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Article

Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus

  • Authors:
    • Chan Mao
    • Jiang Gu
    • Hai‑Guang Wang
    • Yao Fang
    • Ping Yang
    • Bin Tang
    • Na Li
    • Ting‑Ting Wang
    • Quan‑Ming Zou
    • Qian Li
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Microbiology and Immunology, Southwest Hospital and College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, P.R. China, National Engineering Research Center for Immunobiological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University, Chongqing 400038, P.R. China, Department of Core Facilities of Imaging Facility, Medical College of Zhejiang University, Hangzhou, Zhejiang 310031, P.R. China
  • Pages: 1544-1550
    |
    Published online on: June 13, 2017
       https://doi.org/10.3892/mmr.2017.6763
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Abstract

The translocated intimin receptor (Tir) is a canonical type III secretion system effector, secreted by the enterohemorrhagic Escherichia coli (E. coli). This receptor alters the regular cellular processing of host cells, to promote intracellular bacterial replication and evasion of the host immune system. Tir is translocated and integrated into the host cell plasma membrane, a process required for its pathogenic activity in these cells, however, the underlying mechanisms of how this occurs remain to be elucidated. The present study used immunofluorescence and immunoelectron microscopy to demonstrate that the Tir of enterohemorrhagic E. coli was localized to the plasma membrane and colocalized with the 58K Golgi protein of the host cells. Treatment with brefeldin A destroyed the Golgi structure, inhibited the formation of actin pedestal and blocked the localization of Tir on the host cell plasma membrane. The results of the present study suggested that Tir is translocated to the host plasma membrane in a Golgi‑dependent manner. It may mimic the activities of eukaryotic secretory proteins in order to make use of the Golgi apparatus for transportation and integration into the plasma membrane. These findings reveal a novel trafficking pathway for the translocation of bacterial secretory effectors to their specific subcellular compartments.
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1 

Galán JE: Common themes in the design and function of bacterial effectors. Cell Host Microbe. 5:571–579. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Troisfontaines P and Cornelis GR: Type III secretion: More systems than you think. Physiology (Bethesda). 20:326–339. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Geissler B: Bacterial toxin effector-membrane targeting: Outside in, then back again. Front Cell Infect Microbiol. 2:752012. View Article : Google Scholar : PubMed/NCBI

4 

Frankel G and Phillips AD: Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: Getting off the pedestal. Cell Microbiol. 10:549–556. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Campellone KG: Cytoskeleton-modulating effectors of enteropathogenic and enterohaemorrhagic Escherichia coli: Tir, EspFU and actin pedestal assembly. FEBS J. 277:2390–2402. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Campellone KG, Robbins D and Leong JM: EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. Dev Cell. 7:217–228. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Garmendia J, Phillips AD, Carlier MF, Chong Y, Schüller S, Marches O, Dahan S, Oswald E, Shaw RK, Knutton S and Frankel G: TccP is an enterohaemorrhagic Escherichia coli O157: H7 type III effector protein that couples Tir to the actin-cytoskeleton. Cell Microbiol. 6:1167–1183. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Weiss SM, Ladwein M, Schmidt D, Ehinger J, Lommel S, Städing K, Beutling U, Disanza A, Frank R, Jänsch L, et al: IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host Microbe. 5:244–258. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Brady MJ, Campellone KG, Ghildiyal M and Leong JM: Enterohaemorrhagic and enteropathogenic Escherichia coli Tir proteins trigger a common Nck-independent actin assembly pathway. Cell Microbiol. 9:2242–2253. 2007. View Article : Google Scholar : PubMed/NCBI

10 

de Groot JC, Schlüter K, Carius Y, Quedenau C, Vingadassalom D, Faix J, Weiss SM, Reichelt J, Standfuss-Gabisch C, Lesser CF, et al: Structural basis for complex formation between human IRSp53 and the translocated intimin receptor Tir of enterohemorrhagic E. coli. Structure. 19:1294–1306. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Garmendia J, Carlier MF, Egile C, Didry D and Frankel G: Characterization of TccP-mediated N-WASP activation during enterohaemorrhagic Escherichia coli infection. Cell Microbiol. 8:1444–1455. 2006. View Article : Google Scholar : PubMed/NCBI

12 

DeVinney R, Stein M, Reinscheid D, Abe A, Ruschkowski S and Finlay BB: Enterohemorrhagic Escherichia coli O157: H7 produces Tir, which is translocated to the host cell membrane but is not tyrosine phosphorylated. Infect Immun. 67:2389–2398. 1999.PubMed/NCBI

