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Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line

  • Authors:
    • Hao Tao
    • Feng Chen
    • Haifei Liu
    • Yanling Hu
    • Yingzhen Wang
    • Haiyan Li
  • View Affiliations / Copyright

    Affiliations: Department of Trauma Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Joint Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1701-1706
    |
    Published online on: June 21, 2017
       https://doi.org/10.3892/mmr.2017.6828
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Abstract

Anaberrant Wnt/β-catenin signaling pathway is frequently implicated in tumorigenesis. However, whether the Wnt/β‑catenin pathway plays a role in resistance to antitumor chemotherapy drugs remains unknown. In the present study, the process of autophagy was assessed following overexpression of the autophagy‑associated gene Beclin 1 in gemcitabine‑induced MG63 human osteosarcoma cells. Autophagy‑associated gene expression was measured following activation or inhibition of the Wnt/β‑catenin pathway in gemcitabine‑induced MG63 cells using reverse transcription‑quantitative polymerase chain reaction. In addition, the percentage of MG63 cell apoptosis was measured by flow cytometry following Wnt/β‑catenin pathway activation or inhibition. The results demonstrated that Beclin 1 overexpression induced autophagy and reduced gemcitabine‑induced apoptosis in MG63 human cell line. Furthermore, activation of the Wnt/β‑catenin signaling pathway attenuated autophagy and enhanced gemcitabine‑induced apoptosis. Additionally, the expression of Beclin 1 was reduced following Wnt/β‑catenin signaling pathway activation. The present study demonstrated that activation of the Wnt/β‑catenin signaling pathway may rescue chemotherapy drug resistance by downregulating the expression of Beclin 1.
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Copy and paste a formatted citation
Spandidos Publications style
Tao H, Chen F, Liu H, Hu Y, Wang Y and Li H: Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line. Mol Med Rep 16: 1701-1706, 2017.
APA
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., & Li, H. (2017). Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line. Molecular Medicine Reports, 16, 1701-1706. https://doi.org/10.3892/mmr.2017.6828
MLA
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., Li, H."Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line". Molecular Medicine Reports 16.2 (2017): 1701-1706.
Chicago
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., Li, H."Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line". Molecular Medicine Reports 16, no. 2 (2017): 1701-1706. https://doi.org/10.3892/mmr.2017.6828
Copy and paste a formatted citation
x
Spandidos Publications style
Tao H, Chen F, Liu H, Hu Y, Wang Y and Li H: Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line. Mol Med Rep 16: 1701-1706, 2017.
APA
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., & Li, H. (2017). Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line. Molecular Medicine Reports, 16, 1701-1706. https://doi.org/10.3892/mmr.2017.6828
MLA
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., Li, H."Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line". Molecular Medicine Reports 16.2 (2017): 1701-1706.
Chicago
Tao, H., Chen, F., Liu, H., Hu, Y., Wang, Y., Li, H."Wnt/β-catenin signaling pathway activation reverses gemcitabine resistance by attenuating Beclin1-mediated autophagy in the MG63 human osteosarcoma cell line". Molecular Medicine Reports 16, no. 2 (2017): 1701-1706. https://doi.org/10.3892/mmr.2017.6828
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