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Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis

  • Authors:
    • Yu Zhang
    • Xinqian Li
    • Shengjian Fang
    • Zhenghua Zhu
    • Min Yao
    • Liyun Ying
    • Liwei Zhu
    • Zhaoxin Ma
    • Weihua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1793-1800
    |
    Published online on: June 19, 2017
       https://doi.org/10.3892/mmr.2017.6802
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Abstract

Peroxisome proliferator-activated receptor gamma (PPAR γ), is important in the immunoregulation of the allergic response. Mast cells are the most important inflammatory cells in immediate hypersensitivity and allergic diseases. However, there is limited information regarding the effects of PPAR γ on mast cell maturation. In the present study, mouse bone marrow‑derived mast cells (BMMCs) were cultured in interleukin (IL)‑3 and stem cell factor (SCF), in the presence or absence of the PPAR γ agonist, pioglitazone (PIO). The expression levels of the tyrosine kinase receptor CD117 and the high affinity IgE receptor FcεRI α, were assessed by flow cytometry, cell viability was assessed by Alamar‑Blue assay and histamine release was determined by measuring the activity of β‑hexosaminidase. IL‑3 and SCF are required for the development of mast cells in vitro. PIO dose‑dependently inhibited the expression of CD117 and FcεRI α, and the maturation of BMMCs. Treatment with PIO additionally inhibited the formation of granules and reduced the expression of β‑hexosaminidase. In addition, reverse transcription‑polymerase chain reaction analysis revealed that BMMCs treated with PIO expressed a lower level of mast cell protease (MCP)‑6 mRNA and PIO treatment enhanced the level of PPAR γ mRNA. Furthermore, PIO induced mast cell progenitor apoptosis. PPAR γ agonists may maintain mast cell homeostasis by inhibiting maturation of their precursors. The inhibitory effects of PPAR γ agonists include suppression of the activation of mast cells and a decrease in mast cell function in the inflammatory response. Therefore, PPAR γ agonists may serve as effective anti-inflammatory reagents in the treatment of allergic reactions.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Li X, Fang S, Zhu Z, Yao M, Ying L, Zhu L, Ma Z and Wang W: Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis. Mol Med Rep 16: 1793-1800, 2017.
APA
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L. ... Wang, W. (2017). Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis. Molecular Medicine Reports, 16, 1793-1800. https://doi.org/10.3892/mmr.2017.6802
MLA
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L., Zhu, L., Ma, Z., Wang, W."Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis". Molecular Medicine Reports 16.2 (2017): 1793-1800.
Chicago
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L., Zhu, L., Ma, Z., Wang, W."Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis". Molecular Medicine Reports 16, no. 2 (2017): 1793-1800. https://doi.org/10.3892/mmr.2017.6802
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Li X, Fang S, Zhu Z, Yao M, Ying L, Zhu L, Ma Z and Wang W: Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis. Mol Med Rep 16: 1793-1800, 2017.
APA
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L. ... Wang, W. (2017). Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis. Molecular Medicine Reports, 16, 1793-1800. https://doi.org/10.3892/mmr.2017.6802
MLA
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L., Zhu, L., Ma, Z., Wang, W."Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis". Molecular Medicine Reports 16.2 (2017): 1793-1800.
Chicago
Zhang, Y., Li, X., Fang, S., Zhu, Z., Yao, M., Ying, L., Zhu, L., Ma, Z., Wang, W."Peroxisome proliferator-activated receptor γ agonist suppresses mast cell maturation and induces apoptosis". Molecular Medicine Reports 16, no. 2 (2017): 1793-1800. https://doi.org/10.3892/mmr.2017.6802
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