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Article

Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure

  • Authors:
    • Xia Li
    • Xuan Tang
    • Jinping Lu
    • Sheng Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Laboratory Medicine, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei 434020, P.R. China
  • Pages: 4106-4112
    |
    Published online on: December 20, 2017
       https://doi.org/10.3892/mmr.2017.8323
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Abstract

Galectin-3 is an important mediator of cardiac fibrosis and heart failure. Cell viability of cardiomyocytes was measured using a CCK‑8 assay; flow cytometry was employed for the detection of the cell cycle and cardiomyocytes apoptosis. Reverse transcription‑quantitative polymerase chain reaction and western blotting was performed to examine the expression of associated genes and proteins. The present study demonstrated that overexpression of galectin‑3 significantly decreased the viability of cardiomyocytes in a time‑dependent manner, with simultaneous arrest of the cell cycle and induction of apoptosis. The expression levels of proliferating cell nuclear antigen (PCNA) and B‑cell lymphoma 2 (Bcl‑2) were decreased and Bcl‑2‑associated X protein (Bax) was increased in cardiomyocytes with galectin‑3 overexpression. However, inhibition of galectin‑3 by intravenous tail vein injection of a galectin‑3‑targeting short hairpin RNA‑expressing vector during hypertension‑induced heart failure in Dahl hypertensive rats increased rat survival and body weight. Inhibition of galectin‑3 also increased the expression of PCNA and Bcl‑2 and reduced the expression of Bax in the cardiac tissue of hypertensive rats. These results demonstrate the therapeutic potential of targetinggalectin‑3 for the treatment of cardiac disease.
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1 

Montgomery RL, Hullinger TG, Semus HM, Dickinson BA, Seto AG, Lynch JM, Stack C, Latimer PA, Olson EN and van Rooij E: Therapeutic inhibition of miR-208a improves cardiac function and survival during heart failure. Circulation. 124:1537–1547. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, de Ferranti S, Després JP, Fullerton HJ, Howard VJ, et al: Heart disease and stroke statistics-2015 update: A report from the American heart association. Circulation. 131:e29–e322. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Guha K and McDonagh T: Heart failure epidemiology: European perspective. Curr Cardiol Rev. 9:123–127. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Hu SS, Kong LZ, Gao RL, Zhu ML, Wang W, Wang YJ, Wu ZS, Chen WW and Liu MB; Editorial Board, : Outline of the report on cardiovascular disease in China, 2010. Biomed Environ Sci. 25:251–256. 2012.PubMed/NCBI

5 

Bui AL, Horwich TB and Fonarow GC: Epidemiology and risk profile of heart failure. Nat Rev Cardiol. 8:30–41. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Spinelli L, Stabile E, Giugliano G, Morisco C, Giudice CA, Imbriaco M, Santoro M, Esposito G and Trimarco B: Intramyocardial dissecting hematoma in anterior wall ST elevation myocardial infarction: Impact on left ventricular remodeling and prognosis. Int J Cardiovasc Imaging. Aug 1–2017.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

7 

Sharma UC, Pokharel S, van Brakel TJ, van Berlo JH, Cleutjens JP, Schroen B, André S, Crijns HJ, Gabius HJ, Maessen J and Pinto YM: Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110:3121–3128. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Liu YH, D'Ambrosio M, Liao TD, Peng H, Rhaleb NE, Sharma U, André S, Gabius HJ and Carretero OA: N-acetyl-seryl-aspartyl-lysyl-proline prevents cardiac remodeling and dysfunction induced by galectin-3, a mammalian adhesion/growth-regulatory lectin. Am J Physiol Heart Circ Physiol. 296:H404–H412. 2009. View Article : Google Scholar : PubMed/NCBI

9 

de Boer RA, Yu L and van Veldhuisen DJ: Galectin-3 in cardiac remodeling and heart failure. Curr Heart Fail Rep. 7:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Henderson NC, Mackinnon AC, Farnworth SL, Poirier F, Russo FP, Iredale JP, Haslett C, Simpson KJ and Sethi T: Galectin-3 regulates myofibroblast activation and hepatic fibrosis. Proc Natl Acad Sci USA. 103:pp. 5060–5065. 2006; View Article : Google Scholar : PubMed/NCBI

11 

Henderson NC, Mackinnon AC, Farnworth SL, Kipari T, Haslett C, Iredale JP, Liu FT, Hughes J and Sethi T: Galectin-3 expression and secretion links macrophages to the promotion of renal fibrosis. Am J Pathol. 172:288–298. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Van den Borne SW, Diez J, Blankesteijn WM, Verjans J, Hofstra L and Narula J: Myocardial remodeling after infarction: The role of myofibroblasts. Nat Rev Cardiol. 7:30–37. 2010. View Article : Google Scholar : PubMed/NCBI

