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A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells

  • Authors:
    • Yuan Yao
    • Qiyue Deng
    • Chao Sun
    • Weiling Song
    • Huan Liu
    • Yue Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Neurobiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, P.R. China, Department of Ophthalmology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1991-2001
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    Published online on: June 22, 2017
       https://doi.org/10.3892/mmr.2017.6846
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Abstract

It has been hypothesized that intervertebral disc degeneration is initiated by degeneration of the cartilage endplate (CEP), which is characterized by cartilage ossification. CEP‑derived stem cells (CESCs), with the potential for chondro‑osteogenic differentiation, may be responsible for the balance between chondrification and ossification in the CEP. The CEP remains in an avascular and hypoxic microenvironment; the present study observed that hypoxia was able to markedly inhibit the osteogenic differentiation of CESCs. This tissue‑specific CESC differentiation in response to a hypoxic microenvironment was physiologically important for the prevention of ossification in the CEP. In order to study the hypoxia‑regulated mechanisms underlying osteogenic differentiation of CESCs, a Human Transcriptome Array 2.0 was used to detect differentially expressed genes (DEGs) and alternatively spliced genes (ASGs) during the osteogenic differentiation of CESCs under hypoxia, compared with those induced under normoxia. High‑throughput analysis of DEGs and ASGs demonstrated that genes in the complement pathway were enriched, which may be a potential mechanism underlying hypoxia inhibition of CESCs osteogenesis. The results of the present study may provide a basis for future mechanistic studies regarding gene expression levels and alternative splicing events during the hypoxia‑regulated inhibition of osteogenesis, which may be helpful in identifying targets for CEP degeneration therapy.
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1 

Andersson GB: Epidemiological features of chronic low-back pain. Lancet. 354:581–585. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Freemont AJ: The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. Rheumatology (Oxford). 48:5–10. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Stokes IA and Iatridis JC: Mechanical conditions that accelerate intervertebral disc degeneration: Overload versus immobilization. Spine (Phila Pa 1976). 29:2724–2732. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Le Maitre CL, Freemont AJ and Hoyland JA: Accelerated cellular senescence in degenerate intervertebral discs: A possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther. 9:R452007. View Article : Google Scholar : PubMed/NCBI

5 

Zhao CQ, Wang LM, Jiang LS and Dai LY: The cell biology of intervertebral disc aging and degeneration. Ageing Res Rev. 6:247–261. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Urban JP, Smith S and Fairbank JC: Nutrition of the intervertebral disc. Spine (Phila Pa 1976). 29:2700–2709. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Buckwalter JA: Aging and degeneration of the human intervertebral disc. Spine (Phila Pa 1976). 20:1307–1314. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Holm S, Maroudas A, Urban JP, Selstam G and Nachemson A: Nutrition of the intervertebral disc: Solute transport and metabolism. Connect Tissue Res. 8:101–119. 1981. View Article : Google Scholar : PubMed/NCBI

9 

Raj PP: Intervertebral disc: Anatomy-physiology- pathophysiology-treatment. Pain Pract. 8:18–44. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Li FC, Zhang N, Chen WS and Chen QX: Endplate degeneration may be the origination of the vacuum phenomenon in intervertebral discs. Med Hypotheses. 75:169–171. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Jackson AR, Huang CY and Gu WY: Effect of endplate calcification and mechanical deformation on the distribution of glucose in intervertebral disc: A 3D finite element study. Comput Methods Biomech Biomed Engin. 14:195–204. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Roberts S, Urban JP, Evans H and Eisenstein SM: Transport properties of the human cartilage endplate in relation to its composition and calcification. Spine (Phila Pa 1976). 21:415–420. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Liu LT, Huang B, Li CQ, Zhuang Y, Wang J and Zhou Y: Characteristics of stem cells derived from the degenerated human intervertebral disc cartilage endplate. PLoS One. 6:e262852011. View Article : Google Scholar : PubMed/NCBI

