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Proteomic analysis of serum deprivation in tongue squamous cell carcinoma

  • Authors:
    • Junfeng Zhang
    • Wei Dong
    • Yufen Meng
    • Miao Jiang
    • Zhen Zhan
  • View Affiliations / Copyright

    Affiliations: Discipline of Chinese and Western Integrative Medicine, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, College of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9323-9330
    |
    Published online on: October 17, 2017
       https://doi.org/10.3892/mmr.2017.7807
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Abstract

The occurrence of tongue squamous cell carcinoma (TSCC) is closely correlated with serum components; however, the detailed mechanism remains to be fully elucidated. Proteomic analysis contributed to the discovery of potential biomarkers and provided an insight into TSCC at a molecular level. The present study investigated the effect of serum deprivation on the Tca‑8113 TSCC cell line through protein profiling using two‑dimensional gel electrophoresis and mass spectrometry, with the aim of improving TSCC diagnosis. The results showed that the Tca‑8113 cells maintained proliferative capacity and resisted apoptosis following serum deprivation. A total of 43 proteins were upregulated and 45 were downregulated following serum deprivation for 24 h, compared with untreated controls (0 h). The upregulated caspase-7, heat shock protein 27 and Annexin A1, and the downregulated peroxiredoxin‑6 and heat shock protein 70, were selected for verification using reverse transcription‑polymerase chain reaction analysis following serum deprivation for 16 h. The results indicated that reactive oxygen species may be important in serum deprivation‑induced oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Dong W, Meng Y, Jiang M and Zhan Z: Proteomic analysis of serum deprivation in tongue squamous cell carcinoma. Mol Med Rep 16: 9323-9330, 2017.
APA
Zhang, J., Dong, W., Meng, Y., Jiang, M., & Zhan, Z. (2017). Proteomic analysis of serum deprivation in tongue squamous cell carcinoma. Molecular Medicine Reports, 16, 9323-9330. https://doi.org/10.3892/mmr.2017.7807
MLA
Zhang, J., Dong, W., Meng, Y., Jiang, M., Zhan, Z."Proteomic analysis of serum deprivation in tongue squamous cell carcinoma". Molecular Medicine Reports 16.6 (2017): 9323-9330.
Chicago
Zhang, J., Dong, W., Meng, Y., Jiang, M., Zhan, Z."Proteomic analysis of serum deprivation in tongue squamous cell carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 9323-9330. https://doi.org/10.3892/mmr.2017.7807
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Dong W, Meng Y, Jiang M and Zhan Z: Proteomic analysis of serum deprivation in tongue squamous cell carcinoma. Mol Med Rep 16: 9323-9330, 2017.
APA
Zhang, J., Dong, W., Meng, Y., Jiang, M., & Zhan, Z. (2017). Proteomic analysis of serum deprivation in tongue squamous cell carcinoma. Molecular Medicine Reports, 16, 9323-9330. https://doi.org/10.3892/mmr.2017.7807
MLA
Zhang, J., Dong, W., Meng, Y., Jiang, M., Zhan, Z."Proteomic analysis of serum deprivation in tongue squamous cell carcinoma". Molecular Medicine Reports 16.6 (2017): 9323-9330.
Chicago
Zhang, J., Dong, W., Meng, Y., Jiang, M., Zhan, Z."Proteomic analysis of serum deprivation in tongue squamous cell carcinoma". Molecular Medicine Reports 16, no. 6 (2017): 9323-9330. https://doi.org/10.3892/mmr.2017.7807
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