Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder

  • Authors:
    • Jianli Feng
    • Jin Gao
    • Shan Zhou
    • Yuanfeng Liu
    • Yu Zhong
    • Yong Shu
    • Ming Sen Meng
    • Jiaqiang Yan
    • Danning Sun
    • Qiang Fang
    • Daodong Sun
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Chongqing 324 Hospital of PLA, Chongqing 400020, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1516-1522
    |
    Published online on: June 9, 2017
       https://doi.org/10.3892/mmr.2017.6749
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Stem cell factor (SCF) is critical in regulating the proliferation, differentiation and function of the interstitial cells of Cajal (ICCs), which are closely associated with smooth muscle dysfunction. The present study aimed to examine the effect of SCF on ICC proliferation and detrusor contraction in rats with an underactive bladder. Sprague‑Dawley rats were divided into four groups comprising control, control+SCF, detrusor underactivity (DU), and DU+SCF groups. The ICC count was determined using immunofluorescence; serum levels of SCF were determined using an enzyme‑linked immunosorbent assay; mRNA and protein levels of c‑kit and SCF in tissues were assessed using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively. Detrusor contractility was determined using muscle strips, based on the contraction amplitude and frequency determined in each specimen. Significantly fewer ICCs were observed in the DU group, in addition to decreased expression levels of SCF and c‑kit, compared with the control group. In addition, the detrusor contraction frequency and amplitude were markedly reduced. However, the administration of SCF significantly increased the number of ICCs, and the levels of SCF and c‑kit in animals with DU, and resulted in markedly amplified detrusor contraction frequency and amplitude. Similarly, the number of ICCs and levels of SCF and c‑kit were higher in the control+SCF group, compared with the control group. Overall, these findings suggested that exogenous SCF improved the organ dysfunction caused by reduced ICC number, providing a novel approach for organ repair.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, van Kerrebroeck P, Victor A and Wein A: Standardisation Sub-committee of the International Continence Society: The standardisation of terminology of lower urinary tract function: Report from the Standardisation Sub-committee of the International Continence Society. Neurourol Urodyn. 21:167–178. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Taylor JA III and Kuchel GA: Detrusor underactivity: Clinical features and pathogenesis of an underdiagnosed geriatric condition. J Am Geriatr Soc. 54:1920–1932. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Lorincz A, Redelman D, Horvath VJ, Bardsley MR, Chen H and Ordög T: Progenitors of interstitial cells of cajal in the postnatal murine stomach. Gastroenterology. 134:1083–1093. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Horvath VJ, Vittal H, Lörincz A, Chen H, Almeida-Porada G, Redelman D and Ordög T: Reduced stem cell factor links smooth myopathy and loss of interstitial cells of cajal in murine diabetic gastroparesis. Gastroenterology. 130:759–770. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Nishimura M, Koda K, Oda K, Seike K, Shimizu K and Miyazaki M: Mesenteric transection decreases expression of interstitial cells of Cajal in an experimental model. Br J Surg. 94:483–490. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Galli SJ, Tsai M and Wershil BK: The c-kit receptor, stem cell factor, and mast cells. What each is teaching us about the others. Am J Pathol. 142:965–974. 1993.PubMed/NCBI

7 

Torihashi S, Nishi K, Tokutomi Y, Nishi T, Ward S and Sanders KM: Blockade of kit signaling induces transdifferentiation of interstitial cells of cajal to a smooth muscle phenotype. Gastroenterology. 117:140–148. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Wu JJ, Rothman TP and Gershon MD: Development of the interstitial cell of Cajal: Origin, kit dependence and neuronal and nonneuronal sources of kit ligand. J Neurosci Res. 59:384–401. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Forrest A, Huizinga JD, Wang XY, Liu LW and Parsons M: Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus. Am J Physiol Gastrointest Liver Physiol. 294:G315–G326. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Yamamoto T, Watabe K, Nakahara M, Ogiyama H, Kiyohara T, Tsutsui S, Tamura S, Shinomura Y and Hayashi N: Disturbed gastrointestinal motility and decreased interstitial cells of Cajal in diabetic db/db mice. J Gastroenterol Hepatol. 23:660–667. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Kubota M, Kanda E, Ida K, Sakakihara Y and Hayashi M: Severe gastrointestinal dysmotility in a patient with congenital myopathy: Causal relationship to decrease of interstitial cells of Cajal. Brain Dev. 27:447–450. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Thomsen L, Robinson TL, Lee JC, Farraway LA, Hughes MJ, Andrews DW and Huizinga JD: Interstitial cells of Cajal generate a rhythmic pacemaker current. Nat Med. 4:848–851. 1998. View Article : Google Scholar : PubMed/NCBI

