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Article

Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction

  • Authors:
    • Huankai Yao
    • Zhenhai Yuan
    • Guangming Wei
    • Chun Chen
    • Jingyu Duan
    • Yan Li
    • Yunru Wang
    • Chunping Zhang
    • Yuanhua Liu
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy and Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Shandong Academy of Pharmaceutical Sciences, Jinan, Shandong 250101, P.R. China, Department of Pharmacy, Taian City Central Hospital, Taian, Shandong 271000, P.R. China
  • Pages: 9197-9202
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7712
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Abstract

Cerebral ischemia and following reperfusion affects many people worldwide. To discover efficient therapeutic approaches, numerous natural products have been investigated. The current study investigated the protective effects of thevetiaflavone, a natural flavonoid obtained from Wikstroemia indica, and the associated mechanisms using PC12 cells induced by oxygen and glucose deprivation. As a result, thevetiaflavone improves cell viability and suppresses the leakage of lactate dehydrogenase from the cytoplasm. Further investigation of the mechanisms demonstrated that thevetiaflavone decreases overproduction of ROS and ameliorates ROS‑mediated mitochondrial dysfunction, including collapse of mitochondrial membrane potential and mitochondrial permeability transition pore opening. Thevetiaflavone reduces the intracellular Ca2+ level, which is closely associated with mitochondrial function and interplays with ROS. Furthermore, thevetiaflavone inhibits apoptosis in PC12 cells through upregulating the expression of Bcl‑2 and downregulating that of Bax and caspase‑3 in addition to increasing the activity of caspase‑3. These results further indicate the protective effects of thevetiaflavone in vivo and its application in the clinic.
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Copy and paste a formatted citation
Spandidos Publications style
Yao H, Yuan Z, Wei G, Chen C, Duan J, Li Y, Wang Y, Zhang C and Liu Y: Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction. Mol Med Rep 16: 9197-9202, 2017.
APA
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y. ... Liu, Y. (2017). Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction. Molecular Medicine Reports, 16, 9197-9202. https://doi.org/10.3892/mmr.2017.7712
MLA
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y., Wang, Y., Zhang, C., Liu, Y."Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction". Molecular Medicine Reports 16.6 (2017): 9197-9202.
Chicago
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y., Wang, Y., Zhang, C., Liu, Y."Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction". Molecular Medicine Reports 16, no. 6 (2017): 9197-9202. https://doi.org/10.3892/mmr.2017.7712
Copy and paste a formatted citation
x
Spandidos Publications style
Yao H, Yuan Z, Wei G, Chen C, Duan J, Li Y, Wang Y, Zhang C and Liu Y: Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction. Mol Med Rep 16: 9197-9202, 2017.
APA
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y. ... Liu, Y. (2017). Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction. Molecular Medicine Reports, 16, 9197-9202. https://doi.org/10.3892/mmr.2017.7712
MLA
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y., Wang, Y., Zhang, C., Liu, Y."Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction". Molecular Medicine Reports 16.6 (2017): 9197-9202.
Chicago
Yao, H., Yuan, Z., Wei, G., Chen, C., Duan, J., Li, Y., Wang, Y., Zhang, C., Liu, Y."Thevetiaflavone from Wikstroemia indica ameliorates PC12 cells injury induced by OGD/R via improving ROS‑mediated mitochondrial dysfunction". Molecular Medicine Reports 16, no. 6 (2017): 9197-9202. https://doi.org/10.3892/mmr.2017.7712
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