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Article

miR-373 suppresses gastric cancer metastasis by downregulating vimentin

  • Authors:
    • Yinghong Shi
    • Hui Shi
    • Bin Zhang
    • Yongmin Yan
    • Xinye Han
    • Wenqian Jiang
    • Hui Qian
    • Wenrong Xu
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Laboratory Medicine of Jiangsu Province, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
  • Pages: 4027-4034
    |
    Published online on: December 18, 2017
       https://doi.org/10.3892/mmr.2017.8291
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Abstract

MicroRNA-373 (miR-373) has been reported to be an oncogene in a number of solid human tumors. However, the role of miR‑373 in gastric cancer has not been completely elucidated and the mechanisms remain unclear. In the present study, we compared miR‑373 expression between clinical gastric cancer tissues and paired non‑tumorous tissues by reverse transcription‑quantitative polymerase chain reaction. The impact of miR‑373 on proliferation, migration and invasion in gastric cancer cells was additionally investigated. Hsa‑miR‑373 mimics were applied to mimic the function of endogenous miR‑373. A colony formation assay and flow cytometry were performed to analyze the proliferation of gastric cancer cells. Wound healing and Transwell invasion assays were employed to detect the migratory and invasive abilities of gastric cancer cells. Western blotting was used to test the expression of epithelial‑mesenchymal transition‑associated proteins. The results demonstrated that the level of miR‑373 in gastric cancer was upregulated compared with paired non‑tumorous tissues. It was confirmed that miR‑373 inhibited the migration and invasion of the gastric cancer cell lines SGC‑7901 and HGC‑27 by downregulating vimentin expression. The results of the present study demonstrated an oncogenic role of miR‑373 in the metastasis of human gastric cancer, and may provide a novel therapeutic strategy for gastric cancer.
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1 

Brenner H, Rothenbacher D and Arndt V: Epidemiology of stomach cancer. Methods Mol Biol. 472:467–477. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Desai AM, Pareek M, Nightingale PG and Fielding JW: Improving outcomes in gastric cancer over 20 years. Gastric Cancer. 7:196–201. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Crew KD and Neugut AI: Epidemiology of gastric cancer. World J Gastroenterol. 12:354–362. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Krol J, Loedige I and Filipowicz W: The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet. 11:597–610. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA and van Oudenaarden A: MicroRNAscan generate thresholds in target gene expression. Nat Genet. 43:854–859. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Pichinuk E, Broday L and Wreschner DH: Endogenous RNA cleavages at the ribosomal SRL site likely reflect miRNA mediated translational suppression. Biochem Biophys Res Commun. 414:706–711. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Wang M, Gu H, Qian H, Zhu W, Zhao C, Zhang X, Tao Y, Zhang L and Xu W: miR-17-5p/20a are important markers for gastric cancer and murine double minute 2 participates in their functional regulation. Eur J Cancer. 49:2010–2021. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Mitash N, Agnihotri S, Tiwari S, Agrawal V and Mandhani A: MicroRNA-21 could be a molecular marker to predict the recurrence of nonmuscle invasive bladder cancer. Indian J Urol. 33:283–290. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Jiao W, Leng X, Zhou Q, Wu Y, Sun L, Tan Y, Ni H, Dong X, Shen T, Liu Y and Li J: Different miR-21-3p isoforms and their different features in colorectal cancer. Int J Cancer. 141:2103–2111. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Song Y, Zuo Y, Qian XL, Chen ZP, Wang SK, Song L and Peng LP: Inhibition of MicroRNA-21-5p promotes the radiation sensitivity of non-small cell lung cancer through HMSH2. Cell Physiol Biochem. 43:1258–1272. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Motoyama K, Inoue H, Mimori K, Tanaka F, Kojima K, Uetake H, Sugihara K and Mori M: Clinicopathological and prognostic significance of PDCD4 and microRNA-21 in human gastric cancer. Int J Oncol. 36:1089–1095. 2010.PubMed/NCBI

13 

Wu Y, Song Y, Xiong Y, Wang X, Xu K, Han B, Bai Y, Li L, Zhang Y and Zhou L: MicroRNA-21 (Mir-21) promotes cell growth and invasion by repressing tumor suppressor PTEN in colorectal cancer. Cell Physiol Biochem. 43:945–958. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Zhang BG, Li JF, Yu BQ, Zhu ZG, Liu BY and Yan M: microRNA-21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN. Oncol Rep. 27:1019–1026. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Wang L, Qu J, Zhou L, Liao F and Wang J: MicroRNA-373 inhibits cell proliferation and invasion via targeting BRF2 in human non-small cell lung cancer A549 cell line. Cancer Res Treat. Oct 12–2017.(Epub ahead of print). View Article : Google Scholar :

16 

Li Y, Zhang D and Wang J: MicroRNA-373 promotes tumorigenesis of renal cell carcinoma in vitro and in vivo. Mol Med Rep Sep. 16:7048–7055. 2017. View Article : Google Scholar