14 

Boskey AL: Signaling in response to hypoxia and normoxia in the intervertebral disc. Arthritis Rheum. 58:3637–3639. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Merceron C, Vinatier C, Portron S, Masson M, Amiaud J, Guigand L, Chérel Y, Weiss P and Guicheux J: Differential effects of hypoxia on osteochondrogenic potential of human adipose-derived stem cells. Am J Physiol Cell Physiol. 298:C355–C364. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Makino Y, Kanopka A, Wilson WJ, Tanaka H and Poellinger L: Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia-inducible factor-3alpha locus. J Biol Chem. 277:32405–32408. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Tacconelli A, Farina AR, Cappabianca L, Desantis G, Tessitore A, Vetuschi A, Sferra R, Rucci N, Argenti B, Screpanti I, et al: TrkA alternative splicing: A regulated tumor-promoting switch in human neuroblastoma. Cancer Cell. 6:347–360. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Kazantseva J, Kivil A, Tints K, Kazantseva A, Neuman T and Palm K: Alternative splicing targeting the hTAF4-TAFH domain of TAF4 represses proliferation and accelerates chondrogenic differentiation of human mesenchymal stem cells. PLoS One. 8:e747992013. View Article : Google Scholar : PubMed/NCBI

19 

Kazantseva J, Kivil A, Tints K, Kazantseva A, Neuman T and Palm K: PTHrP in differentiating human mesenchymal stem cells: Transcript isoform expression, promoter methylation, and protein accumulation. Biochimie. 95:1888–1896. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Hang X, Li P, Li Z, Qu W, Yu Y, Li H, Shen Z, Zheng H, Gao Y, Wu Y, et al: Transcription and splicing regulation in human umbilical vein endothelial cells under hypoxic stress conditions by exon array. BMC Genomics. 10:1262009. View Article : Google Scholar : PubMed/NCBI

21 

Moller-Levet CS, Betts GN, Harris AL, Homer JJ, West CM and Miller CJ: Exon array analysis of head and neck cancers identifies a hypoxia related splice variant of LAMA3 associated with a poor prognosis. PLoS Comput Biol. 5:e10005712009. View Article : Google Scholar : PubMed/NCBI

22 

Ducy P, Zhang R, Geoffroy V, Ridall AL and Karsenty G: Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell. 89:747–754. 1997. View Article : Google Scholar : PubMed/NCBI

23 

Lynch MP, Stein JL, Stein GS and Lian JB: The influence of type I collagen on the development and maintenance of the osteoblast phenotype in primary and passaged rat calvarial osteoblasts: Modification of expression of genes supporting cell growth, adhesion, and extracellular matrix mineralization. Exp Cell Res. 216:35–45. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Andrianarivo AG, Robinson JA, Mann KG and Tracy RP: Growth on type I collagen promotes expression of the osteoblastic phenotype in human osteosarcoma MG-63 cells. J Cell Physiol. 153:256–265. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Liu N, Shi S, Deng M, Tang L, Zhang G, Liu N, Ding B, Liu W, Liu Y, Shi H, et al: High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway. J Bone Miner Res. 26:2082–2095. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Derveaux S, Vandesompele J and Hellemans J: How to do successful gene expression analysis using real-time PCR. Methods. 50:227–230. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Schiller ZA, Schiele NR, Sims JK, Lee K and Kuo CK: Adipogenesis of adipose-derived stem cells may be regulated via the cytoskeleton at physiological oxygen levels in vitro. Stem Cell Res Ther. 4:792013. View Article : Google Scholar : PubMed/NCBI

28 

Redshaw Z and Loughna PT: Oxygen concentration modulates the differentiation of muscle stem cells toward myogenic and adipogenic fates. Differentiation. 84:193–202. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Khan WS, Adesida AB, Tew SR, Lowe ET and Hardingham TE: Bone marrow-derived mesenchymal stem cells express the pericyte marker 3G5 in culture and show enhanced chondrogenesis in hypoxic conditions. J Orthop Res. 28:834–840. 2010.PubMed/NCBI

