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Article

Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9

  • Authors:
    • Ran Lv
    • Qi Bao
    • Yan Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China, Department of Obstetrics and Gynecology, Yicheng District Hospital of TCM, Shandong Academy of Chinese Medicine, Zaozhuang, Shandong 277300, P.R. China
  • Pages: 9111-9119
    |
    Published online on: October 4, 2017
       https://doi.org/10.3892/mmr.2017.7719
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Abstract

Generally considered as a potent pro‑inflammatory signal, β‑galactosidelectin suppresses T cell receptor activation, can both promote and inhibit integrin‑mediated adhesion and is required in nuclear pre‑mRNA splicing. Galectin‑9 (Gal‑9), a member of β‑galactoside lectin, is involved many processes of T cell‑mediated diseases (such as autoimmune diseases and asthma) and immunomodulation of macrophages. Macrophages are involved in the occurrence of inflammation, development and digestion and other stages. At different stages of the inflammatory response, macrophages exhibit different phenotypes, but mainly two subtypes, classically (M1) or alternatively (M2) polarization. The purpose of this work is to investigate the effect of overexpression or knockdown of Gal‑9 on the macrophage polarization. Macrophage polarization was detected by flow cytometric profiling of secreted cytokines and specific surface markers expression, including nitric oxide synthase 2 (NOS2) and mannose receptor 1 (CD206). Protein and mRNA expression levels of TNF‑α, TGF‑β, IL‑6, IL‑10, NF‑κB, signal transducer and activator of transcription (Stat)1 and Stat3 were determined by ELISA, western blot analysis or qRT‑PCR. Our results implied that differentiation of the mouse macrophage line RAW264.7 into M1‑type and M2‑type macrophages is followed by marked variations of Gal‑9 expression. Furthermore, its overexpression and secretion are tightly associated with M2‑type macrophages, whereas its downregulation promotes macrophages to polarize into M1‑type macrophages, which confirmed by elevated CD206 and NOS2, respectively. In response to the changes of Gal‑9 expression, cytokines, transcription factors and regulators, including TNF‑α, IL‑6, NF‑κB, Stat1, TGF‑β, IL‑10, and Stat3, were tightly regulated and significantly associated with classically and alternatively activated macrophages. Consistent with characteristics of M1‑type macrophages, the transcriptional or translational expression levels or activity of TNF‑α, IL‑6, Stat1 and NF‑κB were markedly increased with knockdown of Gal‑9 in macrophages. By contrast, the expression levels or activity of TGF‑β, IL‑10 and Stat3 were clearly elevated in macrophages with Gal‑9 overexpression, which is closely related with M2‑type macrophages. Specific expression and secretion patterns of cytokines, transcription factors and regulators in M1‑type and M2‑type macrophages contribute to better understanding the role of Gal‑9 in regulation in macrophages. This study provides a new insight that Gal‑9 may be a new immunomodulatory target for macrophages.
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1 

Martinez FO, Sica A, Mantovani A and Locati M: Macrophage activation and polarization. Front Biosci. 13:453–461. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Mantovani A, Sozzani S, Locati M, Allavena P and Sica A: Macrophage polarization: Tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol. 23:549–555. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Wang C, Liu X, Liu Y, Zhang Q, Yao Z, Huang B, Zhang P, Li N and Cao X: Zinc finger protein 64 promotes Toll-like receptor-triggered proinflammatory and type I interferon production in macrophages by enhancing p65 subunit activation. J Biol Chem. 288:24600–24608. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Mantovani A, Sica A and Locati M: New vistas on macrophage differentiation and activation. Eur J Immunol. 37:14–16. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Macatonia SE, Hsieh CS, Murphy KM and O'Garra A: Dendritic cells and macrophages are required for Th1 development of CD4+ T cells from alpha beta TCR transgenic mice: IL-12 substitution for macrophages to stimulate IFN-gamma production is IFN-gamma-dependent. Int Immunol. 5:1119–1128. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Puddu P, Fantuzzi L, Borghi P, Varano B, Rainaldi G, Guillemard E, Malorni W, Nicaise P, Wolf SF, Belardelli F and Gessani S: IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages. J Immunol. 159:3490–3497. 1997.PubMed/NCBI

7 

Bashir S, Sharma Y, Elahi A and Khan F: Macrophage polarization: The link between inflammation and related diseases. Inflamm Res. 65:1–11. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Tymoszuk P, Charoentong P, Hackl H, Spilka R, Müller-Holzner E, Trajanoski Z, Obrist P, Revillion F, Peyrat JP, Fiegl H and Doppler W: High STAT1 mRNA levels but not its tyrosine phosphorylation are associated with macrophage infiltration and bad prognosis in breast cancer. BMC Cancer. 14:2572014. View Article : Google Scholar : PubMed/NCBI

