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Article Open Access

CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a

  • Authors:
    • Ai‑Gang Sun
    • Fan‑Guo Meng
    • Ming‑Guang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The People's Hospital of Linyi, Linyi, Shandong 276003, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7939-7948
    |
    Published online on: September 27, 2017
       https://doi.org/10.3892/mmr.2017.7642
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Abstract

CDGSH iron sulfur domain 2 (CISD2) has been found to be important in carcinogenesis. However, the role of CISD2 in glioma remains to be elucidated. The present study aimed to investigate the role of CISD2 in glioma using the reverse transcription‑quantitative polymerase chain reaction, western blotting, co‑immunoprecipitation assay, immunofluorescence staining and other methods. The results demonstrated that the mRNA and protein levels of CISD2 were found to be upregulated in glioma tissues, compared with the levels in matched normal tissues. Clinical data analysis showed that the level of CISD2 was negatively correlated with the survival rates of patients with glioma. In addition, high levels of CISD2 were associated with advanced clinical stage, relapse, vascular invasion and increased tumor size. The inhibition of CISD2 suppressed the proliferation and survival of glioma cells in vitro and in vivo. Mechanistically, it was found that small interfering RNA‑induced knock down of CISD2 inhibited the proliferation of glioma cells through activating beclin‑1‑mediated autophagy. The results also revealed that CISD2 was a target of microRNA (miR)‑449a. Together, the results of the present study demonstrated that CISD2 was increased in glioma samples and was associated with poor prognosis and aggressive tumor behavior. The miR‑449a/CISD2/beclin‑1‑mediated autophagy regulatory network contributed to the proliferation of glioma cells. Targeting this pathway may be a promising strategy for glioma therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Sun AG, Meng FG and Wang MG: CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a. Mol Med Rep 16: 7939-7948, 2017.
APA
Sun, A., Meng, F., & Wang, M. (2017). CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a. Molecular Medicine Reports, 16, 7939-7948. https://doi.org/10.3892/mmr.2017.7642
MLA
Sun, A., Meng, F., Wang, M."CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a". Molecular Medicine Reports 16.6 (2017): 7939-7948.
Chicago
Sun, A., Meng, F., Wang, M."CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a". Molecular Medicine Reports 16, no. 6 (2017): 7939-7948. https://doi.org/10.3892/mmr.2017.7642
Copy and paste a formatted citation
x
Spandidos Publications style
Sun AG, Meng FG and Wang MG: CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a. Mol Med Rep 16: 7939-7948, 2017.
APA
Sun, A., Meng, F., & Wang, M. (2017). CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a. Molecular Medicine Reports, 16, 7939-7948. https://doi.org/10.3892/mmr.2017.7642
MLA
Sun, A., Meng, F., Wang, M."CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a". Molecular Medicine Reports 16.6 (2017): 7939-7948.
Chicago
Sun, A., Meng, F., Wang, M."CISD2 promotes the proliferation of glioma cells via suppressing beclin‑1‑mediated autophagy and is targeted by microRNA‑449a". Molecular Medicine Reports 16, no. 6 (2017): 7939-7948. https://doi.org/10.3892/mmr.2017.7642
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