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Article

Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production

  • Authors:
    • Lei Zhang
    • Xia Wang
    • Ruixue Wang
    • Xuelian Zheng
    • Na Li
    • Huannan Li
    • Xiaoren Cao
    • Bin Zhou
    • Yong Lin
    • Lan Yang
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Histology and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China, Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Molecular and Translational Medicine, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 8549-8555
    |
    Published online on: September 26, 2017
       https://doi.org/10.3892/mmr.2017.7633
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Abstract

The combination of tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) with other agents has been recognized as a promising strategy to overcome TRAIL resistance in cancer cells. Baicalin (5, 6‑dihydroxy‑7‑o‑glucuronide flavone) is a flavonoid from the root of the medicinal herb Scutellaria baicalensis Georgi, which has been reported to exert antioxidant, anti‑inflammatory, antiviral and anticancer activities in vitro. However, the effect of baicalin on TRAIL‑induced cytotoxicity has not been previously reported. In the present study, the effect of combining TRAIL and baicalin was investigated in non‑small cell lung cancer cell lines. The results revealed that baicalin was able to sensitize A549 and H2009 cells to TRAIL‑induced apoptosis. This was detected by the potentiation of poly‑adenosine‑5'‑diphosphate‑ribose polymerase cleavage and Annexin V‑fluorescein isothiocyanate staining of cells co‑treated with baicalin and TRAIL. In addition, p38 mitogen‑activated protein kinase was activated in baicalin and TRAIL co‑treated cancer cells, whereas the p38 inhibitor SB203580 effectively suppressed cell death within the co‑treated cells. Butylated hydroxyanisole and N‑acetyl‑cysteine, known reactive oxygen species (ROS) scavengers, significantly suppressed the potentiated cytotoxicity induced by baicalin and TRAIL co‑treatment. The present study is the first, to the best of our knowledge, to demonstrate that baicalin enhances the anticancer activity of TRAIL via p38 activation and ROS accumulation, and may be exploited for anticancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Wang X, Wang R, Zheng X, Li N, Li H, Cao X, Zhou B, Lin Y, Yang L, Yang L, et al: Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production. Mol Med Rep 16: 8549-8555, 2017.
APA
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H. ... Yang, L. (2017). Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production. Molecular Medicine Reports, 16, 8549-8555. https://doi.org/10.3892/mmr.2017.7633
MLA
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H., Cao, X., Zhou, B., Lin, Y., Yang, L."Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production". Molecular Medicine Reports 16.6 (2017): 8549-8555.
Chicago
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H., Cao, X., Zhou, B., Lin, Y., Yang, L."Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production". Molecular Medicine Reports 16, no. 6 (2017): 8549-8555. https://doi.org/10.3892/mmr.2017.7633
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Wang X, Wang R, Zheng X, Li N, Li H, Cao X, Zhou B, Lin Y, Yang L, Yang L, et al: Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production. Mol Med Rep 16: 8549-8555, 2017.
APA
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H. ... Yang, L. (2017). Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production. Molecular Medicine Reports, 16, 8549-8555. https://doi.org/10.3892/mmr.2017.7633
MLA
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H., Cao, X., Zhou, B., Lin, Y., Yang, L."Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production". Molecular Medicine Reports 16.6 (2017): 8549-8555.
Chicago
Zhang, L., Wang, X., Wang, R., Zheng, X., Li, N., Li, H., Cao, X., Zhou, B., Lin, Y., Yang, L."Baicalin potentiates TRAIL‑induced apoptosis through p38 MAPK activation and intracellular reactive oxygen species production". Molecular Medicine Reports 16, no. 6 (2017): 8549-8555. https://doi.org/10.3892/mmr.2017.7633
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