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Article

Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal

  • Authors:
    • Ya‑Fang Wang
    • Zheng‑Hai Tang
    • Ting Li
    • Xiao‑Huang Xu
    • Xiuping Chen
    • Ying Wang
    • Yi‑Tao Wang
    • Jin‑Jian Lu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau 999078, P.R. China
  • Pages: 8392-8398
    |
    Published online on: September 22, 2017
       https://doi.org/10.3892/mmr.2017.7592
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Abstract

Baicalein (BA), one of the major bioactive flavonoids isolated from Scutellariae Radix, possesses various pharmacological activities. The present study aimed to investigate the protective effects of BA on tert‑butyl hydroperoxide (t‑BHP)‑induced hepatotoxicity, and to investigate the potential mechanisms in LO2 cells. BA was demonstrated to possess protective properties against t‑BHP injury in LO2 cells, as evidenced by MTT and lactate dehydrogenase assays. BA significantly prevented t‑BHP‑induced depolarization of mitochondrial membrane potential (MMP), decreased the percentage of apoptotic cells caused by t‑BHP, and prevented intracellular reactive oxygen species (ROS) generation in LO2 cells. Furthermore, BA slightly triggered autophagy in LO2 cells, as evidenced by the elevation of LC3‑II expression, while BA combined treatment with an autophagy inhibitor (chloroquine) or activator (rapamycin) did not alter the hepatoprotective properties. In conclusion, BA may possess a hepatoprotective effect against t‑BHP‑induced liver cell injury, dependent on ROS removal. Therefore, BA may represent a potential drug candidate in protecting hepatotoxicity.
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Copy and paste a formatted citation
Spandidos Publications style
Wang YF, Tang ZH, Li T, Xu XH, Chen X, Wang Y, Wang YT and Lu JJ: Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal. Mol Med Rep 16: 8392-8398, 2017.
APA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y. ... Lu, J. (2017). Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal. Molecular Medicine Reports, 16, 8392-8398. https://doi.org/10.3892/mmr.2017.7592
MLA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16.6 (2017): 8392-8398.
Chicago
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16, no. 6 (2017): 8392-8398. https://doi.org/10.3892/mmr.2017.7592
Copy and paste a formatted citation
x
Spandidos Publications style
Wang YF, Tang ZH, Li T, Xu XH, Chen X, Wang Y, Wang YT and Lu JJ: Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal. Mol Med Rep 16: 8392-8398, 2017.
APA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y. ... Lu, J. (2017). Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal. Molecular Medicine Reports, 16, 8392-8398. https://doi.org/10.3892/mmr.2017.7592
MLA
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16.6 (2017): 8392-8398.
Chicago
Wang, Y., Tang, Z., Li, T., Xu, X., Chen, X., Wang, Y., Wang, Y., Lu, J."Baicalein protects tert‑butyl hydroperoxide‑induced hepatotoxicity dependent of reactive oxygen species removal". Molecular Medicine Reports 16, no. 6 (2017): 8392-8398. https://doi.org/10.3892/mmr.2017.7592
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