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Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro

  • Authors:
    • Yong Cheng
    • Zhiwei Qu
    • Ximeng Fu
    • Qi Jiang
    • Jianfeng Fei
  • View Affiliations / Copyright

    Affiliations: Department of Bone Surgery, The First Hospital of Harbin City, Harbin, Heilongjiang 150010, P.R. China, Department of Plastic Surgery, The First Hospital of Harbin City, Harbin, Heilongjiang 150010, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8826-8832
    |
    Published online on: October 3, 2017
       https://doi.org/10.3892/mmr.2017.7701
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Abstract

A variety of pulsed electromagnetic fields (PEMFs) have been experimentally and clinically used in an effort to promote wound healing, although the mechanisms involved remain unknown. The aim of the present study was to investigate the action of a novel protocol of co‑treatment with PEMFs and hydroxytyrosol (HTY) on the proliferation and differentiation potential of human umbilical vein endothelial cells (HUVECs). The HUVECs were assigned randomly into three groups: Control, PEMF‑treated and PEMF + HT‑treated. The intensity of the electromagnetic field used in this protocol was 2.25 mT, the frequency of the bursts was 50 Hz and the application time was 15 min. A Cell Counting kit‑8 (CCK‑8) assay was used to assess cell proliferation, and cell apoptosis was analyzed by TUNEL apoptosis assay kit and calcein‑acetoxymethyl/propidium iodide dual‑staining assay. In addition, protein and mRNA expression levels of protein kinase B (Akt), mechanistic target of rapamycin (mTOR), transforming growth factor (TGF)‑β1 and p53 were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction assays, respectively. The CCK‑8 assay demonstrated that HTY contributed to HUVEC proliferation mediated by PEMFs in a time‑dependent manner. The Transwell assay and scratch wound results demonstrated that co‑treatment of HTY and PEMFs could increase HUVEC migration. Furthermore, the levels of apoptotic cells were reversed by pre‑incubation with HTY in the PEMF treatment group, while PEMF treatment alone had no such effect. The proteins and mRNA expression levels of Akt, mTOR, TGF‑β1 were elevated in co‑treatment of HTY and PEMFs, whereas there was no effect on levels of p53. Therefore, the results indicated that combined exposure of HUVECs to PEMFs and HTY exerted protective effects in HUVECs by promoting cell proliferation and inhibiting apoptosis. In conclusion, to the best of our knowledge, the present study was the first to demonstrate the beneficial roles of HTY and PEMF combined treatment in HUVECs, which may represent an effective treatment for wound healing.
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1 

Janis JE, Attinger CE and Lavery L: Introduction to ‘current concepts in wound healing: Update 2016’. Plast Reconstr Surg. 138(3 Suppl): 7S–8S. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Yoshida S, Yoshimoto H, Hirano A and Akita S: Wound healing and angiogenesis through combined use of a vascularized tissue flap and adipose-derived stem cells in a rat hindlimb irradiated ischemia model. Plast Reconstr Surg. 137:1486–1497. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Athanasiou A, Karkambounas S, Batistatou A, Lykoudis E, Katsaraki A, Kartsiouni T, Papalois A and Evangelou A: The effect of pulsed electromagnetic fields on secondary skin wound healing: Aexperimental study. Bioelectromagnetics. 28:362–368. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Monache S Delle, Alessandro R, Iorio R, Gualtieri G and Colonna R: Extremely low frequency electromagnetic fields (ELF-EMFs) induce in vitro angiogenesis process in human endothelial cells. Bioelectromagnetics. 29:640–648. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Li F, Lei T, Xie K, Wu X, Tang C, Jiang M, Liu J, Luo E and Shen G: Effects of extremely low frequency pulsed magnetic fields on diabetic nephropathy in streptozotocin-treated rats. Biomed Eng Online. 15:82016. View Article : Google Scholar : PubMed/NCBI

6 

Martino CF, Perea H, Hopfner U, Ferguson VL and Wintermantel E: Effects of weak static magnetic fields on endothelial cells. Bioelectromagnetics. 31:296–301. 2010.PubMed/NCBI

7 

Li RL, Huang JJ, Shi YQ, Hu A, Lu ZY, Weng L, Wang SQ, Han YP, Zhang L, Hao CN and Duan JL: Pulsed electromagnetic field improves postnatal neovascularization in response to hindlimb ischemia. Am J Transl Res. 7:430–444. 2015.PubMed/NCBI

8 

Cheing GL, Li X, Huang L, Kwan RL and Cheung KK: Pulsed electromagnetic fields (PEMF) promote early wound healing and myofibroblast proliferation in diabetic rats. Bioelectromagnetics. 35:161–169. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Goudarzi I, Hajizadeh S, Salmani ME and Abrari K: Pulsed electromagnetic fields accelerate wound healing in the skin of diabetic rats. Bioelectromagnetics. 31:318–323. 2010.PubMed/NCBI

