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Article

miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis

  • Authors:
    • Ying Ma
    • Yu‑Xin Huang
    • Yan‑Ying Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology and Obstetrics, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
  • Pages: 8259-8264
    |
    Published online on: September 29, 2017
       https://doi.org/10.3892/mmr.2017.7677
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Abstract

Endometrial-derived stem cells (EnSCs) serve an important role in the development of endometriosis via retrograde menstruation. Abnormal expression of miRNAs in EnSCs is involved in the etiology of endometriosis, however, the mechanisms remain unclear. The aim of the present study was to investigate the expression of miR‑34a‑5p and VEGFA in endometrial samples from patients with or without endometriosis, and then examine the underlying mechanism of microRNA‑34a‑5p regulation of VEGFA in EnSCs. Endometrial samples from patients with or without endometriosis were collected, and miR‑34a‑5p expression in the two groups was measured using RT‑PCR. Human endometrial‑derived stem cells (hEnSCs) were isolated from these endometrial samples, and hEnSCs were transfected with the miR‑34a‑5p mimics or control miRNAs. qPCR and western blotting were performed to assess the effects of miR‑34a‑5p on the expression of VEGFA in hEnSCs, and cell growth was assessed by an MTT assay. miR‑34a‑5p was significantly downregulated in patients with endometriosis when compared with that of those without endometriosis. VEGFA expression levels in hEnSCs with an overexpression of miR‑34a‑5p were significantly reduced when compared with those in the negative control (P<0.01). In addition, the upregulation of miR‑34a‑5p suppressed EnSCs proliferation by targeting the 3' untranslated region of VEGFA. miR‑34a‑5p provides a novel avenue for the understanding of the development of endometriosis, and may facilitate the development of potential therapeutics against endometriosis.
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1 

Yoder N, Tal R and Martin JR: Abdominal ectopic pregnancy after in vitro fertilization and single embryo transfer: A case report and systematic review. Reprod Biol Endocrinol. 14:692016. View Article : Google Scholar : PubMed/NCBI

2 

Bulun SE: Endometriosis. N Engl J Med. 360:268–279. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Dinulescu DM, Ince TA, Quade BJ, Shafer SA, Crowley D and Jacks T: Role of K-ras and Pten in the development of mouse models of endometriosis and endometrioid ovarian cancer. Nat Med. 11:63–70. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Greene AD, Lang SA, Kendziorski JA, Sroga-Rios JM, Herzog TJ and Burns KA: Endometriosis: Where are we and where are we going? Reproduction. 152:R63–R78. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Maruyama T and Yoshimura Y: Stem cell theory for the pathogenesis of endometriosis. Front Biosci. 4:2754–2763. 2012. View Article : Google Scholar

6 

Sasson IE and Taylor HS: Stem cells and the pathogenesis of endometriosis. Ann N Y Acad Sci. 1127:106–115. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Kao AP, Wang KH, Chang CC, Lee JN, Long CY, Chen HS, Tsai CF, Hsieh TH and Tsai EM: Comparative study of human eutopic and ectopic endometrial mesenchymal stem cells and the development of an in vivo endometriotic invasion model. Fertil Steril. 95:1308–1315. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Groothuis PG, Nap AW, Winterhager E and Grümmer R: Vascular development in endometriosis. Angiogenesis. 8:147–156. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Teague EM, Print CG and Hull ML: The role of microRNAs in endometriosis and associated reproductive conditions. Hum Reprod Update. 16:142–165. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Zou Y, Guo CG and Zhang MM: Inhibition of human hepatocellular carcinoma tumor angiogenesis by siRNA silencing of VEGF via hepatic artery perfusion. Eur Rev Med Pharmacol Sci. 19:4751–4761. 2015.PubMed/NCBI

11 

Liu B, Ding JF, Luo J, Lu L, Yang F and Tan XD: Seven protective miRNA signatures for prognosis of cervical cancer. Oncotarget. 7:56690–56698. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Cho S, Mutlu L, Grechukhina O and Taylor HS: Circulating microRNAs as potential biomarkers for endometriosis. Fertil Steril. 103:1252–1260. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Toloubeydokhti T, Pan Q, Luo X, Bukulmez O and Chegini N: The expression and ovarian steroid regulation of endometrial micro-RNAs. Reprod Sci. 15:993–1001. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Braza-Boïls A, Salloum-Asfar S, Marí-Alexandre J, Arroyo AB, González-Conejero R, Barceló-Molina M, García-Oms J, Vicente V, Estellés A, Gilabert-Estellés and Martínez C: Peritoneal fluid modifies the microRNA expression profile in endometrial and endometriotic cells from women with endometriosis. Hum Reprod. 30:2292–2302. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Gao J, Li N, Dong Y, Li S, Xu L, Li X, Li Y, Li Z, Ng SS, Sung JJ, et al: miR-34a-5p suppresses colorectal cancer metastasis and predicts recurrence in patients with stage II/III colorectal cancer. Oncogene. 34:4142–4152. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Scognamiglio I, Di Martino MT, Campani V, Virgilio A, Galeone A, Gullà A, Cantafio ME Gallo, Misso G, Tagliaferri P, Tassone P, et al: Transferrin-conjugated SNALPs encapsulating 2′-O-methylated miR-34a for the treatment of multiple myeloma. Biomed Res Int. Biomed Res Int. 2014:2173652014. View Article : Google Scholar : PubMed/NCBI

17 

Di Martino MT, Campani V, Misso G, Cantafio ME Gallo, Gullà A, Foresta U, Guzzi PH, Castellano M, Grimaldi A, Gigantino V, et al: In vivo activity of miR-34a mimics delivered by stable nucleic acid lipid particles (SNALPs) against multiple myeloma. PLoS One. 9:e900052014. View Article : Google Scholar : PubMed/NCBI

