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Article

MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma

  • Authors:
    • Lu Jin
    • Yifan Li
    • Liping Nie
    • Tao He
    • Jia Hu
    • Jiaju Liu
    • Mingwei Chen
    • Min Shi
    • Zhimao Jiang
    • Yaoting Gui
    • Shangqi Yang
    • Yongqing Lai
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China, Department of Clinical Medicine, Second Clinical Medical College, Anhui Medical University, Hefei, Anhui 230032, P.R. China, The Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
  • Pages: 9157-9164
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7705
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Abstract

Micro (mi)RNAs are involved in multiple cellular processes, and alterations in miRNA expression have been demonstrated to lead to tumorigenesis. Previous microarray analysis revealed that miRNA (miR)‑24 was downregulated in renal cell carcinoma (RCC). Additionally, miR‑24 has been identified as an oncogene and tumor suppressor in various cancers. The present study assessed the expression levels of two stem‑loops of miR‑24, miR‑24‑1 and miR‑24‑2, in RCC tissues and paired healthy tissues by reverse transcription‑quantitative polymerase chain reaction. The results revealed that miR‑24‑2 was upregulated in RCC tissues and ACHN, 786‑O and 769P cell lines compared with healthy tissues and HEK‑293T cells, respectively, whereas miR‑24‑1 was almost absent in RCC and healthy kidney tissues. To investigate the role of miR‑24‑2 in RCC, a synthesized miR‑24‑2 mimic, negative control (NC), inhibitor or inhibitor NC was transfected into 786‑O and ACHN RCC cells, and cell proliferation, mobility and apoptosis assays were performed. The results of the present study revealed that miR‑24‑2 was associated with cell proliferation, migration, invasion and apoptosis, thus demonstrating that miR‑24‑2 may serve a role as an oncogene in RCC. Further studies are required to investigate the signaling pathways of miR‑24‑2, and the potential of miR‑24‑2 as a therapeutic target or biomarker for the early detection of RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Jin L, Li Y, Nie L, He T, Hu J, Liu J, Chen M, Shi M, Jiang Z, Gui Y, Gui Y, et al: MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma. Mol Med Rep 16: 9157-9164, 2017.
APA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J. ... Lai, Y. (2017). MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma. Molecular Medicine Reports, 16, 9157-9164. https://doi.org/10.3892/mmr.2017.7705
MLA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16.6 (2017): 9157-9164.
Chicago
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 9157-9164. https://doi.org/10.3892/mmr.2017.7705
Copy and paste a formatted citation
x
Spandidos Publications style
Jin L, Li Y, Nie L, He T, Hu J, Liu J, Chen M, Shi M, Jiang Z, Gui Y, Gui Y, et al: MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma. Mol Med Rep 16: 9157-9164, 2017.
APA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J. ... Lai, Y. (2017). MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma. Molecular Medicine Reports, 16, 9157-9164. https://doi.org/10.3892/mmr.2017.7705
MLA
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16.6 (2017): 9157-9164.
Chicago
Jin, L., Li, Y., Nie, L., He, T., Hu, J., Liu, J., Chen, M., Shi, M., Jiang, Z., Gui, Y., Yang, S., Lai, Y."MicroRNA‑24‑2 is associated with cell proliferation, invasion, migration and apoptosis in renal cell carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 9157-9164. https://doi.org/10.3892/mmr.2017.7705
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