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Article Open Access

Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies

  • Authors:
    • Xing‑He Xue
    • Zhen‑Hua Feng
    • Zhen‑Xing Li
    • Xiao‑Yun Pan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3751-3757
    |
    Published online on: December 27, 2017
       https://doi.org/10.3892/mmr.2017.8349
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Abstract

Dexamethasone (Dex) and other glucocorticoids are widely used to treat serious infections and immunological diseases, however they may cause steroid‑induced avascular necrosis of the femoral head (SANFH). Salidroside (Sal) has demonstrated an anti‑apoptotic effect on neurocytes by activating the phosphoinositide 3‑kinase (PI3K)/protein kinase B (Akt) signaling pathway. In the present study, primary osteoblasts were used in vitro and in rats in vivo to determine the anti-apoptotic effect of Sal on SANFH. The result of the present study demonstrated that pretreatment with Sal increased the cell survival rate while decreasing the cell apoptosis and lactate dehydrogenase release rate. Additionally, Sal also caused the reduction of TUNEL positive cells in TUNEL staining assay. Sal decreased the expression of cleaved caspase-3, cleaved caspase‑9, apoptosis regulator BAX and cytochrome C, while it increased the expression of B cell lymphoma‑2 and phosphorylated‑Akt in Dex‑induced osteoblasts. In vivo Sal protected against SANFH in rats by decreasing the percentage of empty lacunae. The present study demonstrated that Sal alleviated Dex‑induced osteoblast apoptosis by activating the PI3K/Akt signaling pathway and downregulating caspase‑3 expression in osteoblasts. Sal also protected against SANFH in a rat model of SANFH by decreasing the percentage of empty lacunae. The inhibition of the mitochondrial apoptosis pathway was also involved. Further research is required to determine the full underlying mechanisms by which Sal has an effect.
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Copy and paste a formatted citation
Spandidos Publications style
Xue XH, Feng ZH, Li ZX and Pan XY: Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies. Mol Med Rep 17: 3751-3757, 2018.
APA
Xue, X., Feng, Z., Li, Z., & Pan, X. (2018). Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies. Molecular Medicine Reports, 17, 3751-3757. https://doi.org/10.3892/mmr.2017.8349
MLA
Xue, X., Feng, Z., Li, Z., Pan, X."Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies". Molecular Medicine Reports 17.3 (2018): 3751-3757.
Chicago
Xue, X., Feng, Z., Li, Z., Pan, X."Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies". Molecular Medicine Reports 17, no. 3 (2018): 3751-3757. https://doi.org/10.3892/mmr.2017.8349
Copy and paste a formatted citation
x
Spandidos Publications style
Xue XH, Feng ZH, Li ZX and Pan XY: Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies. Mol Med Rep 17: 3751-3757, 2018.
APA
Xue, X., Feng, Z., Li, Z., & Pan, X. (2018). Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies. Molecular Medicine Reports, 17, 3751-3757. https://doi.org/10.3892/mmr.2017.8349
MLA
Xue, X., Feng, Z., Li, Z., Pan, X."Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies". Molecular Medicine Reports 17.3 (2018): 3751-3757.
Chicago
Xue, X., Feng, Z., Li, Z., Pan, X."Salidroside inhibits steroid-induced avascular necrosis of the femoral head via the PI3K/Akt signaling pathway: In vitro and in vivo studies". Molecular Medicine Reports 17, no. 3 (2018): 3751-3757. https://doi.org/10.3892/mmr.2017.8349
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