel DV: TNF-R1 signaling: A beautiful pathway. Science. 296:1634–1655. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Guan Y, Yao H, Wang J, Sun K, Cao L and Wang Y: NF-κB-DICER-miRs axis regulates TNF-α expression in responses to endotoxin stress. Int J Biol Sci. 11:1257–1268. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Yuan M, Song L, Zhang X, Geng Q, Zhang H and Li X: Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α. Mol Med Rep 16: 1590-1595, 2017.
APA
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., & Li, X. (2017). Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α. Molecular Medicine Reports, 16, 1590-1595. https://doi.org/10.3892/mmr.2017.6746
MLA
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., Li, X."Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α". Molecular Medicine Reports 16.2 (2017): 1590-1595.
Chicago
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., Li, X."Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α". Molecular Medicine Reports 16, no. 2 (2017): 1590-1595. https://doi.org/10.3892/mmr.2017.6746
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Yuan M, Song L, Zhang X, Geng Q, Zhang H and Li X: Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α. Mol Med Rep 16: 1590-1595, 2017.
APA
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., & Li, X. (2017). Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α. Molecular Medicine Reports, 16, 1590-1595. https://doi.org/10.3892/mmr.2017.6746
MLA
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., Li, X."Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α". Molecular Medicine Reports 16.2 (2017): 1590-1595.
Chicago
Wang, S., Yuan, M., Song, L., Zhang, X., Geng, Q., Zhang, H., Li, X."Expression of Dicer in rheumatoid arthritis is associated with disease activity and balances the production of TNF‑α". Molecular Medicine Reports 16, no. 2 (2017): 1590-1595. https://doi.org/10.3892/mmr.2017.6746
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