Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line

  • Authors:
    • Xinlong Ma
    • Feng Shang
    • Qiuxia Zhang
    • Qingtang Lin
    • Shuo Han
    • Yongzhi Shan
    • Jianxin Du
    • Feng Ling
    • Hongqi Zhang
    • Geng Xu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China, Human Anatomy Division, Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
  • Pages: 2199-2204
    |
    Published online on: June 20, 2017
       https://doi.org/10.3892/mmr.2017.6809
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Aluminum-maltolate (Al‑Malt) is a potent apoptosis inductor, which has been widely reported as an etiologic factor in Alzheimer's disease (AD). MicroRNA-322 (miR‑322) is a vital regulator in various biological processes. The aim of the current study was to identify the role and possible underlying mechanism of miR‑322 in Al‑Malt‑induced apoptosis. Eight concentrations of Al‑Malt were prepared and used for treating the human neuroblastoma cell line, SH‑SY5Y. Subsequent to treatment with Al‑Malt for 3 days, cell viability, apoptosis and the expression levels of apoptosis‑associated factors were measured. In addition, the mRNA expression level of miR‑322 was monitored. Furthermore, cells were transfected with an miR‑322 mimic and/or treated with Al‑Malt, and cell viability, apoptosis and the expression levels of apoptosis‑associated factors were measured again. Al‑Malt significantly inhibited cell viability, but promoted apoptosis. The apoptosis‑associated factors, V‑Myc avian myelocytomatosis viral oncogene homolog (c‑Myc), Bcl-2-associated X protein, caspase‑3 and cleaved caspase‑3 were markedly upregulated by Al‑Malt. The mRNA expression level of miR‑322 was negatively regulated by Al‑Malt. Furthermore, miR‑322 attenuated the apoptosis induced by Al‑Malt and recovered the expression changes of these four factors. Thus, miR‑322 may attenuate Al‑Malt‑induced apoptosis by recovering the expression change of c‑Myc. Furthermore, miR‑322 may be involved in the pathogenesis of Al‑Malt‑associated AD.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Chopra K, Misra S and Kuhad A: Neurobiological aspects of Alzheimer's disease. Expert Opin Ther Targets. 15:535–555. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Sung HY, Choi BO, Jeong JH, Kong KA, Hwang J and Ahn JH: Amyloid beta-mediated hypomethylation of heme oxygenase 1 correlates with cognitive impairment in Alzheimer's disease. PLoS One. 11:e01531562016. View Article : Google Scholar : PubMed/NCBI

3 

Puthiyedth N, Riveros C, Berretta R and Moscato P: Identification of differentially expressed genes through integrated study of Alzheimer's disease affected brain regions. PLoS One. 11:e01523422016. View Article : Google Scholar : PubMed/NCBI

4 

Scheltens P, Blennow K, Breteler MM, de Strooper B, Frisoni GB, Salloway S and Van der Flier WM: Alzheimer's disease. Lancet. 388:505–517. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Zheng C, Zhou XW and Wang JZ: The dual roles of cytokines in Alzheimer's disease: update on interleukins, TNF-α, TGF-β and IFN-γ. Transl Neurodegener. 5:72016. View Article : Google Scholar : PubMed/NCBI

6 

Lévesque L, Mizzen CA, McLachlan DR and Fraser PE: Ligand specific effects on aluminum incorporation and toxicity in neurons and astrocytes. Brain Res. 877:191–202. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Wills MR and Savory J: Aluminium poisoning: Dialysis encephalopathy, osteomalacia, and anaemia. Lancet. 2:29–34. 1983. View Article : Google Scholar : PubMed/NCBI

8 

Garruto RM: Pacific paradigms of environmentally-induced neurological disorders: Clinical, epidemiological and molecular perspectives. Neurotoxicology. 12:347–377. 1991.PubMed/NCBI

9 

Khachaturian ZS: Diagnosis of Alzheimer's disease. Arch Neurol. 42:1097–1105. 1985. View Article : Google Scholar : PubMed/NCBI

10 

Perl DP and Brody AR: Alzheimer's disease: X-ray spectrometric evidence of aluminum accumulation in neurofibrillary tangle-bearing neurons. Science. 208:297–299. 1980. View Article : Google Scholar : PubMed/NCBI

11 

Sánchez-Iglesias S, Méndez-Alvarez E, Iglesias-González J, Muñoz-Patiño A, Sánchez-Sellero I, Labandeira-García JL and Soto-Otero R: Brain oxidative stress and selective behaviour of aluminium in specific areas of rat brain: Potential effects in a 6-OHDA-induced model of Parkinson's disease. J Neurochem. 109:879–888. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Johnson VJ, Kim SH and Sharma RP: Aluminum-maltolate induces apoptosis and necrosis in neuro-2a cells: Potential role for p53 signaling. Toxicol Sci. 83:329–339. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Gralla EJ, Stebbins RB, Coleman GL and Delahunt CS: Toxicity studies with ethyl maltol. Toxicol Appl Pharmacol. 15:604–613. 1969. View Article : Google Scholar : PubMed/NCBI

14 

Zhu M, Huang C, Ma X, Wu R, Zhu W, Li X, Liang Z, Deng F, Zhu J, Xie W, et al: Modulation of miR-19 in aluminum-induced neural cell apoptosis. J Alzheimers Dis. 50:1149–1162. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Qin W, Xie W, Yang X, Xia N and Yang K: Inhibiting microRNA-449 attenuates cisplatin-induced injury in NRK-52E cells possibly via regulating the SIRT1/P53/BAX pathway. Med Sci Monit. 22:818–823. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Ardekani AM and Naeini MM: The role of microRNAs in human diseases. Avicenna J Med Biotechnol. 2:161–179. 2010.PubMed/NCBI