9 

Satoh T, Takeuchi O, Vandenbon A, Yasuda K, Tanaka Y, Kumagai Y, Miyake T, Matsushita K, Okazaki T, Saitoh T, et al: The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection. Nat Immunol. 11:936–944. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Tamura M, Watanabe T, Igarashi T, Takeuchi T, Kasai K and Arata Y: Crosslinking of Cys-mutated human galectin-1 to the model glycoprotein ligands asialofetuin and laminin by using a photoactivatable bifunctional reagent. Biol Pharm Bull. 37:877–882. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Vicuña L, Pardo E, Curkovic C, Döger R, Oyanadel C, Metz C, Massardo L, González A and Soza A: Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients. Biol Res. 46:275–280. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Wijesundera KK, Izawa T, Tennakoon AH, Murakami H, Golbar HM, Katou-Ichikawa C, Tanaka M, Kuwamura M and Yamate J: M1- and M2-macrophage polarization in rat liver cirrhosis induced by thioacetamide (TAA), focusing on Iba1 and galectin-3. Exp Mol Pathol. 96:382–392. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Markovic B Simovic, Nikolic A, Gazdic M, Nurkovic J, Djordjevic I, Arsenijevic N, Stojkovic M, Lukic ML and Volarevic V: Pharmacological inhibition of Gal-3 in mesenchymal stem cells enhances their capacity to promote alternative activation of macrophages in dextran sulphate sodium-induced colitis. Stem Cells Int. 2016:26407462016.PubMed/NCBI

14 

Elahi S, Niki T, Hirashima M and Horton H: Galectin-9 binding to Tim-3 renders activated human CD4+ T cells less susceptible to HIV-1 infection. Blood. 119:4192–4204. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Wang F, He W, Yuan J, Wu K, Zhou H, Zhang W and Chen ZK: Activation of Tim-3-Galectin-9 pathway improves survival of fully allogeneic skin grafts. Transpl Immunol. 19:12–19. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Gooden MJ, Wiersma VR, Samplonius DF, Gerssen J, van Ginkel RJ, Nijman HW, Hirashima M, Niki T, Eggleton P, Helfrich W and Bremer E: Galectin-9 activates and expands human T-Helper 1 cells. PLoS One. 8:e656162013. View Article : Google Scholar : PubMed/NCBI

17 

Chabtini L, Mfarrej B, Mounayar M, Zhu B, Batal I, Dakle PJ, Smith BD, Boenisch O, Najafian N, Akiba H, et al: TIM-3 regulates innate immune cells to induce fetomaternal tolerance. J Immunol. 190:88–96. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Zhao Z, Jiang X, Kang C, Xiao Y, Hou C, Yu J, Wang R, Xiao H, Zhou T, Wen Z, et al: Blockade of the T cell immunoglobulin and mucin domain protein 3 pathway exacerbates sepsis-induced immune deviation and immunosuppression. Clin Exp Immunol. 178:279–291. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Li X, Chen Y, Liu X, Zhang J, He X, Teng G and Yu D: Tim3/Gal9 interactions between T cells and monocytes result in an immunosuppressive feedback loop that inhibits Th1 responses in osteosarcoma patients. Int Immunopharmacol. 44:153–159. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Wada J, Ota K, Kumar A, Wallner EI and Kanwar YS: Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin. J Clin Invest. 99:2452–2461. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Jiang X, Yu J, Shi Q, Xiao Y, Wang W, Chen G, Zhao Z, Wang R, Xiao H, Hou C, et al: Tim-3 promotes intestinal homeostasis in DSS colitis by inhibiting M1 polarization of macrophages. Clin Immunol. 160:328–335. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Mock BA, Krall MM, Byrd LG, Chin H, Barton CH, Charles I, Liew FY and Blackwell J: The inducible form of nitric oxide synthase (NOS2) isolated from murine macrophages maps near the nude mutation on mouse chromosome 11. Eur J Immunogenet. 21:231–238. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Liu HF, Zhang HJ, Hu QX, Liu XY, Wang ZQ, Fan JY, Zhan M and Chen FL: Altered polarization, morphology, and impaired innate immunity germane to resident peritoneal macrophages in mice with long-term type 2 diabetes. J Biomed Biotechnol. 2012:8670232012. View Article : Google Scholar : PubMed/NCBI

