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Article Open Access

The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats

  • Authors:
    • Yong Zhang
    • Li‑Tang Fu
    • Fang Tang
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Dingzhou Radi‑Glory Bio‑Chem Co., Ltd., Baoding, Hebei 073000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3455-3464
    |
    Published online on: December 20, 2017
       https://doi.org/10.3892/mmr.2017.8321
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Abstract

The present study aimed to investigate the protective effects of magnolol on acute 2,4,6-trinitrobenzene sulfonic acid (TNBS)‑induced colitis, and its underlying mechanisms. Experimental colitis was induced by intracolonic administration of TNBS/ethanol into rats. The model rats were randomly assigned into groups: TNBS, magnolol (high, medium and low doses), and salazosulfapyridine (positive control). All intervention regimens were administered by oral gavage, once a day for 7 consecutive days, 24 h after colitis induction. Histological and biochemical changes in colonic inflammation were evaluated by hematoxylin and eosin and immunohistochemistry, respectively. Rats treated with all doses of magnolol exhibited decreased colonic myeloperoxidase activity (P<0.05 vs. TNBS), reduced serum levels of proinflammatory cytokines [including interleukin (IL)‑6 and IL‑17], and downregulated Toll‑like receptor-4 (TLR‑4) mRNA expression. Histological analysis revealed that medium and high doses of magnolol conferred an anti‑inflammatory effect, which was indicated by a decrease in disease activity index, an increase in thymus index, and downregulation of nuclear factor (NF)‑κB p65 mRNA and TLR‑4 protein expression. However, only high‑dose magnolol significantly ameliorated the elevated colon weight/length ratio. The results of the present study indicate that magnolol exerts protective effects against acute TNBS‑induced colitis in rats, and the TLR‑4/NF‑κB signaling pathway‑mediated inhibitory effect on inflammatory cascades may contribute to the protective activity of magnolol.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Fu LT and Tang F: The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats. Mol Med Rep 17: 3455-3464, 2018.
APA
Zhang, Y., Fu, L., & Tang, F. (2018). The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats. Molecular Medicine Reports, 17, 3455-3464. https://doi.org/10.3892/mmr.2017.8321
MLA
Zhang, Y., Fu, L., Tang, F."The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats". Molecular Medicine Reports 17.3 (2018): 3455-3464.
Chicago
Zhang, Y., Fu, L., Tang, F."The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats". Molecular Medicine Reports 17, no. 3 (2018): 3455-3464. https://doi.org/10.3892/mmr.2017.8321
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Fu LT and Tang F: The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats. Mol Med Rep 17: 3455-3464, 2018.
APA
Zhang, Y., Fu, L., & Tang, F. (2018). The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats. Molecular Medicine Reports, 17, 3455-3464. https://doi.org/10.3892/mmr.2017.8321
MLA
Zhang, Y., Fu, L., Tang, F."The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats". Molecular Medicine Reports 17.3 (2018): 3455-3464.
Chicago
Zhang, Y., Fu, L., Tang, F."The protective effects of magnolol on acute trinitrobenzene sulfonic acid‑induced colitis in rats". Molecular Medicine Reports 17, no. 3 (2018): 3455-3464. https://doi.org/10.3892/mmr.2017.8321
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