Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2018 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2018 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway

  • Authors:
    • Chen Fu
    • Baoxin Chen
    • Xianglan Jin
    • Xuemei Liu
    • Fengli Wang
    • Rongjuan Guo
    • Zhigang Chen
    • Hong Zheng
    • Le Wang
    • Yunling Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China, Central Laboratory, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, P.R. China
  • Pages: 3877-3883
    |
    Published online on: December 19, 2017
       https://doi.org/10.3892/mmr.2017.8317
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Endothelial progenitor cell (EPC) dysfunction is associated with the formation of carotid atherosclerosis. It has been demonstrated that angiotensin II (Ang II) may impair the function of EPCs and puerarin, a natural product, possesses cardiovascular protective effects against oxidative stress and inflammation. Therefore, the present study aimed to investigate the beneficial effects of puerarin in Ang II‑induced EPC injury, and to elucidate the underlying mechanisms. Treatment with Ang II suppressed EPC proliferation and migration, increased the expression of the senescence marker β‑galactosidase, and the adhesion molecules intracellular adhesion molecule‑1 and vascular cell adhesion molecule‑1. However, the above effects were markedly alleviated by treatment with puerarin in a dose‑dependent manner (1, 10 and 100 µM). In addition, Ang II significantly increased reactive oxygen species production and the levels of the inflammatory cytokine tumor necrosis factor‑α and interleukin‑6. Notably, these effects were reversed by puerarin. However, it was identified that the impaired EPC functions were due to inhibition of the phosphorylation of extracellular signal‑regulated kinase 1 and 2 (ERK1/2) and the degradation of nuclear factor erythroid 2 like 2 (Nrf2), and treatment with puerarin activated the ERK1/2‑Nrf2 signaling pathway. The results of the present study indicated that puerarin protected Ang II‑induced EPC dysfunction via activation of the ERK1/2‑Nrf2 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Yin Y, Liu H, Wang F, Li L, Deng M, Huang L and Zhao X: Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats. Stem Cell Res Ther. 6:372015. View Article : Google Scholar : PubMed/NCBI

2 

Zhu S, Malhotra A, Zhang L, Deng S, Zhang T, Freedman NJ, Storms R, Peppel K, Goldschmidt-Clermont PJ and Dong C: Human umbilical cord blood endothelial progenitor cells decrease vein graft neointimal hyperplasia in SCID mice. Atherosclerosis. 212:63–69. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Ke X, Shu XR, Wu F, Hu QS, Deng BQ, Wang JF and Nie RQ: Overexpression of the β2AR gene improves function and re-endothelialization capacity of EPCs after arterial injury in nude mice. Stem Cell Res Ther. 7:732016. View Article : Google Scholar : PubMed/NCBI

4 

Briasoulis A, Tousoulis D, Antoniades C, Papageorgiou N and Stefanadis C: The role of endothelial progenitor cells in vascular repair after arterial injury and atherosclerotic plaque development. Cardiovasc Ther. 29:125–139. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Fadini GP, Coracina A, Baesso I, Agostini C, Tiengo A, Avogaro A and de Kreutzenberg SV: Peripheral blood CD34+KDR+ endothelial progenitor cells are determinants of subclinical atherosclerosis in a middle-aged general population. Stroke. 37:2277–2282. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Lau KK, Chan YH, Yiu KH, Li SW, Tam S, Lau CP, Kwong YL and Tse HF: Burden of carotid atherosclerosis in patients with stroke: Relationships with circulating endothelial progenitor cells and hypertension. J Human Hypertens. 21:445–451. 2007. View Article : Google Scholar

