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Article Open Access

Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro

  • Authors:
    • Jian Gao
    • Feng Wan
    • Mo Tian
    • Yuanyuan Li
    • Yuxuan Li
    • Qiang Li
    • Jianping Zhang
    • Yongxue Wang
    • Xiang Huang
    • Lijuan Zhang
    • Yinchu Si
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, P.R. China, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, P.R. China, Department of Anatomy, Zhejiang Chinese Medicine University, Hangzhou, Zhejiang 310053, P.R. China, Massage Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, P.R. China, School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, P.R. China, Department of Traditional Chinese Medicine, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8875-8881
    |
    Published online on: October 6, 2017
       https://doi.org/10.3892/mmr.2017.7737
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Abstract

Ginsenoside‑Rg1, the main active component of Panax notoginseng, exhibits a number of pharmacological functions, including promoting protein synthesis in the brain, increasing the number of synapses, improving memory and promoting recovery of brain function following injury. The effect of ginsenoside‑Rg1 on proliferation and glial‑like‑directed differentiation in the cortical neural stem cells (NSCs) of embryonic rat brain was investigated. The present study used MTS assays to identify the optimum dose and window time of ginsenoside‑Rg1 administration to stimulate the proliferation of cortical NSCs in the rat embryonic tissue. The oxygen glucose deprivation (OGD) set‑up was used as a cell injury model. Immunofluorescent staining was used for identification of NSCs and subsequent observation of their proliferation and glial‑like directed differentiation. Nestin expression was the marker for the presence of NSCs among the cortical cells of embryonic rat brain. The optimum dose of ginsenoside‑Rg1 for proliferation of NSCs was 0.32 µg/ml. The optimum window time of 0.32 µg/ml ginsenoside‑Rg1 administration on proliferation of NSCs was 6 h. Ginsenoside‑Rg1 at 0.32 µg/ml concentration promoted incorporation of bromo‑2‑deoxyuridine, and expression of nestin and vimentin in primary and passaged NSCs, and NSCs following OGD. Ginsenoside‑Rg1 had a role in promoting proliferation and glial‑like‑directed differentiation of cortical NSCs. The plausible explanation for these responses is that ginsenoside‑Rg1 acts similarly to the growth factors to promote the proliferation and differentiation of NSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Gao J, Wan F, Tian M, Li Y, Li Y, Li Q, Zhang J, Wang Y, Huang X, Zhang L, Zhang L, et al: Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro. Mol Med Rep 16: 8875-8881, 2017.
APA
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q. ... Si, Y. (2017). Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro. Molecular Medicine Reports, 16, 8875-8881. https://doi.org/10.3892/mmr.2017.7737
MLA
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q., Zhang, J., Wang, Y., Huang, X., Zhang, L., Si, Y."Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro". Molecular Medicine Reports 16.6 (2017): 8875-8881.
Chicago
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q., Zhang, J., Wang, Y., Huang, X., Zhang, L., Si, Y."Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro". Molecular Medicine Reports 16, no. 6 (2017): 8875-8881. https://doi.org/10.3892/mmr.2017.7737
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J, Wan F, Tian M, Li Y, Li Y, Li Q, Zhang J, Wang Y, Huang X, Zhang L, Zhang L, et al: Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro. Mol Med Rep 16: 8875-8881, 2017.
APA
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q. ... Si, Y. (2017). Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro. Molecular Medicine Reports, 16, 8875-8881. https://doi.org/10.3892/mmr.2017.7737
MLA
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q., Zhang, J., Wang, Y., Huang, X., Zhang, L., Si, Y."Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro". Molecular Medicine Reports 16.6 (2017): 8875-8881.
Chicago
Gao, J., Wan, F., Tian, M., Li, Y., Li, Y., Li, Q., Zhang, J., Wang, Y., Huang, X., Zhang, L., Si, Y."Effects of ginsenoside‑Rg1 on the proliferation and glial‑like directed differentiation of embryonic rat cortical neural stem cells in vitro". Molecular Medicine Reports 16, no. 6 (2017): 8875-8881. https://doi.org/10.3892/mmr.2017.7737
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