30 

Yang DC, Yang MH, Tsai CC, Huang TF, Chen YH and Hung SC: Hypoxia inhibits osteogenesis in human mesenchymal stem cells through direct regulation of RUNX2 by TWIST. PLoS One. 6:e239652011. View Article : Google Scholar : PubMed/NCBI

31 

Martin-Rendon E, Hale SJ, Ryan D, Baban D, Forde SP, Roubelakis M, Sweeney D, Moukayed M, Harris AL, Davies K and Watt SM: Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia. Stem Cells. 25:1003–1012. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Kim JH, Kim SH, Song SY, Kim WS, Song SU, Yi T, Jeon MS, Chung HM, Xia Y and Sung JH: Hypoxia induces adipocyte differentiation of adipose-derived stem cells by triggering reactive oxygen species generation. Cell Biol Int. 38:32–40. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Zhang QB, Zhang ZQ, Fang SL, Liu YR, Jiang G and Li KF: Effects of hypoxia on proliferation and osteogenic differentiation of periodontal ligament stem cells: An in vitro and in vivo study. Genet Mol Res. 13:10204–10214. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Huang B, Liu LT, Li CQ, Zhuang Y, Luo G, Hu SY and Zhou Y: Study to determine the presence of progenitor cells in the degenerated human cartilage endplates. Eur Spine J. 21:613–622. 2012. View Article : Google Scholar : PubMed/NCBI

35 

D'Ippolito G, Diabira S, Howard GA, Roos BA and Schiller PC: Low oxygen tension inhibits osteogenic differentiation and enhances stemness of human MIAMI cells. Bone. 39:513–522. 2006. View Article : Google Scholar : PubMed/NCBI

36 

Choi JR, Pingguan-Murphy B, Wan Abas WA, Azmi MA Noor, Omar SZ, Chua KH and Wan Safwani WK: Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells. Biochem Biophys Res Commun. 448:218–224. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Brock M, Haider TJ, Vogel J, Gassmann M, Speich R, Trenkmann M, Ulrich S, Kohler M and Huber LC: The hypoxia-induced microRNA-130a controls pulmonary smooth muscle cell proliferation by directly targeting CDKN1A. Int J Biochem Cell Biol. 61:129–137. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Leszczynska KB, Foskolou IP, Abraham AG, Anbalagan S, Tellier C, Haider S, Span PN, O'Neill EE, Buffa FM and Hammond EM: Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. J Clin Invest. 125:2385–2398. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Nerlich AG, Schaaf R, Wälchli B and Boos N: Temporo-spatial distribution of blood vessels in human lumbar intervertebral discs. Eur Spine J. 16:547–555. 2007. View Article : Google Scholar : PubMed/NCBI

40 

Lee DC, Adams CS, Albert TJ, Shapiro IM, Evans SM and Koch CJ: In situ oxygen utilization in the rat intervertebral disc. J Anat. 210:294–303. 2007. View Article : Google Scholar : PubMed/NCBI

41 

Freemont AJ, Watkins A, Le Maitre C, Baird P, Jeziorska M, Knight MT, Ross ER, O'Brien JP and Hoyland JA: Nerve growth factor expression and innervation of the painful intervertebral disc. J Pathol. 197:286–292. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Walsh DA, McWilliams DF, Turley MJ, Dixon MR, Fransès RE, Mapp PI and Wilson D: Angiogenesis and nerve growth factor at the osteochondral junction in rheumatoid arthritis and osteoarthritis. Rheumatology (Oxford). 49:1852–1861. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Babadagli ME, Tezcan B, Yilmaz ST and Tufan AC: Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-β induced chondrogenic differentiation of mesenchymal stem cells. Mol Biol Rep. 41:5549–5555. 2014. View Article : Google Scholar : PubMed/NCBI

44 

Fernandes AM, Herlofsen SR, Karlsen TA, Küchler AM, Fløisand Y and Brinchmann JE: Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage. PLoS One. 8:e629942013. View Article : Google Scholar : PubMed/NCBI

45 

Herlofsen SR, Bryne JC, Høiby T, Wang L, Issner R, Zhang X, Coyne MJ, Boyle P, Gu H, Meza-Zepeda LA, et al: Genome-wide map of quantified epigenetic changes during in vitro chondrogenic differentiation of primary human mesenchymal stem cells. BMC Genomics. 14:1052013. View Article : Google Scholar : PubMed/NCBI