24 

Kienast K, Knorst M, Müller-Quernheim J and Ferlinz R: Modulation of IL-1beta, IL-6, IL-8, TNF-alpha, and TGF-beta secretions by alveolar macrophages under NO 2 exposure. Lung. 174:57–67. 1996.PubMed/NCBI

25 

Gu Y, Hu X, Liu C, Qv X and Xu C: Interleukin (IL)-17 promotes macrophages to produce IL-8, IL-6 and tumour necrosis factor-alpha in aplastic anaemia. Br J Haematol. 142:109–114. 2008. View Article : Google Scholar : PubMed/NCBI

26 

An SJ, Pae HO, Oh GS, Choi BM, Jeong S, Jang SI, Oh H, Kwon TO, Song CE and Chung HT: Inhibition of TNF-alpha, IL-1beta and IL-6 productions and NF-kappa B activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated from Catalpa ovata G. Don (Bignoniaceae). Int Immunopharmacol. 2:1173–1181. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Zhang Y, Cheng S, Zhang M, Zhen L, Pang D, Zhang Q and Li Z: High-infiltration of tumor-associated macrophages predicts unfavorable clinical outcome for node-negative breast cancer. PLoS One. 8:e761472013. View Article : Google Scholar : PubMed/NCBI

28 

Bastos KR, Alvarez JM, Marinho CR, Rizzo LV and Lima MR: Macrophages from IL-12p40-deficient mice have a bias toward the M2 activation profile. J Leukoc Biol. 71:271–278. 2002.PubMed/NCBI

29 

Jung SH, Hwang JH, Kim SE, Kim YK, Park HC and Lee HT: Human galectin-9 on the porcine cells affects the cytotoxic activity of M1-differentiated THP-1 cells through inducing a shift in M2-differentiated THP-1 cells. Xenotransplantation. 24:2017. View Article : Google Scholar

30 

Cao S, Zhang X, Edwards JP and Mosser DM: NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. J Biol Chem. 281:26041–26050. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Ohkubo N, Suzuki Y, Aoto M, Yamanouchi J, Hirakawa S, Yasukawa M and Mitsuda N: Accelerated destruction of erythrocytes in Tie2 promoter-driven STAT3 conditional knockout mice. Life Sci. 93:380–387. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Cramer LA, Nelson SL and Klemsz MJ: Synergistic induction of the Tap-1 gene by IFN-gamma and lipopolysaccharide in macrophages is regulated by STAT1. J Immunol. 165:3190–3197. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Choi JW, Kwon MJ, Kim IH, Kim YM, Lee MK and Nam TJ: Pyropia yezoensis glycoprotein promotes the M1 to M2 macrophage phenotypic switch via the STAT3 and STAT6 transcription factors. Int J Mol Med. 38:666–674. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Tyagi A, Singh RP, Ramasamy K, Raina K, Redente EF, Dwyer-Nield LD, Radcliffe RA, Malkinson AM and Agarwal R: Growth inhibition and regression of lung tumors by silibinin: Modulation of angiogenesis by macrophage-associated cytokines and nuclear factor-kappaB and signal transducers and activators of transcription 3. Cancer Prev Res (Phila). 2:74–83. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lv R, Bao Q and Li Y: Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9. Mol Med Rep 16: 9111-9119, 2017.
APA
Lv, R., Bao, Q., & Li, Y. (2017). Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9. Molecular Medicine Reports, 16, 9111-9119. https://doi.org/10.3892/mmr.2017.7719
MLA
Lv, R., Bao, Q., Li, Y."Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9". Molecular Medicine Reports 16.6 (2017): 9111-9119.
Chicago
Lv, R., Bao, Q., Li, Y."Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9". Molecular Medicine Reports 16, no. 6 (2017): 9111-9119. https://doi.org/10.3892/mmr.2017.7719
Copy and paste a formatted citation
x
Spandidos Publications style
Lv R, Bao Q and Li Y: Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9. Mol Med Rep 16: 9111-9119, 2017.
APA
Lv, R., Bao, Q., & Li, Y. (2017). Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9. Molecular Medicine Reports, 16, 9111-9119. https://doi.org/10.3892/mmr.2017.7719
MLA
Lv, R., Bao, Q., Li, Y."Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9". Molecular Medicine Reports 16.6 (2017): 9111-9119.
Chicago
Lv, R., Bao, Q., Li, Y."Regulation of M1‑type and M2‑type macrophage polarization in RAW264.7 cells by Galectin‑9". Molecular Medicine Reports 16, no. 6 (2017): 9111-9119. https://doi.org/10.3892/mmr.2017.7719
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