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Article

RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway

  • Authors:
    • Guanhua Chen
    • Xuedong Sun
    • Chunxiao Dong
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Central Hospital of Shengli Oil Field of Shandong, Dongying, Shandong 257000, P.R. China, Department of Pediatrics, Central Hospital of Shengli Oil Field of Shandong, Dongying, Shandong 257000, P.R. China
  • Pages: 8501-8506
    |
    Published online on: September 29, 2017
       https://doi.org/10.3892/mmr.2017.7662
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Abstract

Ras homolog family member A (RhoA) has been reported to be involved in numerous biological processes; however, the effects of RhoA on acute lung injury (ALI) have yet to be reported. The present study aimed to explore how RhoA affects cell viability, reactive oxygen species (ROS) activity and cell apoptosis in a cell model of lipopolysaccharide (LPS)‑induced ALI. An MTT assay, flow cytometry, reverse transcription‑quantitative polymerase chain reaction and western blotting were used to determine the effects of RhoA on cell viability, apoptosis and ROS activity. The results demonstrated that RhoA inactivation was able to promote cell viability, and decrease apoptosis and ROS activity of LPS‑treated cells. The results of western blotting indicated that RhoA activated the downstream Wnt/β‑catenin signaling pathway and inhibited the expression of apoptotic factors. These findings suggested that RhoA may be involved in ALI progression and could be a novel therapeutic target for this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Chen G, Sun X and Dong C: RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway. Mol Med Rep 16: 8501-8506, 2017.
APA
Chen, G., Sun, X., & Dong, C. (2017). RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway. Molecular Medicine Reports, 16, 8501-8506. https://doi.org/10.3892/mmr.2017.7662
MLA
Chen, G., Sun, X., Dong, C."RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway". Molecular Medicine Reports 16.6 (2017): 8501-8506.
Chicago
Chen, G., Sun, X., Dong, C."RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway". Molecular Medicine Reports 16, no. 6 (2017): 8501-8506. https://doi.org/10.3892/mmr.2017.7662
Copy and paste a formatted citation
x
Spandidos Publications style
Chen G, Sun X and Dong C: RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway. Mol Med Rep 16: 8501-8506, 2017.
APA
Chen, G., Sun, X., & Dong, C. (2017). RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway. Molecular Medicine Reports, 16, 8501-8506. https://doi.org/10.3892/mmr.2017.7662
MLA
Chen, G., Sun, X., Dong, C."RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway". Molecular Medicine Reports 16.6 (2017): 8501-8506.
Chicago
Chen, G., Sun, X., Dong, C."RhoA regulates lipopolysaccharide‑induced lung cell injury via the Wnt/β‑catenin pathway". Molecular Medicine Reports 16, no. 6 (2017): 8501-8506. https://doi.org/10.3892/mmr.2017.7662
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