Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells

  • Authors:
    • Xuefeng Yang
    • Handong Huang
    • Maijian Wang
    • Xingbin Zheng
    • Jie Xu
    • Ming Xie
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, School of Public Health, Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China
  • Pages: 2211-2216
    |
    Published online on: June 20, 2017
       https://doi.org/10.3892/mmr.2017.6817
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Nonylphenol (NP) is a well-known endocrine-disrupting chemical (EDC), which can enhance the progression of cancer by functioning as an estrogen‑like factor. In the present study, the effects of different concentrations of NP on COLO205 colorectal cancer (CRC) cells were examined. The results of flow cytometric analysis revealed that NP significantly decreased the proportion of cells in the G0/G1 phase in a dose‑dependent manner, which was accompanied by a marginal increase in the proportions of cells in S and G2/M phases. NP did not induce apoptosis, whereas estradiol (E2) did induce apoptosis. To elucidate the mechanisms underlying the action of NP on COLO205 cells, the transcriptional levels of extracellular signal‑regulated kinase (ERK)1, ERK2 and phosphoinositide 3‑kinase (PI3K) were assessed using reverse transcription‑quantitative polymerase chain reaction analysis. The expressions levels of ERK1, ERK2 and PI3K were increased by treatment with NP in a dose‑dependent manner. On examining protein levels, the expression of PI3K p38 was increased by NP and E2, and the expression of ERK1/2 was increased by NP. The phosphorylation of the ERK protein was significantly increased by treatment with NP at a high concentration (10‑4 M; P<0.01), but significantly decreased by E2 (P<0.01). Two key proteins in the transforming growth factor (TGF)β pathway (c‑Fos and SnoN) were selected for analysis using western blot analysis in the COLO205 cells treated with NP and E2. The expression levels of c‑Fos and SnoN were significantly increased by treatment with E2 (10‑7 M; P<0.01) and NP (10‑7‑10‑4 M; P<0.01). Taken together, these results indicated that NP affected the development of CRC via the ERK signaling pathway and TGFβ pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Diamanti-Kandarakis E, Bourguignon JP, Giudice LC, Hauser R, Prins GS, Soto AM, Zoeller RT and Gore AC: Endocrine-disrupting chemicals: An endocrine society scientific statement. Endocr Rev. 30:293–342. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Diamanti-Kandarakis E, Palioura E, Kandarakis SA and Koutsilieris M: The impact of endocrine disruptors on endocrine targets. Horm Metab Res. 42:543–552. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Yoon K, Kwack SJ, Kim HS and Lee BM: Estrogenic endocrine-disrupting chemicals: Molecular mechanisms of actions on putative human diseases. J Toxicol Environ Health B Crit Rev. 17:127–174. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Safe SH, Pallaroni L, Yoon K, Gaido K, Ross S, Saville B and McDonnellc D: Toxicology of environmental estrogens. Reprod Fertil Dev. 13:307–315. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Choi SM, Yoo SD and Lee BM: Toxicological characteristics of endocrine-disrupting chemicals: Developmental toxicity, carcinogenicity, and mutagenicity. J Toxicol Environ Health B Crit Rev. 7:1–24. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Bonefeld-Jørgensen EC, Long M, Hofmeister MV and Vinggaard AM: Endocrine-disrupting potential of bisphenol A, bisphenol A dimethacrylate, 4-n-nonylphenol, and 4-n-octylphenol in vitro: New data and a brief review. Environ Health Perspect. 115 Suppl 1:S69–S76. 2007. View Article : Google Scholar

7 

Kim YS, Hwang KA, Hyun SH, Nam KH, Lee CK and Choi KC: Bisphenol A and nonylphenol have the potential to stimulate the migration of ovarian cancer cells by inducing epithelial-mesenchymal transition via an estrogen receptor dependent pathway. Chem Res Toxicol. 28:662–671. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Park MA and Choi KC: Effects of 4-nonylphenol and bisphenol A on stimulation of cell growth via disruption of the transforming growth factor-β signaling pathway in ovarian cancer models. Chem Res Toxicol. 27:119–128. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Kim SH, Nam KH, Hwang KA and Choi KC: Influence of hexabromocyclododecane and 4-nonylphenol on the regulation of cell growth, apoptosis and migration in prostatic cancer cells. Toxicol In Vitro. 32:240–247. 2016. View Article : Google Scholar : PubMed/NCBI

10 

In SJ, Kim SH, Go RE, Hwang KA and Choi KC: Benzophenone-1 and nonylphenol stimulated MCF-7 breast cancer growth by regulating cell cycle and metastasis-related genes via an estrogen receptor α-dependent pathway. J Toxicol Environ Health A. 78:492–505. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Van Cutsem E and Oliveira J; ESMO Guidelines Working Group, : Advanced colorectal cancer: ESMO clinical recommendations for diagnosis, treatment and follow-up. Ann Oncol. 20 Suppl 4:S61–S63. 2009. View Article : Google Scholar

