Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration

  • Authors:
    • Fei Zou
    • Zhiwen Ding
    • Jianyuan Jiang
    • Feizhou Lu
    • Xinlei Xia
    • Xiaosheng Ma
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
  • Pages: 9173-9180
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7718
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Circular RNAs (circRNAs) are relatively recently identified noncoding RNAs that are ubiquitously expressed in human tissues and serve key functions in regulating gene expression. However, few studies have focused on human intervertebral disc degeneration (IDD) circRNAs, and the potential role of circRNAs in IDD has not been described in detail. In the present study, circRNA expression data was downloaded from the Gene Expression Omnibus database, and circRNAs in the human intervertebral disc were classified according to their length, indicating their uniform distribution of circRNAs of different lengths. Gene Ontology analysis was performed, which indicated that the differentially expressed circRNAs were mainly produced as a result of catalytic activity and from binding genes in the molecular function category, cell part genes in the cellular component category, and cellular and metabolic process genes in the biological process category. Classification analysis divided the circRNAs host genes into 16 classes; with nucleic acid binding genes ranked as the most common host gene type in IDD tissue. Pathway analysis indicated that >15 signaling pathways may serve different roles in IDD, and Wnt signaling, gonadotropin‑releasing hormone receptor and integrin signaling pathways may serve important roles. Using co‑expression analysis, 76 differentially expressed circRNAs and host gene pairs were identified, which were divided into four groups: CircRNAs and their host genes downregulated; circRNAs downregulated and host genes upregulated; circRNAs and their host genes upregulated; and circRNAs upregulated and host genes downregulated. Finally, hsa_circ_0008305 upregulation and hsa_circ_0041946 downregulation were validated in IDD using reverse transcription‑quantitative polymerase chain reaction. In conclusion, the findings of the present study may shed light on the potential roles of circRNAs in IDD and the possibility for their use in the diagnosis and clinical treatment of IDD in the future.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Benjamin RW and Xuejun W: Intervertebral disc (IVD): Structure, degeneration, repair and regeneration. Mater Sci Engineer C. 32:61–77. 2012. View Article : Google Scholar

2 

Deshpande BR, Katz JN, Solomon DH, Yelin EH, Hunter DJ, Messier SP, Suter LG and Losina E: Number of persons with symptomatic knee osteoarthritis in the US: Impact of race and ethnicity, Age, Sex, and Obesity. Arthritis Care Res (Hoboken). 68:1743–1750. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Song YQ, Karasugi T, Cheung KM, Chiba K, Ho DW, Miyake A, Kao PY, Sze KL, Yee A, Takahashi A, et al: Lumbar disc degeneration is linked to a carbohydrate sulfotransferase 3 variant. J Clin Invest. 123:4909–4917. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Gruber HE, Hoelscher GL, Ingram JA, Bethea S, Zinchenko N and Hanley EN Jr: Variations in aggrecan localization and gene expression patterns characterize increasing stages of human intervertebral disk degeneration. Exp Mol Pathol. 91:534–539. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Gruber HE, Hoelscher GL, Ingram JA and Hanley EN Jr: Genome-wide analysis of pain-, nerve- and neurotrophin-related gene expression in the degenerating human annulus. Mol Pain. 8:632012. View Article : Google Scholar : PubMed/NCBI

6 

Sun Z, Wang HQ, Liu ZH, Chang L, Chen YF, Zhang YZ, Zhang WL, Gao Y, Wan ZY, Che L, et al: Down-regulated CK8 expression in human intervertebral disc degeneration. Int J Med Sci. 10:948–956. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Sun Z, Guo YS, Yan SJ, Wan ZY, Gao B, Wang L, Liu ZH, Gao Y, Samartzis D, Lan LF, et al: CK8 phosphorylation induced by compressive loads underlies the downregulation of CK8 in human disc degeneration by activating protein kinase C. Lab Invest. 93:1323–1330. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Brosnan CA and Voinnet O: The long and the short of noncoding RNAs. Curr Opin Cell Biol. 21:416–425. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Batista PJ and Chang HY: Long noncoding RNAs: Cellular address codes in development and disease. Cell. 152:1298–1307. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Jarroux J, Morillon A and Pinskaya M: History, discovery, and classification of lncRNAs. Adv Exp Med Biol. 1008:1–46. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Maxmen A: RNA: The genome's rising stars. Nature. 496:127–129. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Brown CJ, Ballabio A, Rupert JL, Lafreniere RG, Grompe M, Tonlorenzi R and Willard HF: A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature. 349:38–44. 1991. View Article : Google Scholar : PubMed/NCBI

13 

Bartolomei MS, Zemel S and Tilghman SM: Parental imprinting of the mouse H19 gene. Nature. 351:153–155. 1991. View Article : Google Scholar : PubMed/NCBI

14 

Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, Marzluff WF and Sharpless NE: Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 19:141–157. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Memczak S, Jens M, Elefsinioti A, Torti F, Krueger J, Rybak A, Maier L, Mackowiak SD, Gregersen LH, Munschauer M, et al: Circular RNAs are a large class of animal RNAs with regulatory potency. Nature. 495:333–338. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Greene J, Baird AM, Brady L, Lim M, Gray SG, McDermott R and Finn SP: Circular RNAs: Biogenesis, Function and role in human diseases. Front Mol Biosci. 4:382017. View Article : Google Scholar : PubMed/NCBI

