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Article

Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation

  • Authors:
    • Dahai Yu
    • Yan Zhang
    • Hong Lu
    • Di Zhao
  • View Affiliations / Copyright

    Affiliations: Jiangsu Collaborative Innovation Center of Tumor Prevention and Treatment by Traditional Chinese Medicine (TCM), Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, Department of Clinical Laboratory, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 9005-9010
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7704
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Abstract

Radiotherapy is the major form of treatment for head and neck carcinoma, a malignant tumour of epithelial origin. The identification of agents, which can be co‑administered in order to sensitize these tumours to radiotherapy, has become a major focus of investigations. In the present study, a novel 20 nm nanocomposite, Ag/C225, was constructed, which consisted of silver nanoparticles (AgNPs) conjugated to an epidermal growth factor receptor‑specific antibody (C225). Physical characterization demonstrated that the Ag/C225 nanoparticles were spherical and dispersed well in water. Enzyme‑linked immunosorbent assays showed that the activity of C225 was preserved in the Ag/C225 nanoparticles. The results of 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide analysis revealed that AgNPs and Ag/C225 inhibited the proliferation of nasopharyngeal carcinoma epithelial (CNE) cells in a dose‑ and time‑dependent manner. Flow cytometry revealed that AgNPs and Ag/C225 induced the apoptosis of CNEs, and abrogated G2 arrest; the latter effect was more marked with Ag/C225 than with AgNPs. Clonogenic assays indicated that AgNPs and Ag/C225 increased the sensitivity of CNEs to irradiation. The sensitizer enhancement ratios were 1.610±0.012 and 1.405±0.033 Gy for AgNPs and Ag/C225, respectively. Western blot analysis revealed that combining X‑ray irradiation with either AgNPs or Ag/C225 reduced the expression levels of DNA damage/repair proteins Ku‑70, Ku‑80 and Rad51; Ag/C225 was also more effective than AgNPs in this context. These results indicated that AgNPs and Ag/C225 effectively enhanced CNE cell radiosensitivity in vitro. Therefore, these potent agents may be considered for use as radiosensitizers during the treatment of human nasopharyngeal carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Yu D, Zhang Y, Lu H and Zhao D: Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation. Mol Med Rep 16: 9005-9010, 2017.
APA
Yu, D., Zhang, Y., Lu, H., & Zhao, D. (2017). Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation. Molecular Medicine Reports, 16, 9005-9010. https://doi.org/10.3892/mmr.2017.7704
MLA
Yu, D., Zhang, Y., Lu, H., Zhao, D."Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation". Molecular Medicine Reports 16.6 (2017): 9005-9010.
Chicago
Yu, D., Zhang, Y., Lu, H., Zhao, D."Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation". Molecular Medicine Reports 16, no. 6 (2017): 9005-9010. https://doi.org/10.3892/mmr.2017.7704
Copy and paste a formatted citation
x
Spandidos Publications style
Yu D, Zhang Y, Lu H and Zhao D: Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation. Mol Med Rep 16: 9005-9010, 2017.
APA
Yu, D., Zhang, Y., Lu, H., & Zhao, D. (2017). Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation. Molecular Medicine Reports, 16, 9005-9010. https://doi.org/10.3892/mmr.2017.7704
MLA
Yu, D., Zhang, Y., Lu, H., Zhao, D."Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation". Molecular Medicine Reports 16.6 (2017): 9005-9010.
Chicago
Yu, D., Zhang, Y., Lu, H., Zhao, D."Silver nanoparticles coupled to anti‑EGFR antibodies sensitize nasopharyngeal carcinoma cells to irradiation". Molecular Medicine Reports 16, no. 6 (2017): 9005-9010. https://doi.org/10.3892/mmr.2017.7704
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