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Article

Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing

  • Authors:
    • Jian‑Li Dong
    • Hai‑Cao Dong
    • Liang Yang
    • Zhe‑Wen Qiu
    • Jia Liu
    • Hong Li
    • Li‑Xia Zhong
    • Xue Song
    • Peng Zhang
    • Pei‑Nan Li
    • Lian‑Jie Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Second Clinical College, Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Experimental Animal Center, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Cell Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Oncology, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, P.R. China
  • Pages: 3807-3812
    |
    Published online on: December 20, 2017
       https://doi.org/10.3892/mmr.2017.8331
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Abstract

Maggot extract (ME) accelerates rat skin wound healing, however its effect on cell maintenance in wound tissues remains unclear. B‑cell lymphoma (Bcl) 2‑associated athanogene (BAG)3 inhibits apoptosis and promotes autophagy by associating with Bcl‑2 or Beclin 1. Bcl‑2, the downstream effector of signal transducer and activator of transcription 3 signaling, is enhanced in ME‑treated wound tissues, which may reinforce the Bcl‑2 anti‑apoptotic activity and/or cooperate with Beclin 1 to regulate autophagy during wound healing. The present study investigated expression levels of BAG3, Bcl‑2, Beclin 1 and light chain (LC)3 levels in rat skin wound tissues in the presence and absence of ME treatment. The results revealed frequent TUNEL‑negative cell death in the wound tissues in the early three days following injury, irrespective to ME treatment. TUNEL‑positive cells appeared in the wound tissues following 4 days of injury and 150 µg/ml ME efficiently reduced apoptotic rate and enhanced BAG3 and Bcl‑2 expression. Elevated Beclin 1 and LC3 levels and an increased LC3 II ratio were revealed in the ME‑treated tissues during the wound healing. The results of the present study demonstrate the anti‑apoptotic effects of BAG3 and Bcl‑2 in ME‑promoted wound healing. Beclin 1/LC3 mediated autophagy may be favorable in maintaining cell survival in the damaged tissues and ME‑upregulated BAG3 may enhance its activity.
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Copy and paste a formatted citation
Spandidos Publications style
Dong JL, Dong HC, Yang L, Qiu ZW, Liu J, Li H, Zhong LX, Song X, Zhang P, Li PN, Li PN, et al: Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing. Mol Med Rep 17: 3807-3812, 2018.
APA
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H. ... Zheng, L. (2018). Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing. Molecular Medicine Reports, 17, 3807-3812. https://doi.org/10.3892/mmr.2017.8331
MLA
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H., Zhong, L., Song, X., Zhang, P., Li, P., Zheng, L."Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing". Molecular Medicine Reports 17.3 (2018): 3807-3812.
Chicago
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H., Zhong, L., Song, X., Zhang, P., Li, P., Zheng, L."Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing". Molecular Medicine Reports 17, no. 3 (2018): 3807-3812. https://doi.org/10.3892/mmr.2017.8331
Copy and paste a formatted citation
x
Spandidos Publications style
Dong JL, Dong HC, Yang L, Qiu ZW, Liu J, Li H, Zhong LX, Song X, Zhang P, Li PN, Li PN, et al: Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing. Mol Med Rep 17: 3807-3812, 2018.
APA
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H. ... Zheng, L. (2018). Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing. Molecular Medicine Reports, 17, 3807-3812. https://doi.org/10.3892/mmr.2017.8331
MLA
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H., Zhong, L., Song, X., Zhang, P., Li, P., Zheng, L."Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing". Molecular Medicine Reports 17.3 (2018): 3807-3812.
Chicago
Dong, J., Dong, H., Yang, L., Qiu, Z., Liu, J., Li, H., Zhong, L., Song, X., Zhang, P., Li, P., Zheng, L."Upregulation of BAG3 with apoptotic and autophagic activities in maggot extract‑promoted rat skin wound healing". Molecular Medicine Reports 17, no. 3 (2018): 3807-3812. https://doi.org/10.3892/mmr.2017.8331
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