Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation

Sekiguchi,Yuusuke; Mano,Hiroshi; Nakatani,Sachie; Shimizu,Jun; Kataoka,Aya; Ogura,Kana; Kimira,Yoshifumi; Ebata,Midori; Wada,Masahiro

doi:10.3892/mmr.2017.6752

Mangiferin positively regulates osteoblast differentiation and suppresses osteoclast differentiation

Authors:
- Yuusuke Sekiguchi
- Hiroshi Mano
- Sachie Nakatani
- Jun Shimizu
- Aya Kataoka
- Kana Ogura
- Yoshifumi Kimira
- Midori Ebata
- Masahiro Wada
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Affiliations: Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350‑0295, Japan
Published online on: June 12, 2017 https://doi.org/10.3892/mmr.2017.6752
Pages: 1328-1332
Copyright: © Sekiguchi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mangiferin is a polyphenolic compound present in Salacia reticulata. It has been reported to reduce bone destruction and inhibit osteoclastic differentiation. This study aimed to determine whether mangiferin directly affects osteoblast and osteoclast proliferation and differentiation, and gene expression in MC3T3‑E1 osteoblastic cells and osteoclast‑like cells derived from primary mouse bone marrow macrophage cells. Mangiferin induced significantly greater WST‑1 activity, indicating increased cell proliferation. Mangiferin induced significantly increased alkaline phosphatase staining, indicating greater cell differentiation. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that mangiferin significantly increased the mRNA level of runt‑related transcription factor 2 (RunX2), but did not affect RunX1 mRNA expression. Mangiferin significantly reduced the formation of tartrate‑resistant acid phosphatase‑positive multinuclear cells. RT‑PCR demonstrated that mangiferin significantly increased the mRNA level of estrogen receptor β (ERβ), but did not affect the expression of other osteoclast‑associated genes. Mangiferin may inhibit osteoclastic bone resorption by suppressing differentiation of osteoclasts and promoting expression of ERβ mRNA in mouse bone marrow macrophage cells. It also has potential to promote osteoblastic bone formation by promoting cell proliferation and inducing cell differentiation in preosteoblast MC3T3‑E1 cells via RunX2. Mangiferin may therefore be useful in improving bone disease outcomes.

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