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December-2017 Volume 16 Issue 6

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Article

SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro

  • Authors:
    • Chunting Wu
    • Jiahui Zhao
    • Guangfa Zhu
    • Yan Huang
    • Liyan Jin
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China, Department of Urology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China
  • Pages: 9060-9066
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7715
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Abstract

Acute lung injury (ALI) is a condition of acute respiratory failure, characterized by diffuse pulmonary infiltrates and severe hypoxemia. During ALI, the acute phase of inflammation induces the recruitment of activated inflammatory cells, including macrophages and lymphocytes, to the damaged lesions. Nuclear factor (NF)‑κB is a key protein in many signal transduction pathways, over‑activation of which is followed by an approach of inflammation cells and release of pre‑inflammation cytokines. The aim of the present study was to explore the effect of NF‑κB P65 siRNA retroviruses on the activation of NF‑κB signaling pathway and release of pro‑inflammatory cytokines in THP‑1 cells. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting were used to detect the NF‑κB p65 mRNA and protein expression at different times in THP‑1 cells infected by p65 siRNA retroviruses. The results revealed that p65 siRNA retroviruses could significantly inhibit the expression levels of NF‑κB p65 mRNA and protein at different times. In addition, to further investigate the effect of p65 siRNA retroviruses on the pro‑inflammatory cytokines release stimulated by LPS, the expression of IL‑1β in THP‑1 cells and TNF‑α in THP‑1/M cells was also detected using RT‑qPCR and ELISA. As a result, the level of released proinflammatory cytokine interleukin‑1β and tumor necrosis factor‑α stimulated was significantly inhibited at different times infected by p65 siRNA retroviruses, while increased at different times infected by siControl retroviruses in THP‑1 and THP‑1/M cells stimulated by LPS. In summary, the present study demonstrated that p65 siRNA retroviruses could suppress the activation of NF‑κB signal pathway and release of pro‑inflammatory cytokines in THP‑1 cells which provided a clinically plausible method to inhibit the inflammation for ALI/ARDS utilizing RNA interference technology.
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View References

1 

Wheeler AP and Bernard GR: Acute lung injury and the acute respiratory distress syndrome: A clinical review. Lancet. 369:1553–1564. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE and Clancy JP: Matrix metalloproteinase activity in pediatric acute lung injury. Int J Med Sci. 6:9–17. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Al-Harbi NO, Imam F, Al-Harbi MM, Ansari MA, Zoheir KM, Korashy HM, Sayed-Ahmed MM, Attia SM, Shabanah OA and Ahmad SF: Dexamethasone attenuates LPS-induced acute lung injury through inhibition of NF-κB, COX-2, and Pro-inflammatory mediators. Immunol Invest. 45:349–369. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Wu CT, Zhu GF, Zhao JH, Zhang XF, Jin LY and Yan SF: Effect of retroviral vector mediating RNA interference on the release of tumor necrosis factor-alpha in lipopolysaccharide-stimulating macrophage. Zhonghua Yi Xue Za Zhi. 90:1283–1287. 2010.(In Chinese). PubMed/NCBI

5 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Zhang X, Sun CY, Zhang YB, Guo HZ, Feng XX, Peng SZ, Yuan J, Zheng RB, Chen WP, Su ZR and Huang XD: Kegan Liyan oral liquid ameliorates lipopolysaccharide-induced acute lung injury through inhibition of TLR4-mediated NF-κB signaling pathway and MMP-9 expression. J Ethnopharmacol. 186:91–102. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Kim YS, Hwang JW, Jang JH, Son S, Seo IB, Jeong JH, Kim EH, Moon SH, Jeon BT and Park PJ: Trapa japonica pericarp extract reduces LPS-induced inflammation in macrophages and acute lung injury in mice. Molecules. 21:3922016. View Article : Google Scholar : PubMed/NCBI

