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MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model

  • Authors:
    • Yulei Dong
    • Tao Li
    • Yulong Li
    • Shaoda Ren
    • Junfen Fan
    • Xisheng Weng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, P.R. China, Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Center of Tissue Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Institute of Basic Medical Sciences and School of Basic Medicine, Beijing 100005, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9331-9336
    |
    Published online on: October 17, 2017
       https://doi.org/10.3892/mmr.2017.7808
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Abstract

The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-density lipoprotein receptor-related protein 5 (LRP-5) expression was assessed by immunohistochemistry. The results demonstrated the incidence of osteonecrosis decreased in the miR-23a-3p inhibitor group compared with the miR-23a-3p mimic group (18.2% vs. 75%; P<0.05). The ratio of bone volume/total volume and trabecular thickness were significantly increased in the miR-23a-3p inhibitor group compared with the miR-23a mimic group. The expression level of LRP-5 was higher in the miR-23a-3p inhibitor group. The present study indicated that miR may provide a novel and alternative approach for understanding the mechanism underlying steroid-associated necrosis of the femoral head.
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Copy and paste a formatted citation
Spandidos Publications style
Dong Y, Li T, Li Y, Ren S, Fan J and Weng X: MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model. Mol Med Rep 16: 9331-9336, 2017.
APA
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., & Weng, X. (2017). MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model. Molecular Medicine Reports, 16, 9331-9336. https://doi.org/10.3892/mmr.2017.7808
MLA
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., Weng, X."MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model". Molecular Medicine Reports 16.6 (2017): 9331-9336.
Chicago
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., Weng, X."MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model". Molecular Medicine Reports 16, no. 6 (2017): 9331-9336. https://doi.org/10.3892/mmr.2017.7808
Copy and paste a formatted citation
x
Spandidos Publications style
Dong Y, Li T, Li Y, Ren S, Fan J and Weng X: MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model. Mol Med Rep 16: 9331-9336, 2017.
APA
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., & Weng, X. (2017). MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model. Molecular Medicine Reports, 16, 9331-9336. https://doi.org/10.3892/mmr.2017.7808
MLA
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., Weng, X."MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model". Molecular Medicine Reports 16.6 (2017): 9331-9336.
Chicago
Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., Weng, X."MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model". Molecular Medicine Reports 16, no. 6 (2017): 9331-9336. https://doi.org/10.3892/mmr.2017.7808
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