10 

Lamy S, Ben Saad A, Zgheib A and Annabi B: Olive oil compounds inhibit the paracrine regulation of TNF-alpha-induced endothelial cell migration through reduced glioblastoma cell cyclooxygenase-2 expression. J Nutr Biochem. 27:136–145. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Morbidelli L: Polyphenol-based nutraceuticals for the control of angiogenesis: Analysis of the critical issues for human use. Pharmacol Res. 111:384–393. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Zrelli H, Kusunoki M and Miyazaki H: Role of hydroxytyrosol-dependent regulation of HO-1 expression in promoting wound healing of vascular endothelial cells via Nrf2 De Novo synthesis and stabilization. Phytother Res. 29:1011–1018. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Scoditti E, Calabriso N, Massaro M, Pellegrino M, Storelli C, Martines G, De Caterina R and Carluccio MA: Mediterranean diet polyphenols reduce inflammatory angiogenesis through MMP-9 and COX-2 inhibition in human vascular endothelial cells: A potentially protective mechanism in atherosclerotic vascular disease and cancer. Arch Biochem Biophys. 527:81–89. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Ferroni L, Bellin G, Emer V, Rizzuto R, Isola M, Gardin C and Zavan B: Treatment by Therapeutic Magnetic Resonance (TMR) increases fibroblastic activity and keratinocyte differentiation in an in vitro model of 3D artificial skin. J Tissue Eng Regenerat med. 11:1332–1342. 2015. View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Goto T, Fujioka M, Ishida M, Kuribayashi M, Ueshima K and Kubo T: Noninvasive up-regulation of angiopoietin-2 and fibroblast growth factor-2 in bone marrow by pulsed electromagnetic field therapy. J Orthop Sci. 15:661–665. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Osti L, Buono AD and Maffulli N: Pulsed electromagnetic fields after rotator cuff repair: A randomized, controlled study. Orthopedics. 38:e223–e228. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Kim YT, Hei WH, Kim S, Seo YK, Kim SM, Jahng JW and Lee JH: Co-treatment effect of pulsed electromagnetic field (PEMF) with human dental pulp stromal cells and FK506 on the regeneration of crush injured rat sciatic nerve. Int J Neurosci. 125:774–783. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Bagnato GL, Miceli G, Marino N, Sciortino D and Bagnato GF: Pulsed electromagnetic fields in knee osteoarthritis: A double blind, placebo-controlled, randomized clinical trial. Rheumatology (Oxford). 55:755–762. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Menini M, Bevilacqua M, Setti P, Tealdo T, Pesce P and Pera P: Effects of pulsed electromagnetic fields on swelling and pain after implant surgery: A double-blind, randomized study. Int J Oral Maxillofac Surg. 45:346–353. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Guerriero F, Botarelli E, Mele G, Polo L, Zoncu D, Renati P, Sgarlata C, Rollone M, Ricevuti G, Maurizi N, et al: Effectiveness of an innovative pulsed electromagnetic fields stimulation in healing of untreatable skin ulcers in the frail elderly: Two case reports. Case Rep Dermatol Med. 2015:5765802015.PubMed/NCBI

22 

Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ and Solà R: Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells. Mol Nutr Food Res. 59:2523–2536. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cheng Y, Qu Z, Fu X, Jiang Q and Fei J: Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro. Mol Med Rep 16: 8826-8832, 2017.
APA
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., & Fei, J. (2017). Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro. Molecular Medicine Reports, 16, 8826-8832. https://doi.org/10.3892/mmr.2017.7701
MLA
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., Fei, J."Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro". Molecular Medicine Reports 16.6 (2017): 8826-8832.
Chicago
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., Fei, J."Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro". Molecular Medicine Reports 16, no. 6 (2017): 8826-8832. https://doi.org/10.3892/mmr.2017.7701
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Y, Qu Z, Fu X, Jiang Q and Fei J: Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro. Mol Med Rep 16: 8826-8832, 2017.
APA
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., & Fei, J. (2017). Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro. Molecular Medicine Reports, 16, 8826-8832. https://doi.org/10.3892/mmr.2017.7701
MLA
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., Fei, J."Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro". Molecular Medicine Reports 16.6 (2017): 8826-8832.
Chicago
Cheng, Y., Qu, Z., Fu, X., Jiang, Q., Fei, J."Hydroxytyrosol contributes to cell proliferation and inhibits apoptosis in pulsed electromagnetic fields treated human umbilical vein endothelial cells in vitro". Molecular Medicine Reports 16, no. 6 (2017): 8826-8832. https://doi.org/10.3892/mmr.2017.7701
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