17 

Hua Y, Chen H, Wang L, Wang F, Wang P, Ning Z, Li Y, Liu L, Chen Z and Meng Z: Low serum miR-373 predicts poor prognosis in patients with pancreatic cancer. Cancer Biomark. 20:95–100. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, et al: Human embryonic stem cells express a unique set of microRNAs. Dev Biol. 270:488–498. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Zhang X, Yan Z, Zhang J, Gong L, Li W, Cui J, Liu Y, Gao Z, Li J, Shen L and Lu Y: Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Ann Oncol. 22:2257–2266. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Yan Z, Xiong Y, Xu W, Li M, Cheng Y, Chen F, Ding S, Xu H and Zheng G: Identification of recurrence-related genes by integrating microRNA and gene expression profiling of gastriccancer. Int J Oncol. 41:2166–2174. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Zhang X, Li X, Tan Z, Liu X, Yang C, Ding X, Hu X, Zhou J, Xiang S, Zhou C and Zhang J: MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis. Oncol Lett. 6:1427–1434. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Kim KS, Kim JS, Lee MR, Jeong HS and Kim J: A study of microRNAs in silico and in vivo: Emerging regulators of embryonic stem cells. FEBS J. 276:2140–2149. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Nakata K, Ohuchida K, Mizumoto K, Aishima S, Oda Y, Nagai E and Tanaka M: MicroRNA-373 is down-regulated in pancreatic cancer and inhibits cancer cell invasion. Ann Surg Oncol. 21 Suppl 4:S564–S574. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Peng Z, Wang CX, Fang EH, Wang GB and Tong Q: Role of epithelial-mesenchymal transition in gastric cancer initiation and progression. World J Gastroenterol. 20:5403–5410. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Tanaka T, Arai M, Wu S, Kanda T, Miyauchi H, Imazeki F, Matsubara H and Yokosuka O: Epigenetic silencing of microRNA-373 plays an important role in regulating cell proliferation in colon cancer. Oncol Rep. 26:1329–1335. 2011.PubMed/NCBI

27 

Cao Y, DePinho RA, Ernst M and Vousden K: Cancer research: Past, present and future. Nat Rev Cancer. 11:749–754. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Han C, Shen JK, Hornicek FJ, Kan Q and Duan Z: Regulation of microRNA-1 (miR-1)expression in human cancer. Biochim Biophys Acta. 1860:227–232. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Matsuda A, Yan IK, Foye C, Parasramka M and Patel T: MicroRNAs as paracrine signaling mediators in cancers and metablic diseases. Best Pract Res Clin Endocrinol Metab. 30:577–590. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Huang Q, Gumireddy K, Schrier M, Le Sage C, Nagel R, Nair S, Egan DA, Li A, Huang G, Klein-Szanto AJ, et al: The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis. Nat Cell Bio. 10:202–210. 2008. View Article : Google Scholar

31 

Arzumanyan A, Friedman T, Kotei E, Ng IO, Lian Z and Feitelson MA: Epigeneticrepression of E-cadherin expression by hepatitis B virus × antigen in liver cancer. Oncogene. 31:563–572. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Stacy AJ, Craig MP, Sakaram S and Kadakia M: ΔNp63α and microRNAs: Leveraging the epithelial-mesenchymal transition. Oncotarget. 8:2114–2129. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Liu P and Wilson MJ: miR-520c and miR-373 upregulate MMP9 expression by targeting mTOR and SIRT1 and activate the Ras/Raf/MEK/Erk signaling pathway and NF-κB factor in human fibrosarcoma cells. J Cell Physiol. 227:867–876. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Wu W, He X, Kong J and Ye B: Mir-373 affects human lung cancer cells' growth and its E-cadherin expression. Oncol Res. 20:163–170. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi Y, Shi H, Zhang B, Yan Y, Han X, Jiang W, Qian H and Xu W: miR-373 suppresses gastric cancer metastasis by downregulating vimentin. Mol Med Rep 17: 4027-4034, 2018.
APA
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W. ... Xu, W. (2018). miR-373 suppresses gastric cancer metastasis by downregulating vimentin. Molecular Medicine Reports, 17, 4027-4034. https://doi.org/10.3892/mmr.2017.8291
MLA
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W., Qian, H., Xu, W."miR-373 suppresses gastric cancer metastasis by downregulating vimentin". Molecular Medicine Reports 17.3 (2018): 4027-4034.
Chicago
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W., Qian, H., Xu, W."miR-373 suppresses gastric cancer metastasis by downregulating vimentin". Molecular Medicine Reports 17, no. 3 (2018): 4027-4034. https://doi.org/10.3892/mmr.2017.8291
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Shi H, Zhang B, Yan Y, Han X, Jiang W, Qian H and Xu W: miR-373 suppresses gastric cancer metastasis by downregulating vimentin. Mol Med Rep 17: 4027-4034, 2018.
APA
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W. ... Xu, W. (2018). miR-373 suppresses gastric cancer metastasis by downregulating vimentin. Molecular Medicine Reports, 17, 4027-4034. https://doi.org/10.3892/mmr.2017.8291
MLA
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W., Qian, H., Xu, W."miR-373 suppresses gastric cancer metastasis by downregulating vimentin". Molecular Medicine Reports 17.3 (2018): 4027-4034.
Chicago
Shi, Y., Shi, H., Zhang, B., Yan, Y., Han, X., Jiang, W., Qian, H., Xu, W."miR-373 suppresses gastric cancer metastasis by downregulating vimentin". Molecular Medicine Reports 17, no. 3 (2018): 4027-4034. https://doi.org/10.3892/mmr.2017.8291
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