46 

Weber M, Sotoca AM, Kupfer P, Guthke R and van Zoelen EJ: Dynamic modelling of microRNA regulation during mesenchymal stem cell differentiation. BMC Syst Biol. 7:1242013. View Article : Google Scholar : PubMed/NCBI

47 

Blencowe BJ: Alternative splicing: New insights from global analyses. Cell. 126:37–47. 2006. View Article : Google Scholar : PubMed/NCBI

48 

Jeon YM, Kook SH, Rho SJ, Lim SS, Choi KC, Kim HS, Kim JG and Lee JC: Fibroblast growth factor-7 facilitates osteogenic differentiation of embryonic stem cells through the activation of ERK/Runx2 signaling. Mol Cell Biochem. 382:37–45. 2013. View Article : Google Scholar : PubMed/NCBI

49 

Drabek K, van de Peppel J, Eijken M and van Leeuwen JP: GPM6B regulates osteoblast function and induction of mineralization by controlling cytoskeleton and matrix vesicle release. J Bone Miner Res. 26:2045–2051. 2011. View Article : Google Scholar : PubMed/NCBI

50 

Hamidouche Z, Fromigué O, Ringe J, Häupl T and Marie PJ: Crosstalks between integrin alpha 5 and IGF2/IGFBP2 signalling trigger human bone marrow-derived mesenchymal stromal osteogenic differentiation. BMC Cell Biol. 11:442010. View Article : Google Scholar : PubMed/NCBI

51 

Schraufstatter IU, Khaldoyanidi SK and DiScipio RG: Complement activation in the context of stem cells and tissue repair. World J Stem Cells. 7:1090–1108. 2015. View Article : Google Scholar : PubMed/NCBI

52 

Lee DS, Yi TG, Lee HJ, Kim SN, Park S, Jeon MS and Song SU: Mesenchymal stem cells infected with Mycoplasma arginini secrete complement C3 to regulate immunoglobulin production in B lymphocytes. Cell Death Dis. 5:e11922014. View Article : Google Scholar : PubMed/NCBI

53 

Soland MA, Bego M, Colletti E, Zanjani ED, St Jeor S, Porada CD and Almeida-Porada G: Mesenchymal stem cells engineered to inhibit complement-mediated damage. PLoS One. 8:e604612013. View Article : Google Scholar : PubMed/NCBI

54 

Ignatius A, Ehrnthaller C, Brenner RE, Kreja L, Schoengraf P, Lisson P, Blakytny R, Recknagel S, Claes L, Gebhard F, et al: The anaphylatoxin receptor C5aR is present during fracture healing in rats and mediates osteoblast migration in vitro. J Trauma. 71:952–960. 2011. View Article : Google Scholar : PubMed/NCBI

55 

Collard CD, Väkevä A, Morrissey MA, Agah A, Rollins SA, Reenstra WR, Buras JA, Meri S and Stahl GL: Complement activation after oxidative stress: Role of the lectin complement pathway. Am J Pathol. 156:1549–1556. 2000. View Article : Google Scholar : PubMed/NCBI

56 

Cowell RM, Plane JM and Silverstein FS: Complement activation contributes to hypoxic-ischemic brain injury in neonatal rats. J Neurosci. 23:9459–9468. 2003.PubMed/NCBI

57 

Altare F, Jouanguy E, Lamhamedi-Cherradi S, Fondanéche MC, Fizame C, Ribiérre F, Merlin G, Dembic Z, Schreiber R, Lisowska-Grospierre B, et al: A causative relationship between mutant IFNgR1 alleles and impaired cellular response to IFNgamma in a compound heterozygous child. Am J Hum Genet. 62:723–726. 1998. View Article : Google Scholar : PubMed/NCBI

