Open Access

Proteomics of acute heart failure in a rat post-myocardial infarction model

  • Authors:
    • Yichen Guo
    • Lianqun Cui
    • Shiliang Jiang
    • Airong Zhang
    • Shu Jiang
  • View Affiliations

  • Published online on: June 20, 2017     https://doi.org/10.3892/mmr.2017.6820
  • Pages: 1946-1956
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify the mechanisms underlying the development of post-myocardial infarction (post-MI) heart failure. The left anterior descending coronary artery of rats was occluded to mimic human ischemic heart disease. Linear Trap Quadropole OrbiTrap mass spectrometry was used to profile the expressions of energy metabolism‑associated and calcium‑binding proteins in the post‑MI and control groups. Using the online Protein Analysis Through Evolutionary Relationships classification system, 78 differentially expressed proteins were identified, including 50 downregulated proteins and 28 upregulated proteins in post‑MI group when compared with the control group. The differentially expressed proteins were closely associated with energy metabolism, contractile function, calcium handling, pathological hypertrophy and cardiac remodeling. These results were further validated using western blotting. At different postoperative time points (1st and 14th day following surgery) during the progression of advanced heart failure post‑MI, dynamic alterations in differential protein expression were identified. The expression of the vitamin D protein was significantly upregulated on the 1st day post‑MI however, was then downregulated with progression of the disease on the 14th day post‑MI. These results identified various target proteins associated with the disease, which may be used as diagnostic markers.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Guo Y, Cui L, Jiang S, Zhang A and Jiang S: Proteomics of acute heart failure in a rat post-myocardial infarction model. Mol Med Rep 16: 1946-1956, 2017
APA
Guo, Y., Cui, L., Jiang, S., Zhang, A., & Jiang, S. (2017). Proteomics of acute heart failure in a rat post-myocardial infarction model. Molecular Medicine Reports, 16, 1946-1956. https://doi.org/10.3892/mmr.2017.6820
MLA
Guo, Y., Cui, L., Jiang, S., Zhang, A., Jiang, S."Proteomics of acute heart failure in a rat post-myocardial infarction model". Molecular Medicine Reports 16.2 (2017): 1946-1956.
Chicago
Guo, Y., Cui, L., Jiang, S., Zhang, A., Jiang, S."Proteomics of acute heart failure in a rat post-myocardial infarction model". Molecular Medicine Reports 16, no. 2 (2017): 1946-1956. https://doi.org/10.3892/mmr.2017.6820