13 

Lin L, Xu LM, Zhang W, Ge YB, Tang YR, Zhang HJ, Li XL and Chen JD: Roles of stem cell factor on the depletion of interstitial cells of Cajal in the colon of diabetic mice. Am J Physiol Gastrointest Liver Physiol. 298:G241–G247. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Lang RJ, Tonta MA, Zoltkowski BZ, Meeker WF, Wendt I and Parkington HC: Pyeloureteric peristalsis: Role of atypical smooth muscle cells and interstitial cells of Cajal-like cells as pacemakers. J Physiol. 576:695–705. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Pezzone MA, Watkins SC, Alber SM, King WE, de Groat WC, Chancellor MB and Fraser MO: Identification of c-kit-positive cells in the mouse ureter: The interstitial cells of Cajal of the urinary tract. Am J Physiol Renal Physiol. 284:F925–F929. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Yang X, Zhang Y and Hu J: The expression of Cajal cells at the obstruction site of congenital pelviureteric junction obstruction and quantitative image analysis. J Pediatr Surg. 44:2339–2342. 2009. View Article : Google Scholar : PubMed/NCBI

17 

McCloskey KD and Gurney AM: Kit positive cells in the guinea pig bladder. J Urol. 168:832–836. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Davidson RA and McCloskey KD: Morphology and localization of interstitial cells in the guinea pig bladder: Structural relationships with smooth muscle and neurons. J Urol. 173:1385–1390. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Kim SO, Song SH, Ahn KY and Kwon DD: Distribution of interstitial cells of cajal in menopausal rat urinary bladder showing detrusor overactivity. Int Neurourol J. 14:48–53. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Kubota Y, Kojima Y, Shibata Y, Imura M, Sasaki S and Kohri K: Role of KIT-positive interstitial cells of Cajal in the urinary bladder and possible therapeutic target for overactive bladder. Adv Urol. 2011:8163422011. View Article : Google Scholar : PubMed/NCBI

21 

Sutherland RS, Baskin LS, Kogan BA and Cunha G: Neuroanatomical changes in the rat bladder after bladder outlet obstruction. Br J Urol. 82:895–901. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Wang Y, Fang Q, Lu Y, Song B, Li W and Li L: Effects of mechanical stretch on interstitial cells of Cajal in guinea pig bladder. J Surg Res. 164:e213–e219. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Kim SO, Oh BS, Chang IY, Song SH, Ahn K, Hwang EC, Oh KJ, Kwon D and Park K: Distribution of interstitial cells of Cajal and expression of nitric oxide synthase after experimental bladder outlet obstruction in a rat model of bladder overactivity. Neurourol Urodyn. 30:1639–1645. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Kubota Y, Hashitani H, Shirasawa N, Kojima Y, Sasaki S, Mabuchi Y, Soji T, Suzuki H and Kohri K: Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction. Neurourol Urodyn. 27:330–340. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Nakahara M, Isozaki K, Vanderwinden JM, Shinomura Y, Kitamura Y, Hirota S and Matsuzawa Y: Dose-dependent and time-limited proliferation of cultured murine interstitial cells of Cajal in response to stem cell factor. Life Sci. 70:2367–2376. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Tong W, Jia H, Zhang L, Li C, Ridolfi TJ and Liu B: Exogenous stem cell factor improves interstitial cells of Cajal restoration after blockade of c-kit signaling pathway. Scand J Gastroenterol. 45:844–851. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng J, Gao J, Zhou S, Liu Y, Zhong Y, Shu Y, Meng M, Yan J, Sun D, Fang Q, Fang Q, et al: Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Mol Med Rep 16: 1516-1522, 2017.
APA
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y. ... Sun, D. (2017). Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Molecular Medicine Reports, 16, 1516-1522. https://doi.org/10.3892/mmr.2017.6749
MLA
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y., Meng, M., Yan, J., Sun, D., Fang, Q., Sun, D."Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder". Molecular Medicine Reports 16.2 (2017): 1516-1522.
Chicago
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y., Meng, M., Yan, J., Sun, D., Fang, Q., Sun, D."Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder". Molecular Medicine Reports 16, no. 2 (2017): 1516-1522. https://doi.org/10.3892/mmr.2017.6749
Copy and paste a formatted citation
x
Spandidos Publications style
Feng J, Gao J, Zhou S, Liu Y, Zhong Y, Shu Y, Meng M, Yan J, Sun D, Fang Q, Fang Q, et al: Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Mol Med Rep 16: 1516-1522, 2017.
APA
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y. ... Sun, D. (2017). Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Molecular Medicine Reports, 16, 1516-1522. https://doi.org/10.3892/mmr.2017.6749
MLA
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y., Meng, M., Yan, J., Sun, D., Fang, Q., Sun, D."Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder". Molecular Medicine Reports 16.2 (2017): 1516-1522.
Chicago
Feng, J., Gao, J., Zhou, S., Liu, Y., Zhong, Y., Shu, Y., Meng, M., Yan, J., Sun, D., Fang, Q., Sun, D."Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder". Molecular Medicine Reports 16, no. 2 (2017): 1516-1522. https://doi.org/10.3892/mmr.2017.6749
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team