17 

Lukiw WJ: Micro-RNA speciation in fetal, adult and Alzheimer's disease hippocampus. Neuroreport. 18:297–300. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Zhang C, Lu J, Liu B, Cui Q and Wang Y: Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease. Aging (Albany NY). 8:272–290. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Absalon S, Kochanek DM, Raghavan V and Krichevsky AM: MiR-26b, upregulated in Alzheimer's disease, activates cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons. J Neurosci. 33:14645–14659. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Zhang B, Chen CF, Wang AH and Lin QF: MiR-16 regulates cell death in Alzheimer's disease by targeting amyloid precursor protein. Eur Rev Med Pharmacol Sci. 19:4020–4027. 2015.PubMed/NCBI

21 

Modi PK, Jaiswal S and Sharma P: Regulation of neuronal cell cycle and apoptosis by MicroRNA 34a. Mol Cell Biol. 36:84–94. 2015.PubMed/NCBI

22 

Bertholf RL, Herman MM, Savory J, Carpenter RM, Sturgill BC, Katsetos CD, Vandenberg SR and Wills MR: A long-term intravenous model of aluminum maltol toxicity in rabbits: Tissue distribution, hepatic, renal, and neuronal cytoskeletal changes associated with systemic exposure. Toxicol Appl Pharmacol. 98:58–74. 1989. View Article : Google Scholar : PubMed/NCBI

23 

Lu L, Li C, Li D, Wang Y, Zhou C, Shao W, Peng J, You Y, Zhang X and Shen X: Cryptotanshinone inhibits human glioma cell proliferation by suppressing STAT3 signaling. Mol Cell Biochem. 381:273–282. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Savory J, Rao JK, Huang Y, Letada PR and Herman MM: Age-related hippocampal changes in Bcl-2: Bax ratio, oxidative stress, redox-active iron and apoptosis associated with aluminum-induced neurodegenerati: Increased susceptibility with aging. Neurotoxicology. 20:805–817. 1999.PubMed/NCBI

26 

Hoffman B and Liebermann DA: Apoptotic signaling by c-MYC. Oncogene. 27:6462–6472. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Askew DS, Ashmun RA, Simmons BC and Cleveland JL: Constitutive c-myc expression in an IL-3-dependent myeloid cell line suppresses cell cycle arrest and accelerates apoptosis. Oncogene. 6:1915–1922. 1991.PubMed/NCBI

28 

Evan GI, Wyllie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, Waters CM, Penn LZ and Hancock DC: Induction of apoptosis in fibroblasts by c-myc protein. Cell. 69:119–128. 1992. View Article : Google Scholar : PubMed/NCBI

29 

Dansen TB, Whitfield J, Rostker F, Brown-Swigart L and Evan GI: Specific requirement for Bax, not Bak, in Myc-induced apoptosis and tumor suppression in vivo. J Biol Chem. 281:10890–10895. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Cao S, Xiao L, Rao JN, Zou T, Liu L, Zhang D, Turner DJ, Gorospe M and Wang JY: Inhibition of Smurf2 translation by miR-322/503 modulates TGF-β/Smad2 signaling and intestinal epithelial homeostasis. Mol Biol Cell. 25:1234–1243. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Gámez B, Rodríguez-Carballo E, Bartrons R, Rosa JL and Ventura F: MicroRNA-322 (miR-322) and its target protein Tob2 modulate osterix (Osx) mRNA stability. J Biol Chem. 288:14264–14275. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Merlet E, Atassi F, Motiani RK, Mougenot N, Jacquet A, Nadaud S, Capiod T, Trebak M, Lompré AM and Marchand A: miR-424/322 regulates vascular smooth muscle cell phenotype and neointimal formation in the rat. Cardiovasc Res. 98:458–468. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Gu H, Yu J, Dong D, Zhou Q, Wang JY and Yang P: The miR-322-TRAF3 circuit mediates the pro-apoptotic effect of high glucose on neural stem cells. Toxicol Sci. 144:186–196. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ma X, Shang F, Zhang Q, Lin Q, Han S, Shan Y, Du J, Ling F, Zhang H, Xu G, Xu G, et al: MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line. Mol Med Rep 16: 2199-2204, 2017.
APA
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y. ... Xu, G. (2017). MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line. Molecular Medicine Reports, 16, 2199-2204. https://doi.org/10.3892/mmr.2017.6809
MLA
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y., Du, J., Ling, F., Zhang, H., Xu, G."MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line". Molecular Medicine Reports 16.2 (2017): 2199-2204.
Chicago
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y., Du, J., Ling, F., Zhang, H., Xu, G."MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line". Molecular Medicine Reports 16, no. 2 (2017): 2199-2204. https://doi.org/10.3892/mmr.2017.6809
Copy and paste a formatted citation
x
Spandidos Publications style
Ma X, Shang F, Zhang Q, Lin Q, Han S, Shan Y, Du J, Ling F, Zhang H, Xu G, Xu G, et al: MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line. Mol Med Rep 16: 2199-2204, 2017.
APA
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y. ... Xu, G. (2017). MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line. Molecular Medicine Reports, 16, 2199-2204. https://doi.org/10.3892/mmr.2017.6809
MLA
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y., Du, J., Ling, F., Zhang, H., Xu, G."MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line". Molecular Medicine Reports 16.2 (2017): 2199-2204.
Chicago
Ma, X., Shang, F., Zhang, Q., Lin, Q., Han, S., Shan, Y., Du, J., Ling, F., Zhang, H., Xu, G."MicroRNA-322 attenuates aluminum maltolate-induced apoptosis in the human SH-SY5Y neuroblastoma cell line". Molecular Medicine Reports 16, no. 2 (2017): 2199-2204. https://doi.org/10.3892/mmr.2017.6809
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team