Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury

  • Authors:
    • Fujiang Cao
    • Tao Liu
    • Shiwei Sun
    • Shiqing Feng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9355-9360
    |
    Published online on: October 17, 2017
       https://doi.org/10.3892/mmr.2017.7816
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the role of microRNA (miR)‑99b‑5p in spinal cord injury (SCI). Reverse transcription‑quantitative polymerase chain reaction demonstrated that, compared with control mice, the expression levels of miR‑99b‑5p were upregulated in the mouse spinal cord following SCI. Mechanistic target of rapamycin (mTOR) was predicted to be the possible target of miR‑99b‑5p according to TargetScan and microrna databases. Dual‑luciferase reporter assay verified that miR‑99b‑5p was able to target mTOR. Furthermore, the results of an apoptosis analysis demonstrated that there were few apoptotic neurons in the control group, whereas SCI induced a significant increase in the number of apoptotic cells. Conversely, apoptosis was inhibited following transfection with a miR‑99b‑5p inhibitor. The effects of miR‑99b‑5p on neurite growth were also evaluated. The results of an immunofluorescence analysis indicated that neurite growth was normal in the control group, whereas SCI induced a reduction in neurite growth, which was rescued following transfection with a miR‑99b‑5p inhibitor. The protein expression levels of mTOR were detected in the three groups by western blotting. The results demonstrated that, compared with the control group, the protein expression levels of mTOR were significantly reduced in SCI neurons, whereas transfection with a miR‑99b‑5p inhibitor suppressed the SCI‑induced reduction of mTOR. In conclusion, treatment with a miR‑99b‑5p inhibitor may attenuate SCI‑induced harmful alterations in spinal cord neurons via the regulation of mTOR expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

W.H.O. Spinal Cord Injury Fact Sheet N. 384. 2013, http://www.who.int/mediacentre/factsheets/fs384/en/November 11–2013

2 

Dumont RJ, Okonkwo DO, Verma S, Hurlbert RJ, Boulos PT, Ellegala DB and Dumont AS: Acute spinal cord injury, part I: Pathophysiologic mechanisms. Clin Neuropharmacol. 24:254–264. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Sekhon LH and Fehlings MG: Epidemiology, demographics, and pathophysiology of acute spinal cord injury. Spine (Phila Pa 1976). 26(24 Suppl): S2–S12. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Devivo MJ: Epidemiology of traumatic spinal cord injury: Trends and future implications. Spinal Cord. 50:365–372. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Crowe MJ, Bresnahan JC, Shuman SL, Masters JN and Beattie MS: Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med. 3:73–76. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Fitch MT and Silver J: CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure. Exp Neurol. 209:294–301. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Fauad K and Tetzlaff W: Rehabilitative training and plasticity following spinal cord injury. Exp Neurol. 235:91–99. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Strickland ER, Hook MA, Balaraman S, Huie JR, Grau JW and Miranda RC: MicroRNA dysregulation following spinal cord contusion: Implications for neural plasticity and repair. Neuroscience. 186:146–160. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Gingras AC, Raught B and Sonenberg N: mTOR signaling to translation. Curr Top Microbiol Immunol. 279:169–197. 2004.PubMed/NCBI

10 

Hay N and Sonenberg N: Upstream and downstream of mTOR. Genes Dev. 18:1926–1945. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Jaworski J and Sheng M: The growing role of mTOR in neuronal development and plasticity. Mol Neurobiol. 34:205–219. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Abe N, Borson SH, Gambello MJ, Wang F and Cavalli V: Mammalian target of rapamycin (mTOR) activation increases axonal growth capacity of injured peripheral nerves. J Biol Chem. 285:28034–28043. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Park KK, Liu K, Hu Y, Smith PD, Wang C, Cai B, Xu B, Connolly L, Kramvis I, Sahin M and He Z: Promoting axon regeneration in the adult CNS by modulation of the PTEN/mTOR pathway. Science. 322:963–966. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Ambros V: microRNAs: Tiny regulators with great potential. Cell. 107:823–826. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Fineberg SK, Kosik KS and Davidson BL: MicroRNA potentiate neural development. Neuron. 64:303–309. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Genda Y, Arai M, Ishikawa M, Tanaka S, Okabe T and Sakamoto A: microRNA changes in the dorsal horn of the spinal cord of rats with chronic constriction injury: A TaqMan® low density array study. Int J Mol Med. 31:129–137. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Jee MK, Jung JS, Choi JI, Jang JA, Kang KS, Im YB and Kang SK: MicroRNA 486 is a potentially novel target for the treatment of spinal cord injury. Brain. 135:1237–1252. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Harkema SJ: Neural plasticity after human spinal cord injury: Application of locomotor training to the rehabilitation of walking. Neuroscientist. 7:455–468. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Ding Y, Kastin AJ and Pan W: Neural plasticity after spinal cord injury. Curr Pharm Des. 11:1441–1450. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Ziu M, Fletcher L, Rana S, Jimenez DF and Digicaylioglu M: Temporal differences in microRNA expression patterns in astrocytes and neurons after ischemic injury. PLoS One. 6:e147242011. View Article : Google Scholar : PubMed/NCBI

