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Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model

  • Authors:
    • Yuan Wang
    • Yong Yang
    • Jing‑Chuan Sun
    • Qin‑Jie Kong
    • Hai‑Bo Wang
    • Jian‑Gang Shi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai Changzheng Hospital, Shanghai 200003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9487-9493
    |
    Published online on: October 12, 2017
       https://doi.org/10.3892/mmr.2017.7774
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Abstract

The present study aimed to investigate the effect of the adenovirus‑mediated wild‑type p53‑induced protein phosphatase 1 (Wip1) gene on lumbar disc degeneration (LDD) in a rabbit model. Adult New Zealand white rabbits were used as experimental subjects. The rabbits were divided into LDD groups (groups A‑C of rabbit models of LDD) and control groups (groups D‑F of normal rabbits). The animals in groups A and D were injected with the Wip1 gene vector, those in groups B and E were injected with an empty vector, and those in groups C and F were injected with phosphate‑buffered saline. Type II collagen was detected using a streptavidin‑biotin complex immunohistochemistry method. Postoperative X‑ray imaging showed a significant increase in the recovery of rabbits from group A, compared with those from groups B and C. The nucleus pulposus proteoglycan content of the intervertebral discs (L2‑3, L3‑4 and L4‑5) of group A remained higher, compared with the content in groups B and C, and the values in groups B and C differed from those of groups E and F. At 3, 6 and 9 weeks post‑injection, the content of type II collagen of intervertebral discs (L2‑3, L3‑4 and L4‑5) in group A differed from groups B and C, and the values in groups A‑C remained lower, compared with those in groups D‑F. The Wip1 gene exhibited a therapeutic effect in the treatment of LDD.
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1 

Lee SY, Kim TH, Oh JK, Lee SJ and Park MS: Lumbar stenosis: A recent update by review of literature. Asian Spine J. 9:818–828. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Tian W and Qi H: Association between intervertebral disc degeneration and disturbances of blood supply to the vertebrae. Chin Med J (Engl). 123:239–243. 2010.PubMed/NCBI

3 

Eskola PJ, Lemmelä S, Kjaer P, Solovieva S, Männikkö M, Tommerup N, Lind-Thomsen A, Husgafvel-Pursiainen K, Cheung KM, Chan D, et al: Genetic association studies in lumbar disc degeneration: A systematic review. PLoS One. 7:e499952012. View Article : Google Scholar : PubMed/NCBI

4 

Natarajan RN and Andersson GB: Lumbar disc degeneration is an equally important risk factor as lumbar fusion for causing adjacent segment disc disease. J Orthop Res. 35:123–130. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Tschugg A, Michnacs F, Strowitzki M, Meisel HJ and Thomé C: A prospective multicenter phase I/II clinical trial to evaluate safety and efficacy of NOVOCART Disc plus autologous disc chondrocyte transplantation in the treatment of nucleotomized and degenerative lumbar disc to avoid secondary disease: Study protocol for a randomized controlled trial. Trials. 17:1082016. View Article : Google Scholar : PubMed/NCBI

6 

Phillips FM, Slosar PJ, Youssef JA, Andersson G and Papatheofanis F: Lumbar spine fusion for chronic low back pain due to degenerative disc disease: A systematic review. Spine (Phila Pa 1976). 38:E409–E422. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Woods BI, Vo N, Sowa G and Kang JD: Gene therapy for intervertebral disk degeneration. Orthop Clin North Am. 42:563–574. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Le Guezennec X and Bulavin DV: WIP1 phosphatase at the crossroads of cancer and aging. Trends Biochem Sci. 35:109–114. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Pechackova S, Burdova K, Benada J, Kleiblova P, Jenikova G and Macurek L: Inhibition of WIP1 phosphatase sensitizes breast cancer cells to genotoxic stress and to MDM2 antagonist nutlin-3. Oncotarget. 7:14458–14475. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Clausse V, Goloudina AR, Uyanik B, Kochetkova EY, Richaud S, Fedorova OA, Hammann A, Bardou M, Barlev NA, Garrido C and Demidov ON: Wee1 inhibition potentiates Wip1-dependent p53-negative tumor cell death during chemotherapy. Cell Death Dis. 7:e21952016. View Article : Google Scholar : PubMed/NCBI

11 

Wang L, Wang Z, Zhang F, Zhu R, Bi J, Wu J, Zhang H, Wu H, Kong W, Yu B and Yu X: Enhancing transgene expression from recombinant AAV8 vectors in different tissues using woodchuck hepatitis virus post-transcriptional regulatory element. Int J Med Sci. 13:286–291. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Ling C, Yin Z, Li J, Zhang D, Aslanidi G and Srivastava A: Strategies to generate high-titer, high-potency recombinant AAV3 serotype vectors. Mol Ther Methods Clin Dev. 3:160292016. View Article : Google Scholar : PubMed/NCBI