18 

Misso G, Di Martino MT, De Rosa G, Farooqi AA, Lombardi A, Campani V, Zarone MR, Gullà A, Tagliaferri P, Tassone P and Caraglia M: Mir-34: A new weapon against cancer? Mol Ther Nucleic Acids. 3:e1942014. View Article : Google Scholar : PubMed/NCBI

19 

Song P, Ye LF, Zhang C, Peng T and Zhou XH: Long non-coding RNA XIST exerts oncogenic functions in human nasopharyngeal carcinoma by targeting miR-34a-5p. Gene. 592:8–14. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Li XY, Wen JY, Jia CC, Wang TT, Li X, Dong M, Lin QU, Chen ZH, Ma XK, Wei LI, et al: MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97 L cells. Oncol Lett. 10:2691–2698. 2015.PubMed/NCBI

21 

Ma ZB, Kong XL, Cui G, Ren CC, Zhang YJ, Fan SJ and Li YH: Expression and clinical significance of miRNA-34a in colorectal cancer. Asian Pac J Cancer Prev. 15:9265–9270. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Walton SJ, Lewis A, Jeffery R, Thompson H, Feakins R, Giannoulatou E, Yau C, Lindsay JO, Clark SK and Silver A: Familial adenomatous patients with desmoid tumours show increased expression of miR-34a in serum and high levels in tumours. Oncoscience. 3:173–185. 2016.PubMed/NCBI

23 

Lu H, Hao L, Li S, Lin S, Lv L, Chen Y, Cui H, Zi T, Chu X, Na L and Sun C: Elevated circulating stearic acid leads to a major lipotoxic effect on mouse pancreatic beta cells in hyperlipidaemia via a miR-34a-5p-mediated PERK/p53-dependent pathway. Diabetologia. 59:1247–1257. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Goel HL and Mercurio AM: VEGF targets the tumour cell. Nat Rev Cancer. 13:871–882. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Li YZ, Wang LJ, Li X, Li SL, Wang JL, Wu ZH, Gong L and Zhang XD: Vascular endothelial growth factor gene polymorphisms contribute to the risk of endometriosis: An updated systematic review and meta-analysis of 14 case-control studies. Genet Mol Res. 12:1035–1044. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Ramón LA, Braza-Boïls A, Gilabert-Estellés J, Gilabert J, España F, Chirivella M and Estellés A: MicroRNAs expression in endometriosis and their relation to angiogenic factors. Hum Reprod. 26:1082–1090. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Zhang D, Zhou J and Dong M: Dysregulation of microRNA-34a expression in colorectal cancer inhibits the phosphorylation of FAK via VEGF. Dig Dis Sci. 59:958–967. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Cheng J, Chen Y, Zhao P, Liu X, Dong J, Li J, Huang C, Wu R and Lv Y: Downregulation of miRNA-638 promotes angiogenesis and growth of hepatocellular carcinoma by targeting VEGF. Oncotarget. 7:30702–30711. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Ueno K, Samura M, Nakamura T, Tanaka Y, Takeuchi Y, Kawamura D, Takahashi M, Hosoyama T, Morikage N and Hamano K: Increased plasma VEGF levels following ischemic preconditioning are associated with downregulation of miRNA-762 and miR-3072-5p. Sci Rep. 6:367582016. View Article : Google Scholar : PubMed/NCBI

30 

Kim SH, Choi YM, Chae HD, Kim CH and Kang BM: Decreased expression of angiogenin in the eutopic endometrium from women with advanced stage endometriosis. J Korean Med Sci. 23:802–807. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Finn NA and Searles CD: Intracellular and extracellular miRNAs in regulation of angiogenesis signaling. Curr Angiogenes. 4:299–307. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Hsu CY, Hsieh TH, Tsai CF, Tsai HP, Chen HS, Chang Y, Chuang HY, Lee JN, Hsu YL and Tsai EM: miRNA-199a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis. J Pathol. 232:330–343. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Zhu X, Er K, Mao C, Yan Q, Xu H, Zhang Y, Zhu J, Cui F, Zhao W and Shi H: miR-203 suppresses tumor growth and angiogenesis by targeting VEGFA in cervical cancer. Cell Physiol Biochem. 32:64–73. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Huang YX and Chen YY: miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis. Mol Med Rep 16: 8259-8264, 2017.
APA
Ma, Y., Huang, Y., & Chen, Y. (2017). miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis. Molecular Medicine Reports, 16, 8259-8264. https://doi.org/10.3892/mmr.2017.7677
MLA
Ma, Y., Huang, Y., Chen, Y."miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis". Molecular Medicine Reports 16.6 (2017): 8259-8264.
Chicago
Ma, Y., Huang, Y., Chen, Y."miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis". Molecular Medicine Reports 16, no. 6 (2017): 8259-8264. https://doi.org/10.3892/mmr.2017.7677
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Huang YX and Chen YY: miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis. Mol Med Rep 16: 8259-8264, 2017.
APA
Ma, Y., Huang, Y., & Chen, Y. (2017). miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis. Molecular Medicine Reports, 16, 8259-8264. https://doi.org/10.3892/mmr.2017.7677
MLA
Ma, Y., Huang, Y., Chen, Y."miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis". Molecular Medicine Reports 16.6 (2017): 8259-8264.
Chicago
Ma, Y., Huang, Y., Chen, Y."miRNA‑34a‑5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis". Molecular Medicine Reports 16, no. 6 (2017): 8259-8264. https://doi.org/10.3892/mmr.2017.7677
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