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Article Open Access

Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses

  • Authors:
    • Heng Liao
    • Lijie Bi
    • Jun Wei
    • Xin Song
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China, Department of Radiology, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8103-8109
    |
    Published online on: September 22, 2017
       https://doi.org/10.3892/mmr.2017.7589
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Abstract

Cytostatic antineoplastic drugs are considered carcinogenic and mutagenic risk factors for health workers who are occupationally exposed to them; however, the molecular mechanisms underlying these effects remain to be elucidated. Therefore, the present study aimed to investigate the underlying mechanisms of antineoplastic drugs‑induced apoptosis of peripheral blood lymphocytes (PBLs) obtained from oncology nurses handling antineoplastic drugs. A microRNA (miRNA/miR) polymerase chain reaction (PCR) array was performed to analyze the expression levels of miRNAs in the PBLs from 3 trained nurses occupationally exposed to antineoplastic drugs. The effects of miR‑34a on cell proliferation and apoptosis in temozolomide (TMZ) treated PBLs were analyzed by cell counting kit‑8 and flow cytometry assays. The protein expression levels of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein, caspase‑3 and caspase‑9 were determined by western blot analysis, and miR‑34a expression levels were detected using quantitative reverse transcription‑PCR. The results of the present study demonstrated that miR‑34a was significantly upregulated in oncology nurses that were occupationally exposed to antineoplastic drugs. In addition, TMZ suppressed cell proliferation and induced apoptosis, by promoting the expression of miR‑34a, in a dose‑dependent manner, and also inhibited the expression of Bcl‑2. Furthermore, knockdown of miR‑34a was able to reverse the reduction of cell proliferation and promotion of apoptosis induced by TMZ in PBLs. Together, these results indicated that abnormal expression of miR‑34a may be considered a diagnostic marker in nurses occupationally exposed to antineoplastic drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Liao H, Bi L, Wei J and Song X: Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses. Mol Med Rep 16: 8103-8109, 2017.
APA
Liao, H., Bi, L., Wei, J., & Song, X. (2017). Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses. Molecular Medicine Reports, 16, 8103-8109. https://doi.org/10.3892/mmr.2017.7589
MLA
Liao, H., Bi, L., Wei, J., Song, X."Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses". Molecular Medicine Reports 16.6 (2017): 8103-8109.
Chicago
Liao, H., Bi, L., Wei, J., Song, X."Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses". Molecular Medicine Reports 16, no. 6 (2017): 8103-8109. https://doi.org/10.3892/mmr.2017.7589
Copy and paste a formatted citation
x
Spandidos Publications style
Liao H, Bi L, Wei J and Song X: Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses. Mol Med Rep 16: 8103-8109, 2017.
APA
Liao, H., Bi, L., Wei, J., & Song, X. (2017). Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses. Molecular Medicine Reports, 16, 8103-8109. https://doi.org/10.3892/mmr.2017.7589
MLA
Liao, H., Bi, L., Wei, J., Song, X."Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses". Molecular Medicine Reports 16.6 (2017): 8103-8109.
Chicago
Liao, H., Bi, L., Wei, J., Song, X."Evaluation of apoptosis induced by exposure to antineoplastic drugs in peripheral blood lymphocytes of nurses". Molecular Medicine Reports 16, no. 6 (2017): 8103-8109. https://doi.org/10.3892/mmr.2017.7589
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