13 

Nicolson SC, Li C, Hirsch ML, Setola V and Samulski RJ: Identification and validation of small molecules that enhance recombinant Adeno-associated virus transduction following high throughput screen. J Virol. 90:7019–7031. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Orlans FB: Ethical decision making about animal experiments. Ethics Behav. 7:163–171. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Paul R, Haydon RC, Cheng H, Ishikawa A, Nenadovich N, Jiang W, Zhou L, Breyer B, Feng T, Gupta P, et al: Potential use of Sox9 gene therapy for intervertebral degenerative disc disease. Spine (Phila Pa 1976). 28:755–763. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Kim KS, Yoon ST, Li J, Park JS and Hutton WC: Disc degeneration in the rabbit: A biochemical and radiological comparison between four disc injury models. Spine (Phila Pa 1976). 30:33–37. 2005. View Article : Google Scholar : PubMed/NCBI

17 

de Schepper EI, Damen J, van Meurs JB, Ginai AZ, Popham M, Hofman A, Koes BW and Bierma-Zeinstra SM: The association between lumbar disc degeneration and low back pain: The influence of age, gender, and individual radiographic features. Spine (Phila Pa 1976). 35:531–536. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Liu Y, Yu T, Ma XX, Xiang HF, Hu YG and Chen BH: Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model. Exp Ther Med. 11:1399–1404. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Choi YS: Pathophysiology of degenerative disc disease. Asian Spine J. 3:39–44. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Ryu R, Techy F, Varadarajan R and Amirouche F: Effect of interbody fusion on the remaining discs of the lumbar spine in subjects with disc degeneration. Orthop Surg. 8:27–33. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Albert HB and Manniche C: Modic changes following lumbar disc herniation. Eur Spine J. 16:977–982. 2007. View Article : Google Scholar : PubMed/NCBI

22 

d'Adda di Fagagna F: Living on a break: Cellular senescence as a DNA-damage response. Nat Rev Cancer. 8:512–522. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Hat B, Kochańczyk M, Bogdał MN and Lipniacki T: Feedbacks, bifurcations, and cell fate decision-making in the p53 system. PLoS Comput Biol. 12:e10047872016. View Article : Google Scholar : PubMed/NCBI

24 

Mirzayans R, Andrais B, Scott A, Wang YW, Weiss RH and Murray D: Spontaneous γH2AX foci in human solid tumor-derived cell lines in relation to p21WAF1 and WIP1 expression. Int J Mol Sci. 16:11609–11628. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Iwamoto K, Hamada H, Eguchi Y and Okamoto M: Stochasticity of intranuclear biochemical reaction processes controls the final decision of cell fate associated with DNA damage. PLoS One. 9:e1013332014. View Article : Google Scholar : PubMed/NCBI

26 

Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ and Hoyland JA: Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 35:652–655. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Tang X, Jing L, Richardson WJ, Isaacs RE, Fitch RD, Brown CR, Erickson MM, Setton LA and Chen J: Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degeneration. J Orthop Res. 34:1316–1326. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Le Maitre CL, Freemont AJ and Hoyland JA: Accelerated cellular senescence in degenerate intervertebral discs: A possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther. 9:R452007. View Article : Google Scholar : PubMed/NCBI

29 

Sakai H, Fujigaki H, Mazur SJ and Appella E: Wild-type p53-induced phosphatase 1 (Wip1) forestalls cellular premature senescence at physiological oxygen levels by regulating DNA damage response signaling during DNA replication. Cell Cycle. 13:1015–1029. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Yang Y, Sun JC, Kong QJ, Wang HB and Shi JG: Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model. Mol Med Rep 16: 9487-9493, 2017.
APA
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., & Shi, J. (2017). Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model. Molecular Medicine Reports, 16, 9487-9493. https://doi.org/10.3892/mmr.2017.7774
MLA
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., Shi, J."Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model". Molecular Medicine Reports 16.6 (2017): 9487-9493.
Chicago
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., Shi, J."Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model". Molecular Medicine Reports 16, no. 6 (2017): 9487-9493. https://doi.org/10.3892/mmr.2017.7774
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yang Y, Sun JC, Kong QJ, Wang HB and Shi JG: Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model. Mol Med Rep 16: 9487-9493, 2017.
APA
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., & Shi, J. (2017). Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model. Molecular Medicine Reports, 16, 9487-9493. https://doi.org/10.3892/mmr.2017.7774
MLA
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., Shi, J."Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model". Molecular Medicine Reports 16.6 (2017): 9487-9493.
Chicago
Wang, Y., Yang, Y., Sun, J., Kong, Q., Wang, H., Shi, J."Effect of the adenovirus‑mediated Wip1 gene on lumbar intervertebral disc degeneration in a rabbit model". Molecular Medicine Reports 16, no. 6 (2017): 9487-9493. https://doi.org/10.3892/mmr.2017.7774
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