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Article

Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits

  • Authors:
    • Xiaoling Fu
    • Ruiyun Qiu
    • Chunfang Tang
    • Xiaomei Wang
    • Xigao Cheng
    • Ming Yin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, College of Information Engineering, Nanchang University, Nanchang, Jiangxi 330031, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Nanchang, Jiangxi 341099, P.R. China
  • Pages: 3821-3828
    |
    Published online on: December 18, 2017
       https://doi.org/10.3892/mmr.2017.8304
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Abstract

Effective therapeutic methods for osteoarthritis (OA) are lacking. γ‑glutamyl carboxylase (GGCX) is a key enzyme that regulates carboxylation of cartilage matrix Gla protein (MGP). Whether GGCX overexpression protects against OA remains unknown. The aim of the present study was to explore the effects of GGCX overexpression on anterior cruciate ligament transection (ACLT)‑induced OA and its mechanisms in Japanese white rabbits. ACLT surgery was used to establish an OA model in rabbits. A total of 48 rabbits were randomly divided into 4 groups: Sham, OA model + GGCX overexpression plasmid, OA model + saline and OA model + empty vector. The expression of uncarboxylated MGP (ucMGP), carboxylated MGP (cMGP), matrix metalloproteinase (MMP)‑13, collagen type X, collagen type II, tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were detected by ELISA, immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blotting. Morphological changes to tibial cartilage were assessed by Giemsa and safranin O‑fast green staining, respectively. Compared with the Sham control, GGCX expression was significantly decreased in the OA Model group. GGCX expression was increased by injection of a lentivirus‑carried overexpression plasmid that encoded GGCX. GGCX overexpression ameliorated ATLC‑induced damage in articular cartilage. OA Model rabbits exhibited significantly decreased expression levels of cMGP and collagen type II, and increased expression of ucMGP, collagen type X, MMP‑13, IL‑1β and TNF‑α. Notably, these expression levels were reversed by GGCX overexpression in OA Model rabbits. Results from the present study indicated that GGCX expression was decreased in OA Model rabbits, whereas overexpression of GGCX was able to promote carboxylation of MGP, reduce inflammation, decrease MMP‑13 expression and regulate collagen expression. The results also indicated that GGCX may serve as a therapeutic target for OA.
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View References

1 

Loeser RF, Goldring SR, Scanzello CR and Goldring MB: Osteoarthritis: A disease of the joint as an organ. Arthritis Rheum. 64:1697–1707. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Crema MD, Roemer FW, Felson DT, Englund M, Wang K, Jarraya M, Nevitt MC, Marra MD, Torner JC, Lewis CE and Guermazi A: Factors associated with meniscal extrusion in knees with or at risk for osteoarthritis: The multicenter osteoarthritis study. Radiology. 264:494–503. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Michael JW, Schlüter-Brust KU and Eysel P: The epidemiology, etiology, diagnosis, and treatment of osteoarthritis of the knee. Dtsch Arztebl Int. 107:152–162. 2010.PubMed/NCBI

4 

Zhang Y and Jordan JM: Epidemiology of osteoarthritis. Clin Geriatr Med. 26:355–369. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Geusens PP and van den Bergh JP: Osteoporosis and osteoarthritis: Shared mechanisms and epidemiology. Curr Opin Rheumatol. 28:97–103. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Shea MK, O'Donnell CJ, Vermeer C, Magdeleyns EJ, Crosier MD, Gundberg CM, Ordovas JM, Kritchevsky SB and Booth SL: Circulating uncarboxylated matrix gla protein is associated with vitamin K nutritional status, but not coronary artery calcium, in older adults. J Nutr. 141:1529–1534. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Silaghi CN, Fodor D, Cristea V and Craciun AM: Synovial and serum levels of uncarboxylated matrix Gla-protein (ucMGP) in patients with arthritis. Clin Chem Lab Med. 50:125–128. 2012. View Article : Google Scholar

8 

Xi XF, Li XZ, Liu F, Fu NN, Ren Y, Yang XG and Zhang Y: Effects of short thrust needing plus Electroacupuncture intervention on cartilage tissue in rabbits with knee osteoarthritis. Zhen Ci Yan Jiu. 41:124–130. 2016.(In Chinese). PubMed/NCBI

9 

Shi Y, Chen W and Yan S: Study on effect of GGCX in knee osteoarthritis pathogenesis. Int J Clin Exp Med. 9:13657–13663. 2016.