13 

de Boer RA, Voors AA, Muntendam P, van Gilst WH and van Veldhuisen DJ: Galectin-3: A novel mediator of heart failure development and progression. Eur J Heart Fail. 11:811–817. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Anand IS, Rector TS, Kuskowski M, Adourian A, Muntendam P and Cohn JN: Baseline and serial measurements of galectin-3 in patients with heart failure: Relationship to prognosis and effect of treatment with valsartan in the Val-HeFT. Eur J Heart Fail. 15:511–518. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wei F, Wang TZ, Zhang J, Yuan ZY, Tian HY, Ni YJ, Zhuo XZ, Han K, Liu Y, Lu Q, et al: Mesenchymal stem cells neither fully acquire the electrophysiological properties of mature cardiomyocytes nor promote ventricular arrhythmias in infarcted rats. Basic Res Cardiol. 107:2742012. View Article : Google Scholar : PubMed/NCBI

16 

Rodenbaugh DW, Wang W, Davis J, Edwards T, Potter JD and Metzger JM: Parvalbumin isoforms differentially accelerate cardiac myocyte relaxation kinetics in an animal model of diastolic dysfunction. Am J Physiol Heart Circ Physiol. 293:H1705–H1713. 2007. View Article : Google Scholar : PubMed/NCBI

17 

National Institutes of Health, . Guide for the Care and Use of Laboratory AnimalsNIH publication no. 85_/23. National Institutes of Health; Rockville, MD: 1996

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Wang XH, Zhuo XZ, Ni YJ, Gong M, Wang TZ, Lu Q and Ma AQ: Improvement of cardiac function and reversal of gap junction remodeling by neuregulin-1β in volume-overloaded rats with heart failure. J Geriatr Cardiol. 9:172–179. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Niwa M, Miwa Y, Kuzuya T, Iwasaki K, Haneda M, Ueki T, Katayama A, Hiramitsu T, Goto N, Nagasaka T, et al: Stimulation index for PCNA mRNA in peripheral blood as immune function monitoring after renal transplantation. Transplantation. 87:1411–1414. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Hetz C: BCL-2 protein family. Essential regulators of cell death. Preface. Adv Exp Med Biol. 687:vii–viii. 2010.PubMed/NCBI

22 

Hrynchyshyn N, Jourdain P, Desnos M, Diebold B and Funck F: Galectin-3: A new biomarker for the diagnosis, analysis and prognosis of acute and chronic heart failure. Arch Cardiovasc Dis. 106:541–546. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Gaggin HK and Januzzi JL Jr: Biomarkers and diagnostics in heart failure. Biochim Biophys Acta. 1832:2442–2450. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Ho JE, Liu C, Lyass A, Courchesne P, Pencina MJ, Vasan RS, Larson MG and Levy D: Galectin-3, a marker of cardiac fibrosis, predicts incident heart failure in the community. J Am Coll Cardiol. 60:1249–1256. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Yoshii T, Fukumori T, Honjo Y, Inohara H, Kim HR and Raz A: Galectin-3 phosphorylation is required for its anti-apoptotic function and cell cycle arrest. J Biol Chem. 277:6852–6857. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Lin HM, Pestell RG, Raz A and Kim HR: Galectin-3 enhances cyclin D(1) promoter activity through SP1 and a cAMP-responsive element in human breast epithelial cells. Oncogene. 21:8001–8010. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Yu L, Ruifrok WP, Meissner M, Bos EM, van Goor H, Sanjabi B, van der Harst P, Pitt B, Goldstein IJ, Koerts JA, et al: Genetic and pharmacological inhibition of galectin-3 prevents cardiac remodeling by interfering with myocardial fibrogenesis. Circ Heart Fail. 6:107–117. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Tang X, Lu J and Yuan S: Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure. Mol Med Rep 17: 4106-4112, 2018.
APA
Li, X., Tang, X., Lu, J., & Yuan, S. (2018). Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure. Molecular Medicine Reports, 17, 4106-4112. https://doi.org/10.3892/mmr.2017.8323
MLA
Li, X., Tang, X., Lu, J., Yuan, S."Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure". Molecular Medicine Reports 17.3 (2018): 4106-4112.
Chicago
Li, X., Tang, X., Lu, J., Yuan, S."Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure". Molecular Medicine Reports 17, no. 3 (2018): 4106-4112. https://doi.org/10.3892/mmr.2017.8323
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Tang X, Lu J and Yuan S: Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure. Mol Med Rep 17: 4106-4112, 2018.
APA
Li, X., Tang, X., Lu, J., & Yuan, S. (2018). Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure. Molecular Medicine Reports, 17, 4106-4112. https://doi.org/10.3892/mmr.2017.8323
MLA
Li, X., Tang, X., Lu, J., Yuan, S."Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure". Molecular Medicine Reports 17.3 (2018): 4106-4112.
Chicago
Li, X., Tang, X., Lu, J., Yuan, S."Therapeutic inhibition of galectin‑3 improves cardiomyocyte apoptosis and survival during heart failure". Molecular Medicine Reports 17, no. 3 (2018): 4106-4112. https://doi.org/10.3892/mmr.2017.8323
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