13 

Yu S, Gu J, Wang HG, Wang QX, Luo P, Wu C, Zhang WJ, Guo G, Tong WD, Zou QM and Mao XH: Identification of a novel linear epitope on EspA from enterohemorrhagic E. coli using a neutralizing and protective monoclonal antibody. Clin Immunol. 138:77–84. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Datsenko KA and Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 97:6640–6645. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Wang H, Gu J, Yu S, Zhang W, Zhu Y, Zou Q, Zhu F and Mao X: Characterization of enterohemorrhagic Escherichia coli O157:H7 00B015: A Shiga toxin producing but virulence-attenuated isolate. Can J Microbiol. 56:651–656. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Fujiwara T, Oda K, Yokota S, Takatsuki A and Ikehara Y: Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem. 263:18545–18552. 1988.PubMed/NCBI

17 

Gruenheid S, Sekirov I, Thomas NA, Deng W, O'Donnell P, Goode D, Li Y, Frey EA, Brown NF, Metalnikov P, et al: Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol. 51:1233–1249. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Campellone KG, Brady MJ, Alamares JG, Rowe DC, Skehan BM, Tipper DJ and Leong JM: Enterohaemorrhagic Escherichia coli Tir requires a C-terminal 12-residue peptide to initiate EspF-mediated actin assembly and harbours N-terminal sequences that influence pedestal length. Cell Microbiol. 8:1488–1503. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Kenny B: The enterohaemorrhagic Escherichia coli (serotype O157:H7) Tir molecule is not functionally interchangeable for its enteropathogenic E. coli (serotype O127:H6) homologue. Cell Microbiol. 3:499–510. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Reinicke AT, Hutchinson JL, Magee AI, Mastroeni P, Trowsdale J and Kelly AP: A Salmonella typhimurium effector protein SifA is modified by host cell prenylation and S-acylation machinery. J Biol Chem. 280:14620–14627. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Kazmierczak BI and Engel JN: Pseudomonas aeruginosa ExoT acts in vivo as a GTPase-activating protein for RhoA, Rac1, and Cdc42. Infect Immun. 70:2198–2205. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Black DS and Bliska JB: The RhoGAP activity of the Yersinia pseudotuberculosis cytotoxin YopE is required for antiphagocytic function and virulence. Mol Microbiol. 37:515–527. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Kenny B, DeVinney R, Stein M, Reinscheid DJ, Frey EA and Finlay BB: Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell. 91:511–520. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Michgehl S, Heusipp G, Greune L, Rüter C and Schmidt MA: Esp-independent functional integration of the translocated intimin receptor (Tir) of enteropathogenic Escherichia coli (EPEC) into host cell membranes. Cell Microbiol. 8:625–633. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Klausner RD, Donaldson JG and Lippincott-Schwartz J: Brefeldin A: Insights into the control of membrane traffic and organelle structure. J Cell Biol. 116:1071–1080. 1992. View Article : Google Scholar : PubMed/NCBI

26 

Donaldson JG, Finazzi D and Klausner RD: Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein. Nature. 360:350–352. 1992. View Article : Google Scholar : PubMed/NCBI

27 

Kagan JC and Roy CR: Legionella phagosomes intercept vesicular traffic from endoplasmic reticulum exit sites. Nat Cell Biol. 4:945–954. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mao C, Gu J, Wang HG, Fang Y, Yang P, Tang B, Li N, Wang TT, Zou QM, Li Q, Li Q, et al: Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus. Mol Med Rep 16: 1544-1550, 2017.
APA
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B. ... Li, Q. (2017). Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus. Molecular Medicine Reports, 16, 1544-1550. https://doi.org/10.3892/mmr.2017.6763
MLA
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B., Li, N., Wang, T., Zou, Q., Li, Q."Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus". Molecular Medicine Reports 16.2 (2017): 1544-1550.
Chicago
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B., Li, N., Wang, T., Zou, Q., Li, Q."Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus". Molecular Medicine Reports 16, no. 2 (2017): 1544-1550. https://doi.org/10.3892/mmr.2017.6763
Copy and paste a formatted citation
x
Spandidos Publications style
Mao C, Gu J, Wang HG, Fang Y, Yang P, Tang B, Li N, Wang TT, Zou QM, Li Q, Li Q, et al: Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus. Mol Med Rep 16: 1544-1550, 2017.
APA
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B. ... Li, Q. (2017). Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus. Molecular Medicine Reports, 16, 1544-1550. https://doi.org/10.3892/mmr.2017.6763
MLA
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B., Li, N., Wang, T., Zou, Q., Li, Q."Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus". Molecular Medicine Reports 16.2 (2017): 1544-1550.
Chicago
Mao, C., Gu, J., Wang, H., Fang, Y., Yang, P., Tang, B., Li, N., Wang, T., Zou, Q., Li, Q."Translocation of enterohemorrhagic Escherichia coli effector Tir to the plasma membrane via host Golgi apparatus". Molecular Medicine Reports 16, no. 2 (2017): 1544-1550. https://doi.org/10.3892/mmr.2017.6763
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