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Article Open Access

Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus

  • Authors:
    • Qing Luo
    • Xue Li
    • Chuxin Xu
    • Lulu Zeng
    • Jianqing Ye
    • Yang Guo
    • Zikun Huang
    • Junming Li
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3489-3496
    |
    Published online on: December 22, 2017
       https://doi.org/10.3892/mmr.2017.8344
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Abstract

Thousands of long noncoding RNAs (lncRNAs) have been reported and represent an important subset of pervasive genes associated with a broad range of biological functions. Abnormal expression levels of lncRNAs have been demonstrated in multiple types of human disease. However, the role of lncRNAs in systemic lupus erythematosus (SLE) remains poorly understood. In the present study, the expression patterns of lncRNAs and messenger RNAs (mRNAs) were investigated in peripheral blood mononuclear cells (PBMCs) in SLE using Human lncRNA Array v3.0 (8x60 K; Arraystar, Inc., Rockville, MD, USA). The microarray results indicated that 8,868 lncRNAs (3,657 upregulated and 5,211 downregulated) and 6,876 mRNAs (2,862 upregulated and 4,014 downregulated) were highly differentially expressed in SLE samples compared with the healthy group. Gene ontology (GO) analysis of lncRNA target prediction indicated the presence of 474 matched lncRNA‑mRNA pairs for 293 differentially expressed lncRNAs (fold change, ≥3.0) and 381 differentially expressed mRNAs (fold change, ≥3.0). The most enriched pathways were ‘Transcriptional misregulation in cancer’ and ‘Valine, leucine and isoleucine degradation’. Furthermore, reverse transcription‑quantitative polymerase chain reaction data verified six abnormal lncRNAs and mRNAs in SLE. The results indicate that the lncRNA expression profile in SLE was significantly changed. In addition, a range of SLE‑associated lncRNAs were identified. Thus, the present results provide important insights regarding lncRNAs in the pathogenesis of SLE.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Q, Li X, Xu C, Zeng L, Ye J, Guo Y, Huang Z and Li J: Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Mol Med Rep 17: 3489-3496, 2018.
APA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y. ... Li, J. (2018). Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Molecular Medicine Reports, 17, 3489-3496. https://doi.org/10.3892/mmr.2017.8344
MLA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17.3 (2018): 3489-3496.
Chicago
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17, no. 3 (2018): 3489-3496. https://doi.org/10.3892/mmr.2017.8344
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Q, Li X, Xu C, Zeng L, Ye J, Guo Y, Huang Z and Li J: Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Mol Med Rep 17: 3489-3496, 2018.
APA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y. ... Li, J. (2018). Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus. Molecular Medicine Reports, 17, 3489-3496. https://doi.org/10.3892/mmr.2017.8344
MLA
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17.3 (2018): 3489-3496.
Chicago
Luo, Q., Li, X., Xu, C., Zeng, L., Ye, J., Guo, Y., Huang, Z., Li, J."Integrative analysis of long non-coding RNAs and messenger RNA expression profiles in systemic lupus erythematosus". Molecular Medicine Reports 17, no. 3 (2018): 3489-3496. https://doi.org/10.3892/mmr.2017.8344
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