17 

Ashwal-Fluss R, Meyer M, Pamudurti NR, Ivanov A, Bartok O, Hanan M, Evantal N, Memczak S, Rajewsky N and Kadener S: circRNA biogenesis competes with pre-mRNA splicing. Mol Cell. 56:55–66. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Tay Y, Rinn J and Pandolfi PP: The multilayered complexity of ceRNA crosstalk and competition. Nature. 505:344–352. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Lan PH, Liu ZH, Pei YJ, Wu ZG, Yu Y, Yang YF, Liu X, Che L, Ma CJ, Xie YK, et al: Landscape of RNAs in human lumbar disc degeneration. Oncotarget. 7:63166–63176. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Pfirrmann CW, Metzdorf A, Zanetti M, Hodler J and Boos N: Magnetic resonance classification of lumbar intervertebral disc degeneration. Spine (Phila Pa 1976). 26:1873–1878. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Reno C, Marchuk L, Sciore P, Frank CB and Hart DA: Rapid isolation of total RNA from small samples of hypocellular, dense connective tissues. Biotechniques. 22:1082–1086. 1997.PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Huang S, Yang B, Chen BJ, Bliim N, Ueberham U, Arendt T and Janitz M: The emerging role of circular RNAs in transcriptome regulation. Genomics. Jun 26–2017.(Epub ahead of print). View Article : Google Scholar

25 

Xia S, Feng J, Lei L, Hu J, Xia L, Wang J, Xiang Y, Liu L, Zhong S, Han L and He C: Comprehensive characterization of tissue-specific circular RNAs in the human and mouse genomes. Brief Bioinform: Agu. 20–2016.(Epub ahead of print). View Article : Google Scholar

26 

Werfel S, Nothjunge S, Schwarzmayr T, Strom TM, Meitinger T and Engelhardt S: Characterization of circular RNAs in human, mouse and rat hearts. J Mol Cell Cardiol. 98:103–107. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ and Hoyland JA: Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 35:652–655. 2017. View Article : Google Scholar

28 

Wang J, Markova D, Anderson DG, Zheng Z, Shapiro IM and Risbud MV: TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc. J Biol Chem. 286:39738–39749. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Jia H, Ma J, Lv J, Ma X, Xu W, Yang Y, Tian A, Wang Y, Sun L, Xu L, et al: Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity. Sci Rep. 6:275212016. View Article : Google Scholar : PubMed/NCBI

30 

Xie H, Jing Y, Xia J, Wang X, You C and Yan J: Aquaporin 3 protects against lumbar intervertebral disc degeneration via the Wnt/β-catenin pathway. Int J Mol Med. 37:859–864. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Zhou N, Lin X, Dong W, Huang W, Jiang W, Lin L, Qiu Q, Zhang X, Shen J, Song Z, et al: SIRT1 alleviates senescence of degenerative human intervertebral disc cartilage endo-plate cells via the p53/p21 pathway. Sci Rep. 6:226282016. View Article : Google Scholar : PubMed/NCBI

32 

Wang Z, Wang G, Zhu X, Geng D and Yang H: Interleukin-2 is upregulated in patients with a prolapsed lumbar intervertebral disc and modulates cell proliferation, apoptosis and extracellular matrix metabolism of human nucleus pulposus cells. Exp Ther Med. 10:2437–2443. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Hiyama A, Sakai D, Tanaka M, Arai F, Nakajima D, Ab K and Mochida J: The relationship between the Wnt/β-catenin and TGF-β/BMP signals in the intervertebral disc cell. J Cell Physiol. 226:1139–1148. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Cortes-López M and Miura P: Emerging functions of circular RNAs. Yale J Biol Med. 89:527–537. 2016.PubMed/NCBI

35 

Kaneyama S, Nishida K, Takada T, Suzuki T, Shimomura T, Maeno K, Kurosaka M and Doita M: Fas ligand expression on human nucleus pulposus cells decreases with disc degeneration processes. J Orthop Sci. 13:130–135. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Masuda K, Oegema TR Jr and An HS: Growth factors and treatment of intervertebral disc degeneration. Spine (Phila Pa 1976). 29:2757–2769. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Garratt AN, Britsch S and Birchmeier C: Neuregulin, a factor with many functions in the life of a schwann cell. Bioessays. 22:987–996. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Sedlmeier G and Sleeman JP: Extracellular regulation of BMP signaling: Welcome to the matrix. Biochem Soc Trans. 45:173–181. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zou F, Ding Z, Jiang J, Lu F, Xia X and Ma X: Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration. Mol Med Rep 16: 9173-9180, 2017.
APA
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., & Ma, X. (2017). Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration. Molecular Medicine Reports, 16, 9173-9180. https://doi.org/10.3892/mmr.2017.7718
MLA
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., Ma, X."Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration". Molecular Medicine Reports 16.6 (2017): 9173-9180.
Chicago
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., Ma, X."Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration". Molecular Medicine Reports 16, no. 6 (2017): 9173-9180. https://doi.org/10.3892/mmr.2017.7718
Copy and paste a formatted citation
x
Spandidos Publications style
Zou F, Ding Z, Jiang J, Lu F, Xia X and Ma X: Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration. Mol Med Rep 16: 9173-9180, 2017.
APA
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., & Ma, X. (2017). Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration. Molecular Medicine Reports, 16, 9173-9180. https://doi.org/10.3892/mmr.2017.7718
MLA
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., Ma, X."Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration". Molecular Medicine Reports 16.6 (2017): 9173-9180.
Chicago
Zou, F., Ding, Z., Jiang, J., Lu, F., Xia, X., Ma, X."Confirmation and preliminary analysis of circRNAs potentially involved in human intervertebral disc degeneration". Molecular Medicine Reports 16, no. 6 (2017): 9173-9180. https://doi.org/10.3892/mmr.2017.7718
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team