Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy

Yang,Yong‑Li; Guo,Shou‑Jun; Zhang,Zheng‑Xia; Zhang,Yong; Liu,Ang; Xie,Li‑Ling; Zheng,Yu‑Zhong

doi:10.3892/mmr.2017.8280

March-2018 Volume 17 Issue 3

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March-2018 Volume 17 Issue 3

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Article

Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy

Authors:
- Yong‑Li Yang
- Shou‑Jun Guo
- Zheng‑Xia Zhang
- Yong Zhang
- Ang Liu
- Li‑Ling Xie
- Yu‑Zhong Zheng
View Affiliations / Copyright

Affiliations: School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou, Guangdong 521041, P.R. China, Department of Biology, Shantou University, Shantou, Guangdong 515063, P.R. China
Pages: 3870-3876
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Published online on: December 14, 2017

https://doi.org/10.3892/mmr.2017.8280
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Abstract

A non-ionic water-soluble galactomannan, isolated from the seed endosperm of Crotalaria mucronata Desv., may be an ideal thickener to increase food viscosity. The present study hypothesized that the viscous property of Crotalaria galactomannan may be associated with its spatial structure. Therefore, the structure of Crotalaria galactomannan was elucidated using an atomic force microscope. The results of the present study demonstrated that the polysaccharide consisted of a D‑mannose backbone with D‑galactose branches, and the D‑mannose/D‑galactose ratio was 2.375:1. In the three‑dimensional structure of Crotalaria galactomannan, the helix was a common secondary structure, containing numerous ring structures of different sizes. In addition, multiple helixes may link together via hydrogen bonding and van der Waals forces, forming aggregations with small rings or spiral windings. The results of the present study indicated that the multiple‑branching construction of Crotalaria galactomannan may underlie its viscosity‑enhancing properties in the water phase.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Structural elucidation of galactomannan from seeds of Crotalaria mucronata Desv. by atomic force microscopy

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