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Article

Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV

  • Authors:
    • Guizhi Jia
    • Bin Leng
    • Hongxin Wang
    • Hongliang Dai
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Pharmacology, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Community Health Nursing, School of Nursing, Jinzhou Medical University, Jinzhou, Liaoning 121001, P.R. China
  • Pages: 8365-8370
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    Published online on: September 29, 2017
       https://doi.org/10.3892/mmr.2017.7676
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Abstract

Astragaloside IV (AsIV), one of the major active ingredients in Astragalus membranaceus, has demonstrated remarkable antifibrotic effects via its antioxidative activity. Cardiac fibrosis is an important pathological mechanism during cardiac remodelling associated with heart failure. In the present study, the mechanism underlying the antifibrotic effect of AsIV upon isoprenaline (ISO) stimulation was investigated. AsIV significantly improved cardiac fibrosis in vivo and dose‑dependently inhibited ISO‑induced CF proliferation in vitro. The ISO‑triggered elevation in reactive oxygen species (ROS) levels was remarkably inhibited by AsIV, as well as ROS scavenger N‑acetylcysteine (NAC), and not affected by cardiotrophin‑1 (CT‑1) knockdown. In addition, AsIV effectively reversed ISO‑induced upregulation of CT‑1 expression, which was blunted by pretreatment with NAC. Cardiac fibroblast (CF) proliferation and collagen Ι overexpression induced by ISO stimulation were effectively abrogated by AsIV, NAC, and CT‑1 small interfering RNA transfection. Taken together, these results demonstrated that AsIV was able to effectively inhibit ISO‑induced CF proliferation and collagen production through negative regulation of ROS‑mediated CT‑1 upregulation.
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Copy and paste a formatted citation
Spandidos Publications style
Jia G, Leng B, Wang H and Dai H: Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV. Mol Med Rep 16: 8365-8370, 2017.
APA
Jia, G., Leng, B., Wang, H., & Dai, H. (2017). Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV. Molecular Medicine Reports, 16, 8365-8370. https://doi.org/10.3892/mmr.2017.7676
MLA
Jia, G., Leng, B., Wang, H., Dai, H."Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV". Molecular Medicine Reports 16.6 (2017): 8365-8370.
Chicago
Jia, G., Leng, B., Wang, H., Dai, H."Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV". Molecular Medicine Reports 16, no. 6 (2017): 8365-8370. https://doi.org/10.3892/mmr.2017.7676
Copy and paste a formatted citation
x
Spandidos Publications style
Jia G, Leng B, Wang H and Dai H: Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV. Mol Med Rep 16: 8365-8370, 2017.
APA
Jia, G., Leng, B., Wang, H., & Dai, H. (2017). Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV. Molecular Medicine Reports, 16, 8365-8370. https://doi.org/10.3892/mmr.2017.7676
MLA
Jia, G., Leng, B., Wang, H., Dai, H."Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV". Molecular Medicine Reports 16.6 (2017): 8365-8370.
Chicago
Jia, G., Leng, B., Wang, H., Dai, H."Inhibition of cardiotrophin‑1 overexpression is involved in the anti‑fibrotic effect of Astrogaloside IV". Molecular Medicine Reports 16, no. 6 (2017): 8365-8370. https://doi.org/10.3892/mmr.2017.7676
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