58 

Duque G, Huang DC, Macoritto M, Rivas D, Yang XF, Ste-Marie LG and Kremer R: Autocrine regulation of interferon gamma in mesenchymal stem cells plays a role in early osteoblastogenesis. Stem Cells. 27:550–558. 2009. View Article : Google Scholar : PubMed/NCBI

59 

Duque G, Huang DC, Dion N, Macoritto M, Rivas D, Li W, Yang XF, Li J, Lian J, Marino FT, et al: Interferon-γ plays a role in bone formation in vivo and rescues osteoporosis in ovariectomized mice. J Bone Miner Res. 26:1472–1483. 2011. View Article : Google Scholar : PubMed/NCBI

60 

Dighe AS, Yang S, Madhu V, Balian G and Cui Q: Interferon gamma and T cells inhibit osteogenesis induced by allogeneic mesenchymal stromal cells. J Orthop Res. 31:227–234. 2013. View Article : Google Scholar : PubMed/NCBI

61 

Olszowski T, Poziomkowska-Gęsicka I, Jensenius JC and Adler G: Lectin pathway of complement activation in a Polish woman with MASP-2 deficiency. Immunobiology. 219:261–262. 2014. View Article : Google Scholar : PubMed/NCBI

62 

Choung HW, Lee DS, Lee HK, Shon WJ and Park JC: Preameloblast-derived factors mediate osteoblast differentiation of human bone marrow mesenchymal stem cells by Runx2-Osterix-BSP signaling. Tissue Eng Part A. 22:93–102. 2016. View Article : Google Scholar : PubMed/NCBI

63 

Haddad JJ: Endotoxin-mediated regulation of nuclear factor-kappaB nuclear translocation and activation in the hippocampus of the central nervous system: Modulation by intracerebroventricular treatment with thymulin and the immunomodulatory role of the IkappaB-alpha/pIkappaB-alpha pathway. Neuroscience. 164:1509–1520. 2009. View Article : Google Scholar : PubMed/NCBI

64 

Nguyen TL Xuan, Choi JW, Lee SB, Ye K, Woo SD, Lee KH and Ahn JY: Akt phosphorylation is essential for nuclear translocation and retention in NGF-stimulated PC12 cells. Biochem Biophys Res Commun. 349:789–798. 2006. View Article : Google Scholar : PubMed/NCBI

65 

Mathieu J, Zhang Z, Nelson A, Lamba DA, Reh TA, Ware C and Ruohola-Baker H: Hypoxia induces re-entry of committed cells into pluripotency. Stem Cells. 31:1737–1748. 2013. View Article : Google Scholar : PubMed/NCBI

66 

Cramer T, Schipani E, Johnson RS, Swoboda B and Pfander D: Expression of VEGF isoforms by epiphyseal chondrocytes during low-oxygen tension is HIF-1 alpha dependent. Osteoarthritis Cartilage. 12:433–439. 2004. View Article : Google Scholar : PubMed/NCBI

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Yao Y, Deng Q, Sun C, Song W, Liu H and Zhou Y: A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells. Mol Med Rep 16: 1991-2001, 2017.
APA
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., & Zhou, Y. (2017). A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells. Molecular Medicine Reports, 16, 1991-2001. https://doi.org/10.3892/mmr.2017.6846
MLA
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., Zhou, Y."A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 16.2 (2017): 1991-2001.
Chicago
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., Zhou, Y."A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 16, no. 2 (2017): 1991-2001. https://doi.org/10.3892/mmr.2017.6846
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Deng Q, Sun C, Song W, Liu H and Zhou Y: A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells. Mol Med Rep 16: 1991-2001, 2017.
APA
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., & Zhou, Y. (2017). A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells. Molecular Medicine Reports, 16, 1991-2001. https://doi.org/10.3892/mmr.2017.6846
MLA
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., Zhou, Y."A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 16.2 (2017): 1991-2001.
Chicago
Yao, Y., Deng, Q., Sun, C., Song, W., Liu, H., Zhou, Y."A genome-wide analysis of the gene expression profiles and alternative splicing events during the hypoxia-regulated osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 16, no. 2 (2017): 1991-2001. https://doi.org/10.3892/mmr.2017.6846
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