7 

Lin H, Pan S, Meng L, Zhou C, Jiang C, Ji Z, Chi J and Guo H: MicroRNA-384-mediated Herpud1 upregulation promotes angiotensin II-induced endothelial cell apoptosis. Biochem Biophys Res Commun. 488:453–460. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Calò LA, Facco M, Davis PA, Pagnin E, Maso LD, Puato M, Caielli P, Agostini C and Pessina AC: Endothelial progenitor cells relationships with clinical and biochemical factors in a human model of blunted angiotensin II signaling. Hypertens Res. 34:1017–1022. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Parzonko A, Czerwińska ME, Kiss AK and Naruszewicz M: Oleuropein and oleacein may restore biological functions of endothelial progenitor cells impaired by angiotensin II via activation of Nrf2/heme oxygenase-1 pathway. Phytomedicine. 20:1088–1094. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wong KH, Li GQ, Li KM, Razmovski-Naumovski V and Chan K: Kudzu root: Traditional uses and potential medicinal benefits in diabetes and cardiovascular diseases. J Ethnopharmacol. 134:584–607. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Prasain JK, Peng N, Rajbhandari R and Wyss JM: The Chinese Pueraria root extract (Pueraria lobata) ameliorates impaired glucose and lipid metabolism in obese mice. Phytomedicine. 20:17–23. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Teng Y, Cui H, Yang M, Song H, Zhang Q, Su Y and Zheng J: Protective effect of puerarin on diabetic retinopathy in rats. Mol Biol Rep. 36:1129–1133. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Meng XH, Ni C, Zhu L, Shen YL, Wang LL and Chen YY: Puerarin protects against high glucose-induced acute vascular dysfunction: Role of heme oxygenase-1 in rat thoracic aorta. Vascul Pharmacol. 50:110–115. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Hu W, Zhang Q, Yang X, Wang Y and Sun L: Puerarin inhibits adhesion molecule expression in tnf-alpha-stimulated human endothelial cells via modulation of the nuclear factor kappaB pathway. Pharmacology. 85:27–35. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Chen G, Pan SQ, Shen C, Pan SF, Zhang XM and He QY: Puerarin inhibits angiotensin II-induced cardiac hypertrophy via the redox-sensitive ERK1/2, p38 and NF-kappaB pathways. Acta Pharmacol Sin. 35:463–475. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Hill JM, Zalos G, Halcox JP, Schenke WH, Waclawiw MA, Quyyumi AA and Finkel T: Circulating endothelial progenitor cells, vascular function, and cardiovascular risk. N Engl J Med. 348:593–600. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Parzonko A, Oswit A, Bazylko A and Naruszewicz M: Anthocyans-rich Aronia melanocarpa extract possesses ability to protect endothelial progenitor cells against angiotensin II induced dysfunction. Phytomedicine. 22:1238–1246. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Li X, Lin Y, Zhou H, Li Y, Wang A, Wang H and Zhou MS: Puerarin protects against endothelial dysfunction and end-organ damage in Ang II-induced hypertension. Clin Exp Hypertens. 39:58–64. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Gong X, Shao L, Fu YM and Zou Y: Effects of olmesartan on endothelial progenitor cell mobilization and function in carotid atherosclerosis. Med Sci Monit. 21:1189–1193. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Yoder MC: Endothelial progenitor cell: A blood cell by many other names may serve similar functions. J Mol Med (Berl). 91:285–295. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Bian F, Cui J, Zheng T and Jin S: Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells. Int J Mol Med. 2017. View Article : Google Scholar

22 

Li W, Li Z, Chen Y, Li S, Lv Y, Zhou W, Liao M, Zhu F, Zhou Z, Cheng X, et al: Autoantibodies targeting AT1 receptor from patients with acute coronary syndrome upregulate proinflammatory cytokines expression in endothelial cells involving NF-κB pathway. J Immunol Res. 2014:3426932014. View Article : Google Scholar : PubMed/NCBI

