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Article

Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest

  • Authors:
    • Huijun Liu
    • Weiwei Liu
    • Xin Zhou
    • Chongde Long
    • Xielan Kuang
    • Jie Hu
    • Yan Tang
    • Lanying Liu
    • Jia He
    • Zixin Huang
    • Yuting Fan
    • Guorong Jin
    • Qingjiong Zhang
    • Huangxuan Shen
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China
  • Pages: 2069-2074
    |
    Published online on: June 21, 2017
       https://doi.org/10.3892/mmr.2017.6838
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Abstract

Oxidative damage is a key factor for the pathogenesis of age‑related macular degeneration (AMD), therefore, anti-oxidative stress is a valuable method for the prevention or treatment of AMD. The aim of the present study was to reveal the protective mechanism of lutein on retinal pigment epithelium (RPE) cells subjected to oxidative stress. Acute retinal pigment epithelial 19 (ARPE‑19) cells were exposed to oxidative stress induced by H2O2 following lutein pretreatment. The activities of caspases, level of intracellular reactive oxygen species (ROS) and cell cycle were analyzed using flow cytometry. The expression levels of cell cycle regulatory proteins and inflammation‑associated genes were detected using western blot and reverse transcription‑polymerase chain reaction analyses, respectively. The data showed that oxidative stress reduced cell viability, and increased total apoptosis and ROS generation, however, lutein prevented cells from oxidative stress‑induced damage. In addition, oxidative damage triggered G2/M phase arrest of the ARPE‑19 cells, which was reversed by lutein in a concentration‑dependent manner, through the activation of cyclin‑dependent kinase 1 and cell division cycle 25C, and degradation of cyclin B1. These results demonstrated that lutein may be an effective antioxidant, which can be applied in the prevention of AMD, or other age-related diseases associated with oxidative damage.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Liu W, Zhou X, Long C, Kuang X, Hu J, Tang Y, Liu L, He J, Huang Z, Huang Z, et al: Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Mol Med Rep 16: 2069-2074, 2017.
APA
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J. ... Shen, H. (2017). Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Molecular Medicine Reports, 16, 2069-2074. https://doi.org/10.3892/mmr.2017.6838
MLA
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J., Tang, Y., Liu, L., He, J., Huang, Z., Fan, Y., Jin, G., Zhang, Q., Shen, H."Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest". Molecular Medicine Reports 16.2 (2017): 2069-2074.
Chicago
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J., Tang, Y., Liu, L., He, J., Huang, Z., Fan, Y., Jin, G., Zhang, Q., Shen, H."Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest". Molecular Medicine Reports 16, no. 2 (2017): 2069-2074. https://doi.org/10.3892/mmr.2017.6838
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Liu W, Zhou X, Long C, Kuang X, Hu J, Tang Y, Liu L, He J, Huang Z, Huang Z, et al: Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Mol Med Rep 16: 2069-2074, 2017.
APA
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J. ... Shen, H. (2017). Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest. Molecular Medicine Reports, 16, 2069-2074. https://doi.org/10.3892/mmr.2017.6838
MLA
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J., Tang, Y., Liu, L., He, J., Huang, Z., Fan, Y., Jin, G., Zhang, Q., Shen, H."Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest". Molecular Medicine Reports 16.2 (2017): 2069-2074.
Chicago
Liu, H., Liu, W., Zhou, X., Long, C., Kuang, X., Hu, J., Tang, Y., Liu, L., He, J., Huang, Z., Fan, Y., Jin, G., Zhang, Q., Shen, H."Protective effect of lutein on ARPE-19 cells upon H2O2-induced G2/M arrest". Molecular Medicine Reports 16, no. 2 (2017): 2069-2074. https://doi.org/10.3892/mmr.2017.6838
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