Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes

  • Authors:
    • Jong Seok Lee
    • Jun Myoung Park
    • Sarah Lee
    • Hye Jin Lee
    • Hee‑Sun Yang
    • Joohong Yeo
    • Ki Rim Lee
    • Byung Hyun Choi
    • Eock Kee Hong
  • View Affiliations

  • Published online on: July 20, 2017     https://doi.org/10.3892/mmr.2017.7042
  • Pages: 4229-4234
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Abstract

Skeletal muscle serves an important role in the utilization of glucose during insulin‑stimulated conditions. Excessive saturated fatty acids are considered to be a major contributing factor to insulin resistance in skeletal muscle cells. The present study investigated the effects of hispidin on palmitate‑induced insulin resistance in C2C12 skeletal muscle myotubes via an MTT assay, glucose uptake assay, Oil‑Red‑O staining and western blot analysis. Hispidin reversed the palmitate‑induced inhibition of glucose uptake, and inhibited palmitate‑induced intracellular lipid accumulation. Hispidin suppressed insulin receptor substrate‑1 Ser307 phosphorylation, and significantly promoted the activation of phosphatidylinositol‑3‑kinase and Akt, via inhibition of protein kinase C theta. Furthermore, hispidin treatment of C2C12 muscle cells increased glucose uptake via activation of adenosine monophosphate‑activated protein kinase. These findings indicated that hispidin may improve palmitate‑induced insulin resistance in skeletal muscle myotubes, and therefore hispidin treatment may be beneficial for patients with diabetes.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lee JS, Park JM, Lee S, Lee HJ, Yang HS, Yeo J, Lee KR, Choi BH and Hong EK: Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes. Mol Med Rep 16: 4229-4234, 2017
APA
Lee, J.S., Park, J.M., Lee, S., Lee, H.J., Yang, H., Yeo, J. ... Hong, E.K. (2017). Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes. Molecular Medicine Reports, 16, 4229-4234. https://doi.org/10.3892/mmr.2017.7042
MLA
Lee, J. S., Park, J. M., Lee, S., Lee, H. J., Yang, H., Yeo, J., Lee, K. R., Choi, B. H., Hong, E. K."Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes". Molecular Medicine Reports 16.4 (2017): 4229-4234.
Chicago
Lee, J. S., Park, J. M., Lee, S., Lee, H. J., Yang, H., Yeo, J., Lee, K. R., Choi, B. H., Hong, E. K."Hispidin rescues palmitate‑induced insulin resistance in C2C12 myotubes". Molecular Medicine Reports 16, no. 4 (2017): 4229-4234. https://doi.org/10.3892/mmr.2017.7042