23 

Han T, Liu M and Yang S: DJ-1 alleviates angiotensin II-induced endothelial progenitor cell damage by activating the PPARγ/HO-1 pathway. J Cell Biochem. 119:392–400. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Xiong FL, Sun XH, Gan L, Yang XL and Xu HB: Puerarin protects rat pancreatic islets from damage by hydrogen peroxide. Eur J Pharmacol. 529:1–7. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Yan LP, Zhuang YL, Chan SW, Chen SL and Shi GG: Analysis of the mechanisms underlying the endothelium-dependent antivasoconstriction of puerarin in rat aorta. Naunyn Schmiedebergs Arch Pharmacol. 379:587–597. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Hsu FL, Liu IM, Kuo DH, Chen WC, Su HC and Cheng JT: Antihyperglycemic effect of puerarin in streptozotocin-induced diabetic rats. J Nat Prod. 66:788–792. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Lu XL, Liu JX, Wu Q, Long SM, Zheng MY, Yao XL, Ren H, Wang YG, Su WW and Fai Cheung RT: Protective effects of puerarin against Aß40-induced vascular dysfunction in zebrafish and human endothelial cells. Eur J Pharmacol. 732:76–85. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Ji L, Du Q, Li Y and Hu W: Puerarin inhibits the inflammatory response in atherosclerosis via modulation of the NF-κB pathway in a rabbit model. Pharmacol Rep. 68:1054–1059. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Chang CC, Chu CF, Wang CN, Wu HT, Bi KW, Pang JH and Huang ST: The anti-atherosclerotic effect of tanshinone IIA is associated with the inhibition of TNF- α-induced VCAM-1, ICAM-1 and CX3CL1 expression. Phytomedicine. 21:207–216. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Spigoni V, Picconi A, Cito M, Ridolfi V, Bonomini S, Casali C, Zavaroni I, Gnudi L, Metra M and Dei Cas A: Pioglitazone improves in vitro viability and function of endothelial progenitor cells from individuals with impaired glucose tolerance. Plos One. 7:e482832012. View Article : Google Scholar : PubMed/NCBI

31 

Hwang YP, Kim HG, Hien TT, Jeong MH, Jeong TC and Jeong HG: Puerarin activates endothelial nitric oxide synthase through estrogen receptor-dependent PI3-kinase and calcium-dependent AMP-activated protein kinase. Toxicol Appl Pharmacol. 257:48–58. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Kang OH, Kim SB, Mun SH, Seo YS, Hwang HC, Lee YM, Lee HS, Kang DG and Kwon DY: Puerarin ameliorates hepatic steatosis by activating the PPARalpha and AMPK signaling pathways in hepatocytes. Int J Mol Med. 35:803–809. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Fu C, Chen B, Jin X, Liu X, Wang F, Guo R, Chen Z, Zheng H, Wang L, Zhang Y, Zhang Y, et al: Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway. Mol Med Rep 17: 3877-3883, 2018.
APA
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R. ... Zhang, Y. (2018). Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway. Molecular Medicine Reports, 17, 3877-3883. https://doi.org/10.3892/mmr.2017.8317
MLA
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R., Chen, Z., Zheng, H., Wang, L., Zhang, Y."Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway". Molecular Medicine Reports 17.3 (2018): 3877-3883.
Chicago
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R., Chen, Z., Zheng, H., Wang, L., Zhang, Y."Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway". Molecular Medicine Reports 17, no. 3 (2018): 3877-3883. https://doi.org/10.3892/mmr.2017.8317
Copy and paste a formatted citation
x
Spandidos Publications style
Fu C, Chen B, Jin X, Liu X, Wang F, Guo R, Chen Z, Zheng H, Wang L, Zhang Y, Zhang Y, et al: Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway. Mol Med Rep 17: 3877-3883, 2018.
APA
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R. ... Zhang, Y. (2018). Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway. Molecular Medicine Reports, 17, 3877-3883. https://doi.org/10.3892/mmr.2017.8317
MLA
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R., Chen, Z., Zheng, H., Wang, L., Zhang, Y."Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway". Molecular Medicine Reports 17.3 (2018): 3877-3883.
Chicago
Fu, C., Chen, B., Jin, X., Liu, X., Wang, F., Guo, R., Chen, Z., Zheng, H., Wang, L., Zhang, Y."Puerarin protects endothelial progenitor cells from damage of angiotensin II via activation of ERK1/2‑Nrf2 signaling pathway". Molecular Medicine Reports 17, no. 3 (2018): 3877-3883. https://doi.org/10.3892/mmr.2017.8317
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team