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Article

BAMBI promotes macrophage proliferation and differentiation in gliomas

  • Authors:
    • Dan Wang
    • Xiaolai Chen
    • Ran Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan 410005, P.R. China, Department of Immunology, Hunan Normal University School of Medicine, Changsha, Hunan 410031, P.R. China
  • Pages: 3960-3966
    |
    Published online on: December 20, 2017
       https://doi.org/10.3892/mmr.2017.8320
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Abstract

The present study investigated the capacity of Bone morphogenic protein and activin membrane‑bound inhibitor homolog (BAMBI) to regulate the migration and differentiation of macrophages in gliomas. Using a migration assay, it was determined that BAMBI stimulated monocytes migration in a dose‑dependent effect. When induced by phorbol myristate acetate (PMA) the monocytes differentiated into macrophages, and BAMBI also increased the migration of PMA‑induced macrophages compared with control cells. The expression of CD68 and BAMBI protein and mRNA in glioma and normal specimens were detected using immunohistochemistry and reverse transcription‑quantitative polymerase chain reaction, respectively. The localization of BAMBI was primarily in macrophages, as demonstrated by staining for the macrophage marker CD68, and the mRNA expression of CD68 and BAMBI were higher in gliomas compared to normal tissues. In addition, the mRNA expression of CD68 and BAMBI were positively correlated (R2=0.64). After treatment with 50 nM PMA and 10 nM BAMBI for 48 h, RAW 264.7 macrophages were exhibited dendrite‑like morphology, indicating that the co‑treatment promoted the differentiation of monocytes to macrophages. The expression of specific markers of M1 [inducible nitric oxide synthase (iNOS) and interleukin (IL)-12] and M2 (IL-10 and arginase 1) type macrophages was determined following 10 nM BAMBI treatment. BAMBI promoted the expression of M1 markers, whereas the M2 markers were not affected, which indicated that BAMBI induced differentiation of M1 type macrophages. These results indicate that BAMBI may be involved in macrophage differentiation in gliomas.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Chen X and Zhang R: BAMBI promotes macrophage proliferation and differentiation in gliomas. Mol Med Rep 17: 3960-3966, 2018.
APA
Wang, D., Chen, X., & Zhang, R. (2018). BAMBI promotes macrophage proliferation and differentiation in gliomas. Molecular Medicine Reports, 17, 3960-3966. https://doi.org/10.3892/mmr.2017.8320
MLA
Wang, D., Chen, X., Zhang, R."BAMBI promotes macrophage proliferation and differentiation in gliomas". Molecular Medicine Reports 17.3 (2018): 3960-3966.
Chicago
Wang, D., Chen, X., Zhang, R."BAMBI promotes macrophage proliferation and differentiation in gliomas". Molecular Medicine Reports 17, no. 3 (2018): 3960-3966. https://doi.org/10.3892/mmr.2017.8320
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Chen X and Zhang R: BAMBI promotes macrophage proliferation and differentiation in gliomas. Mol Med Rep 17: 3960-3966, 2018.
APA
Wang, D., Chen, X., & Zhang, R. (2018). BAMBI promotes macrophage proliferation and differentiation in gliomas. Molecular Medicine Reports, 17, 3960-3966. https://doi.org/10.3892/mmr.2017.8320
MLA
Wang, D., Chen, X., Zhang, R."BAMBI promotes macrophage proliferation and differentiation in gliomas". Molecular Medicine Reports 17.3 (2018): 3960-3966.
Chicago
Wang, D., Chen, X., Zhang, R."BAMBI promotes macrophage proliferation and differentiation in gliomas". Molecular Medicine Reports 17, no. 3 (2018): 3960-3966. https://doi.org/10.3892/mmr.2017.8320
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