22 

Loohuis NF Olde, Kos A, Martens GJ, Van Bokhoven H, Kasri N Nadif and Aschrafi A: MicroRNA networks direct neuronal development and plasticity. Cell Mol Life Sci. 69:89–102. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Ye X, Luo H, Chen Y, Wu Q, Xiong Y, Zhu J, Dia Y, Wu Z, Miao J and Wan J: MicroRNAs 99b-5p/100-5p regulated by endoplasmic reticulum stress are involved in abeta-induced pathologies. Front Aging Neurosci. 7:2102015. View Article : Google Scholar : PubMed/NCBI

24 

Jin Y, Tymen SD, Chen D, Fang ZJ, Zhao Y, Dragas D, Dai Y, Marucha PT and Zhou X: MicroRNA-99 family targets AKT/mTOR signaling pathway in dermal wound healing. PLoS One. 8:e644342013. View Article : Google Scholar : PubMed/NCBI

25 

Edgerton VR, de Leon RD, Tillakaratne N, Recktenwald MR, Hodgson JA and Roy RR: Use dependent plasticity in spinal stepping and standing. Adv Neurol. 72:233–247. 1997.PubMed/NCBI

26 

De Leon RD, Hodgson JA, Roy RR and Edgerton VR: Retention of hindlimb stepping ability in adult spinal cats after the cessation of step training. J Neurophysiol. 81:85–94. 1999.PubMed/NCBI

27 

Dobkin BH: Activity-dependent learning contributes to motor recovery. Ann Neurol. 44:158–160. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Martinez M and Rossignol S: A dual spinal cord lesion paradigm to study spinal locomotor plasticity in the cat. Ann N Y Acad Sci. 1279:127–134. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Cao F, Liu T, Sun S and Feng S: The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury. Mol Med Rep 16: 9355-9360, 2017.
APA
Cao, F., Liu, T., Sun, S., & Feng, S. (2017). The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury. Molecular Medicine Reports, 16, 9355-9360. https://doi.org/10.3892/mmr.2017.7816
MLA
Cao, F., Liu, T., Sun, S., Feng, S."The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury". Molecular Medicine Reports 16.6 (2017): 9355-9360.
Chicago
Cao, F., Liu, T., Sun, S., Feng, S."The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury". Molecular Medicine Reports 16, no. 6 (2017): 9355-9360. https://doi.org/10.3892/mmr.2017.7816
Copy and paste a formatted citation
x
Spandidos Publications style
Cao F, Liu T, Sun S and Feng S: The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury. Mol Med Rep 16: 9355-9360, 2017.
APA
Cao, F., Liu, T., Sun, S., & Feng, S. (2017). The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury. Molecular Medicine Reports, 16, 9355-9360. https://doi.org/10.3892/mmr.2017.7816
MLA
Cao, F., Liu, T., Sun, S., Feng, S."The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury". Molecular Medicine Reports 16.6 (2017): 9355-9360.
Chicago
Cao, F., Liu, T., Sun, S., Feng, S."The role of the miR-99b-5p/mTOR signaling pathway in neuroregeneration in mice following spinal cord injury". Molecular Medicine Reports 16, no. 6 (2017): 9355-9360. https://doi.org/10.3892/mmr.2017.7816
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team