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AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma

  • Authors:
    • Xiaoyong Wang
    • Chengjun Tao
    • Chengda Yuan
    • Jinping Ren
    • Ming Yang
    • Hangyu Ying
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1964-1972
    |
    Published online on: June 23, 2017
       https://doi.org/10.3892/mmr.2017.6847
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Abstract

Aquaporin 3 (AQP3) and phospholipase D2 (PLD2) are abnormally expressed and/or localized in squamous cell carcinoma (SCC). AQP3 transports glycerol to PLD2 for the synthesis of lipid second messenger, which can mediate the effect of the AQP3/PLD2 signaling module in the regulation of keratinocyte proliferation and differentiation. However, the role of the AQP3/PLD2 signaling module in the pathogenesis of SCC remains to be fully elucidated. In the present study, the expression levels of AQP3 and PLD2 in tissue samples were examined using immunohistochemistry, it was found that the expression levels of AQP3 and PLD2 in tissue samples of actinic keratosis (AK), Bowen's disease (BD) and SCC were significantly increased. AQP3 small interfering RNA (siRNA) and PLD2 siRNA were constructed and used for transfection into the human A431 SCC cell line, and their anticancer effect on SCC was examined. The mRNA expression and protein expression levels of AQP3 and PLD2 were significantly downregulated following siRNA transfection. AQP3 siRNA and PLD2 siRNA inhibited the proliferation and promoted the apoptosis of A431 cells. Taken together, the findings of the present study suggested that increased levels of AQP3 and PLD2 were correlated with tumor progression and development in SCC. AQP3 siRNA and PLD2 siRNA significantly downregulated the mRNA and protein levels of AQP3 and PLD2 in the A431 cells; inhibiting proliferation and promoting apoptosis in vitro. The concomitant effects of AQP3/PLD2 signaling by inhibiting the expression of siRNA may be important for the treatment of SCC in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Tao C, Yuan C, Ren J, Yang M and Ying H: AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma. Mol Med Rep 16: 1964-1972, 2017.
APA
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., & Ying, H. (2017). AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma. Molecular Medicine Reports, 16, 1964-1972. https://doi.org/10.3892/mmr.2017.6847
MLA
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., Ying, H."AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma". Molecular Medicine Reports 16.2 (2017): 1964-1972.
Chicago
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., Ying, H."AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma". Molecular Medicine Reports 16, no. 2 (2017): 1964-1972. https://doi.org/10.3892/mmr.2017.6847
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Tao C, Yuan C, Ren J, Yang M and Ying H: AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma. Mol Med Rep 16: 1964-1972, 2017.
APA
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., & Ying, H. (2017). AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma. Molecular Medicine Reports, 16, 1964-1972. https://doi.org/10.3892/mmr.2017.6847
MLA
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., Ying, H."AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma". Molecular Medicine Reports 16.2 (2017): 1964-1972.
Chicago
Wang, X., Tao, C., Yuan, C., Ren, J., Yang, M., Ying, H."AQP3 small interfering RNA and PLD2 small interfering RNA inhibit the proliferation and promote the apoptosis of squamous cell carcinoma". Molecular Medicine Reports 16, no. 2 (2017): 1964-1972. https://doi.org/10.3892/mmr.2017.6847
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