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Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway

  • Authors:
    • Lin Yang
    • Jianlin Liu
    • Guangyu Qi
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8868-8874
    |
    Published online on: October 3, 2017
       https://doi.org/10.3892/mmr.2017.7691
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Abstract

The present study aimed to investigate the effects of saikosaponin on oxidized low‑density lipoprotein (ox‑LDL)‑induced human umbilical vein endothelial cell (HUVEC) injury and apoptosis, and examine the involvement of the mitogen‑activated protein kinase (MAPK) signaling pathway. The viability and apoptosis of HUVECs were detected using an MTT assay and flow cytometry. ELISA analysis was applied to measure the levels of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 cytokines. Nuclear factor (NF)‑κB p65 nuclear translocation was observed using immunofluorescence staining. The levels of intercellular adhesion molecule 1 and vascular cell adhesion molecule‑1 were detected using reverse transcription‑polymerase chain reaction analysis. The phosphorylation of B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), caspase‑3 p38, c‑Jun N‑terminal kinase (JNK) and extracellular signal‑regulated kinase (ERK)1/2 were detected using western blot analysis. The results revealed that saikosaponin increased the viability of the HUVECs and decreased the early‑stage apoptotic rate of the HUVECs induced by ox‑LDL. The expression levels of inflammatory cytokines in the injured vascular endothelial cells were decreased, the expression levels of adhesion molecules were reduced, the activity of superoxide dismutase was increased, and malondialdehyde content was decreased. Therefore, the inflammatory response and oxidative stress were inhibited. Simultaneously, the levels of Bcl‑2 increased, the levels of Bax and caspase‑3 decreased, and the nuclear translocation of NF‑κB p65 was significantly inhibited. The protein levels of phosphorylated p38 and JNK were reduced, whereas that of ERK1/2 remained unaffected. It was concluded that the MAPK signaling pathway mediated HUVEC injury induced by ox‑LDL. However, saikosaponin inhibited the HUVEC injury induced by ox‑LDL through inhibiting the ERK1/2 and p38 MAPK signaling pathways, and possibly also through the JNK and p38 MAPK signaling pathway.
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1 

Lee SM, Lee YJ, Kim YC, Kim JS, Kang DG and Lee HS: Vascular protective role of vitexicarpin isolated from Vitex rotundifolia in human umbilical vein endothelial cells. Inflammation. 35:584–593. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Madonna R, Massaro M, Pandolfi A, Consoli A and De Caterina R: The prominent role of p38 mitogen-activated protein kinase in insulin-mediated enhancement of VCAM-1 expression in endothelial cells. Int J Immunopathol Pharmacol. 20:539–555. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Wang Z, Niu Q, Peng X, Li M, Liu Y, Liu J, Wen S and Wei Y: Mitofusin 2 ameliorates aortic remodeling by suppressing ras/raf/ERK pathway and regulating mitochondrial function in vascular smooth muscle cells. Int J Cardiol. 178:165–167. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Park B, Yim JH, Lee HK, Kim BO and Pyo S: Ramalin inhibits VCAM-1 expression and adhesion of monocyte to vascular smooth muscle cells through MAPK and PADI4-dependent NF-κB and AP-1 pathways. Biosci Biotechnol Biochem. 79:539–552. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Martin-Blanco E: p38 MAPK signalling cascades: Ancient roles and new functions. Bioessays. 22:637–645. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Chahine MN, Blackwood DP, Dibrov E, Richard MN and Pierce GN: Oxidized LDL affects smooth muscle cell growth through MAPK-mediated actions on nuclear protein import. J Mol Cell Cardiol. 46:431–441. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Wang GF, Shi CG, Sun MZ, Wang L, Wu SX, Wang HF, Xu ZQ and Chen DM: Tetramethylpyrazine attenuates atherosclerosis development and protects endothelial cells from ox-LDL. Cardiovasc Drugs Ther. 27:199–210. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Fisk M, Gajendragadkar PR, Mäki-Petäjä KM, Wilkinson IB and Cheriyan J: Therapeutic potential of p38 MAP kinase inhibition in the management of cardiovascular disease. Am J Cardiovasc Drugs. 14:155–165. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Sun Y, Liu WZ, Liu T, Feng X, Yang N and Zhou HF: Signaling pathway of MAPK/ERK in cell proliferation, differentiation, migration, senescence and apoptosis. J Recept Signal Transduct Res. 35:600–604. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Glaros EN, Kim WS and Garner B: Myriocin-mediated up-regulation of hepatocyte apoA-I synthesis is associated with ERK inhibition. Clin Sci (Lond). 118:727–736. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Dhanasekaran DN and Reddy EP: JNK signaling in apoptosis. Oncogene. 27:6245–6251. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Yarza R, Vela S, Solas M and Ramirez MJ: c-Jun N-terminal Kinase (JNK) signaling as a therapeutic target for Alzheimer's disease. Front Pharmacol. 6:3212016. View Article : Google Scholar : PubMed/NCBI

13 

Wei L, Deng W, Cheng Z, Guo H, Wang S, Zhang X, He Y and Tang Q: Effects of hesperetin on platelet-derived growth factor-BB-induced pulmonary artery smooth muscle cell proliferation. Mol Med Rep. 13:955–960. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Cui Y, Sun YW, Lin HS, Su WM, Fang Y, Zhao Y, Wei XQ, Qin YH, Kohama K and Gao Y: Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways. Mol Cell Biochem. 393:255–263. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Kim MH, Kang HM, Kim CE, Han S and Kim SW: Ramipril inhibits high glucose-stimulated up-regulation of adhesion molecules via the ERK1/2 MAPK signaling pathway in human umbilical vein endothelial cells. Cell Mol Biol Lett. 20:937–947. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Liu J and Qi G: Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway. Mol Med Rep 16: 8868-8874, 2017.
APA
Yang, L., Liu, J., & Qi, G. (2017). Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway. Molecular Medicine Reports, 16, 8868-8874. https://doi.org/10.3892/mmr.2017.7691
MLA
Yang, L., Liu, J., Qi, G."Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway". Molecular Medicine Reports 16.6 (2017): 8868-8874.
Chicago
Yang, L., Liu, J., Qi, G."Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway". Molecular Medicine Reports 16, no. 6 (2017): 8868-8874. https://doi.org/10.3892/mmr.2017.7691
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Liu J and Qi G: Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway. Mol Med Rep 16: 8868-8874, 2017.
APA
Yang, L., Liu, J., & Qi, G. (2017). Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway. Molecular Medicine Reports, 16, 8868-8874. https://doi.org/10.3892/mmr.2017.7691
MLA
Yang, L., Liu, J., Qi, G."Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway". Molecular Medicine Reports 16.6 (2017): 8868-8874.
Chicago
Yang, L., Liu, J., Qi, G."Mechanism of the effect of saikosaponin on atherosclerosis in vitro is based on the MAPK signaling pathway". Molecular Medicine Reports 16, no. 6 (2017): 8868-8874. https://doi.org/10.3892/mmr.2017.7691
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