8 

Sun CY, Xu LQ, Zhang ZB, Chen CH, Huang YZ, Su ZQ, Guo HZ, Chen XY, Zhang X, Liu YH, et al: Protective effects of pogostone against LPS-induced acute lung injury in mice via regulation of Keap1-Nrf2/NF-κB signaling pathways. Int Immunopharmacol. 32:55–61. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Qiu J, Yu L, Zhang X, Wu Q, Wang D, Wang X, Xia C and Feng H: Asiaticoside attenuates lipopolysaccharide-induced acute lung injury via down-regulation of NF-κB signaling pathway. Int Immunopharmacol. 26:181–187. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Wang J, Liu YT, Xiao L, Zhu L, Wang Q and Yan T: Anti-inflammatory effects of apigenin in lipopolysaccharide-induced inflammatory in acute lung injury by suppressing COX-2 and NF-κB pathway. Inflammation. 37:2085–2090. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Yang L, Li D, Zhuo Y, Zhang S, Wang X and Gao H: Protective role of liriodendrin in sepsis-induced acute lung injury. Inflammation. 39:1805–1813. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Lucas SM, Rothwell NJ and Gibson RM: The role of inflammation in CNS injury and disease. Br J Pharmacol. 147 Suppl 1:S232–S240. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Perera M Nilupul, Ma HK, Arakawa S, Howells DW, Markus R, Rowe CC and Donnan GA: Inflammation following stroke. J Clin Neurosci. 13:1–8. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Liang D, Sun Y, Shen Y, Li F, Song X, Zhou E, Zhao F, Liu Z, Fu Y, Guo M, et al: Shikonin exerts anti-inflammatory effects in a murine model of lipopolysaccharide-induced acute lung injury by inhibiting the nuclear factor-kappaB signaling pathway. Int Immunopharmacol. 16:475–480. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Zhu T, Wang DX, Zhang W, Liao XQ, Guan X, Bo H, Sun JY, Huang NW, He J, Zhang YK, et al: Andrographolide protects against LPS-induced acute lung injury by inactivation of NF-κB. PLoS One. 8:e564072013. View Article : Google Scholar : PubMed/NCBI

16 

Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:806–811. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K and Tuschl T: Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494–498. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Greenlee-Wacker MC: Clearance of apoptotic neutrophils and resolution of inflammation. Immunol Rev. 273:357–370. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu C, Zhao J, Zhu G, Huang Y and Jin L: SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Mol Med Rep 16: 9060-9066, 2017.
APA
Wu, C., Zhao, J., Zhu, G., Huang, Y., & Jin, L. (2017). SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Molecular Medicine Reports, 16, 9060-9066. https://doi.org/10.3892/mmr.2017.7715
MLA
Wu, C., Zhao, J., Zhu, G., Huang, Y., Jin, L."SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro". Molecular Medicine Reports 16.6 (2017): 9060-9066.
Chicago
Wu, C., Zhao, J., Zhu, G., Huang, Y., Jin, L."SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro". Molecular Medicine Reports 16, no. 6 (2017): 9060-9066. https://doi.org/10.3892/mmr.2017.7715
Copy and paste a formatted citation
x
Spandidos Publications style
Wu C, Zhao J, Zhu G, Huang Y and Jin L: SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Mol Med Rep 16: 9060-9066, 2017.
APA
Wu, C., Zhao, J., Zhu, G., Huang, Y., & Jin, L. (2017). SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Molecular Medicine Reports, 16, 9060-9066. https://doi.org/10.3892/mmr.2017.7715
MLA
Wu, C., Zhao, J., Zhu, G., Huang, Y., Jin, L."SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro". Molecular Medicine Reports 16.6 (2017): 9060-9066.
Chicago
Wu, C., Zhao, J., Zhu, G., Huang, Y., Jin, L."SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro". Molecular Medicine Reports 16, no. 6 (2017): 9060-9066. https://doi.org/10.3892/mmr.2017.7715
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