13 

Kennelly R, Kavanagh DO, Hogan AM and Winter DC: Oestrogen and the colon: Potential mechanisms for cancer prevention. Lancet Oncol. 9:385–391. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Lin JH and Giovannucci E: Sex hormones and colorectal cancer: What have we learned so far? J Natl Cancer Inst. 102:1746–1747. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Grodstein F, Newcomb PA and Stampfer MJ: Postmenopausal hormone therapy and the risk of colorectal cancer: A review and meta-analysis. Am J Med. 106:574–582. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Gunter MJ, Hoover DR, Yu H, Wassertheil-Smoller S, Rohan TE, Manson JE, Howard BV, Wylie-Rosett J, Anderson GL, Ho GY, et al: Insulin, insulin-like growth factor-I, endogenous estradiol and risk of colorectal cancer in postmenopausal women. Cancer Res. 68:329–337. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Clendenen TV, Koenig KL, Shore RE, Levitz M, Arslan AA and Zeleniuch-Jacquotte A: Postmenopausal levels of endogenous sex hormones and risk of colorectal cancer. Cancer Epidemiol Biomarkers Prev. 18:275–281. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Wu H, Xu L, Chen J, Hu J, Yu S, Hu G, Huang L, Chen X, Yuan X and Li G: Association of estrogen receptor beta variants and serum levels of estradiol with risk of colorectal cancer: A case control study. BMC Cancer. 12:2762012. View Article : Google Scholar : PubMed/NCBI

19 

Sato R, Suzuki T, Katayose Y, Miura K, Shiiba K, Tateno H, Miki Y, Akahira J, Kamogawa Y, Nagasaki S, Yamamoto K, et al: Steroid sulfatase and estrogen sulfotransferase in colon carcinoma: Regulators of intratumoral estrogen concentrations and potent prognostic factors. Cancer Res. 69:914–922. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-(Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Yang O, Kim HL, Weon JI and Seo YR: Endocrine-disrupting chemicals: Review of toxicological mechanisms using molecular pathway analysis. J Cancer Prev. 20:12–24. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Ariemma F, D'Esposito V, Liguoro D, Oriente F, Cabaro S, Liotti A, Cimmino I, Longo M, Beguinot F, Formisano P and Valentino R: Low-dose bisphenol-A impairs adipogenesis and generates dysfunctional 3T3-L1 adipocytes. PloS One. 11:e01507622016. View Article : Google Scholar : PubMed/NCBI

23 

Jambor T, Lukáčová J, Tvrdá E, Knážická Z, Forgács Z and Lukáč N: The impact of 4-nonylphenol on the viability and hormone production of mouse leydig cells. Folia Biol (Praha). 62:34–39. 2016.PubMed/NCBI

24 

Lee HR, Hwang KA, Park MA, Yi BR, Jeung EB and Choi KC: Treatment with bisphenol A and methoxychlor results in the growth of human breast cancer cells and alteration of the expression of cell cycle-related genes, cyclin D1 and p21, via an estrogen receptor-dependent signaling pathway. Int J Mol Med. 29:883–890. 2012.PubMed/NCBI

25 

Dong Y, Araki M, Hirane M, Tanabe E, Fukushima N and Tsujiuchi T: Effects of bisphenol A and 4-nonylphenol on cellular responses through the different induction of LPA receptors in liver epithelial WB-F344 cells. J Recept Signal Transduct Res. 34:201–204. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Wei Z, Ma W, Qi X, Zhu X, Wang Y, Xu Z, Luo J, Wang D, Guo W, Li X, et al: Pinin facilitated proliferation and metastasis of colorectal cancer through activating EGFR/ERK signaling pathway. Oncotarget. 7:29429–29439. 2016.PubMed/NCBI

27 

Jeng YJ and Watson CS: Combinations of physiologic estrogens with xenoestrogens alter ERK phosphorylation profiles in rat pituitary cells. Environ Health Perspect. 119:104–112. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Watson CS, Jeng YJ, Hu G, Wozniak A, Bulayeva N and Guptarak J: Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I. Steroids. 77:424–432. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Derynck R, Akhurst RJ and Balmain A: TGF-beta signaling in tumor suppression and cancer progression. Nat Genet. 29:117–129. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Milde-Langosch K: The Fos family of transcription factors and their role in tumourigenesis. Eur J Cancer. 41:2449–2461. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Deheuninck J and Luo K: Ski and SnoN, potent negative regulators of TGF-beta signaling. Cell Res. 19:47–57. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang X, Huang H, Wang M, Zheng X, Xu J and Xie M: Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells. Mol Med Rep 16: 2211-2216, 2017.
APA
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., & Xie, M. (2017). Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells. Molecular Medicine Reports, 16, 2211-2216. https://doi.org/10.3892/mmr.2017.6817
MLA
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., Xie, M."Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells". Molecular Medicine Reports 16.2 (2017): 2211-2216.
Chicago
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., Xie, M."Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells". Molecular Medicine Reports 16, no. 2 (2017): 2211-2216. https://doi.org/10.3892/mmr.2017.6817
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Huang H, Wang M, Zheng X, Xu J and Xie M: Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells. Mol Med Rep 16: 2211-2216, 2017.
APA
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., & Xie, M. (2017). Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells. Molecular Medicine Reports, 16, 2211-2216. https://doi.org/10.3892/mmr.2017.6817
MLA
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., Xie, M."Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells". Molecular Medicine Reports 16.2 (2017): 2211-2216.
Chicago
Yang, X., Huang, H., Wang, M., Zheng, X., Xu, J., Xie, M."Effect of nonylphenol on the regulation of cell growth in colorectal cancer cells". Molecular Medicine Reports 16, no. 2 (2017): 2211-2216. https://doi.org/10.3892/mmr.2017.6817
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team