10 

Sokolove J and Lepus CM: Role of inflammation in the pathogenesis of osteoarthritis: Latest findings and interpretations. Ther Adv Musculoskelet Dis. 5:77–94. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Bello AE and Oesser S: Collagen hydrolysate for the treatment of osteoarthritis and other joint disorders: A review of the literature. Curr Med Res Opin. 22:2221–2232. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Boulocher C, Duclos ME, Arnault F, Roualdes O, Fau D, Hartmann DJ, Roger T, Vignon E and Viguier E: Knee joint ultrasonography of the ACLT rabbit experimental model of osteoarthritis: Relevance and effectiveness in detecting meniscal lesions. Osteoarthritis Cartilage. 16:470–479. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Kurz B, Domm C, Jin M, Sellckau R and Schünke M: Tissue engineering of articular cartilage under the influence of collagen I/III membranes and low oxygen tension. Tissue Eng. 10:1277–1286. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Arden N and Nevitt MC: Osteoarthritis: Epidemiology. Best Pract Res Clin Rheumatol. 20:3–25. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Phillips S, Silvia Li C, Phillips M, Bischoff M, Ali P, Chahal J, Snider M and Bhandari M: Treatment of osteoarthritis of the Knee with bracing: A scoping review. Orthop Rev (Pavia). 8:62562016. View Article : Google Scholar : PubMed/NCBI

17 

Anandacoomarasamy A and March L: Current evidence for osteoarthritis treatments. Ther Adv Musculoskelet Dis. 2:17–28. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Viegas CS, Cavaco S, Neves PL, Ferreira A, João A, Williamson MK, Price PA, Cancela ML and Simes DC: Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Am J Pathol. 175:2288–2298. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Lorenzen JM, Martino F, Scheffner I, Bröcker V, Leitolf H, Haller H and Gwinner W: Fetuin, matrix-Gla protein and osteopontin in calcification of renal allografts. PLoS One. 7:e520392012. View Article : Google Scholar : PubMed/NCBI

20 

Berenbaum F, Griffin TM and Liu-Bryan R: Metabolic regulation of inflammation in osteoarthritis. Arthritis Rheumatol. 69:9–21. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Martin MU and Wesche H: Summary and comparison of the signaling mechanisms of the Toll/interleukin-1 receptor family. Biochim Biophys Acta. 1592:265–280. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Chambers MG, Kuffner T, Cowan SK, Cheah KS and Mason RM: Expression of collagen and aggrecan genes in normal and osteoarthritic murine knee joints. Osteoarthritis Cartilage. 10:51–61. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Eerola I, Salminen H, Lammi P, Lammi M, von der Mark K, Vuorio E and Säämänen AM: Type X collagen, a natural component of mouse articular cartilage: Association with growth, aging, and osteoarthritis. Arthritis Rheum. 41:1287–1295. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Lu P, Takai K, Weaver VM and Werb Z: Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harb Perspect Biol. 3:pii: a0050582011. View Article : Google Scholar

25 

Loeser RF, Varnum BC, Carlson CS, Goldring MB, Liu ET, Sadiev S, Kute TE and Wallin R: Human chondrocyte expression of growth-arrest-specific gene 6 and the tyrosine kinase receptor axl: Potential role in autocrine signaling in cartilage. Arthritis Rheum. 40:1455–1465. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Melaragno MG, Cavet ME, Yan C, Tai LK, Jin ZG, Haendeler J and Berk BC: Gas6 inhibits apoptosis in vascular smooth muscle: Role of Axl kinase and Akt. J Mol Cell Cardiol. 37:881–887. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Stenhoff J, Dahlbäck B and Hafizi S: Vitamin K-dependent Gas6 activates ERK kinase and stimulates growth of cardiac fibroblasts. Biochem Biophys Res Commun. 319:871–878. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fu X, Qiu R, Tang C, Wang X, Cheng X and Yin M: Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits. Mol Med Rep 17: 3821-3828, 2018.
APA
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., & Yin, M. (2018). Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits. Molecular Medicine Reports, 17, 3821-3828. https://doi.org/10.3892/mmr.2017.8304
MLA
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., Yin, M."Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits". Molecular Medicine Reports 17.3 (2018): 3821-3828.
Chicago
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., Yin, M."Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits". Molecular Medicine Reports 17, no. 3 (2018): 3821-3828. https://doi.org/10.3892/mmr.2017.8304
Copy and paste a formatted citation
x
Spandidos Publications style
Fu X, Qiu R, Tang C, Wang X, Cheng X and Yin M: Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits. Mol Med Rep 17: 3821-3828, 2018.
APA
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., & Yin, M. (2018). Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits. Molecular Medicine Reports, 17, 3821-3828. https://doi.org/10.3892/mmr.2017.8304
MLA
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., Yin, M."Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits". Molecular Medicine Reports 17.3 (2018): 3821-3828.
Chicago
Fu, X., Qiu, R., Tang, C., Wang, X., Cheng, X., Yin, M."Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits". Molecular Medicine Reports 17, no. 3 (2018): 3821-3828. https://doi.org/10.3892/mmr.2017.8304
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