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Article Open Access

miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling

  • Authors:
    • Fengdi Cao
    • Jialin Zhan
    • Xufeng Chen
    • Kai Zhang
    • Renfa Lai
    • Zhiqiang Feng
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Ophthalmology, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Tianjin Key Laboratory of Ionic‑Molecular Function of Cardiovascular Disease, Department of Cardiology, Second Hospital of Tianjin Medical University, Tianjin Institute of Cardiology, Tianjin 300211, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9301-9308
    |
    Published online on: October 19, 2017
       https://doi.org/10.3892/mmr.2017.7821
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Abstract

The canonical Wnt/β‑catenin signaling is important in the differentiation of human mesenchymal stem cells into osteoblasts. Accumulating evidence suggests that the expression of β‑catenin is, in part, regulated by specific microRNAs (miRNAs). The aim of the present study was to investigate the putative roles of miRNAs in osteoblast differentiation. Polymerase chain reaction (PCR) arrays were used to identify miRNAs that were differentially expressed between differentiated and non‑differentiated periodontal ligament stem cells (PDLSCs), and reverse transcription‑quantitative PCR (RT‑qPCR) was used for validation. Since miR‑214 was revealed to be significantly downregulated during PDLSC differentiation, its function was further investigated via silencing and overexpression. In addition, osteogenic differentiation of PDLSCs was evaluated at 10 and 21 days following induction, using Alizarin red staining and RT‑qPCR analysis for mRNA expression levels of the osteogenic differentiation markers alkaline phosphatase (ALP), osteocalcin and bone sialoprotein. Furthermore, the potential target genes of miR‑214 were investigated using a dual‑luciferase reporter assay, RT‑qPCR and western blot analysis, whereas a TOPflash/FOPflash reporter plasmid system followed by a luciferase assay was used to examine the effects of miR‑214 on Wnt/β‑catenin signaling. The present results demonstrated that miR‑214 was significantly downregulated during the osteoblastic differentiation of PDLSCs. Notably, its overexpression inhibited PDLSC differentiation, whereas its knockdown promoted PDLSC differentiation, as revealed by alterations in mRNA expression of osteoblast‑specific genes and ALP. In addition, miR‑214 was demonstrated to directly interact with the 3'‑untranslated region of the β‑catenin gene CTNNB1, and suppressed Wnt/β‑catenin signaling through the inhibition of β‑catenin. The results of the present study suggested that miR‑214 may participate in the regulation of the Wnt/β‑catenin signaling pathway, and may have potential as a candidate target for the development of preventive or therapeutic agents for the treatment of patients with osteogenic disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Cao F, Zhan J, Chen X, Zhang K, Lai R and Feng Z: miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling. Mol Med Rep 16: 9301-9308, 2017.
APA
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., & Feng, Z. (2017). miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling. Molecular Medicine Reports, 16, 9301-9308. https://doi.org/10.3892/mmr.2017.7821
MLA
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., Feng, Z."miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling". Molecular Medicine Reports 16.6 (2017): 9301-9308.
Chicago
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., Feng, Z."miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling". Molecular Medicine Reports 16, no. 6 (2017): 9301-9308. https://doi.org/10.3892/mmr.2017.7821
Copy and paste a formatted citation
x
Spandidos Publications style
Cao F, Zhan J, Chen X, Zhang K, Lai R and Feng Z: miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling. Mol Med Rep 16: 9301-9308, 2017.
APA
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., & Feng, Z. (2017). miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling. Molecular Medicine Reports, 16, 9301-9308. https://doi.org/10.3892/mmr.2017.7821
MLA
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., Feng, Z."miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling". Molecular Medicine Reports 16.6 (2017): 9301-9308.
Chicago
Cao, F., Zhan, J., Chen, X., Zhang, K., Lai, R., Feng, Z."miR-214 promotes periodontal ligament stem cell osteoblastic differentiation by modulating Wnt/β‑catenin signaling". Molecular Medicine Reports 16, no. 6 (2017): 9301-9308. https://doi.org/10.3892/mmr.2017.7821
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