Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2018 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2018 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study

  • Authors:
    • Xiao‑Yu Li
    • Li Xu
    • Hong‑Li Li
    • Jing Du
    • Xue‑Song Liu
    • Feng‑Hua Li
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
  • Pages: 3763-3768
    |
    Published online on: December 27, 2017
       https://doi.org/10.3892/mmr.2017.8351
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to prepare and characterize an Au-poly(lactic-co-glycolic) acid (PLGA) complex, surfaced with gold nanoparticles (GNPs), and to assess its use as an ultrasound contrast agent for in vitro imaging. PLGA nanoparticles (NPs) were prepared by a modified double‑emulsion solvent evaporation method. GNPs were prepared via reduction with sodium citrate. Au‑PLGA complexes were created through the binding of polyethylene glycosylated GNPs to the surface of PLGA NPs. Various physical characteristics of PLGA NPs, GNPs and Au‑PLGA complexes, including morphology, size, dispersion, ζ potential and stability were observed and measured. The study of the in vitro imaging capability of the Au‑PLGA complex was conducted by setting multi‑parameter imaging conditions. The average sizes of PLGA NPs, GNPs and the Au‑PLGA complex were 144.5±30.28, 21.42±1.56 and 359.4±67.94 nm, respectively. Transmission electron microscopy (TEM) revealed that PLGA NPs and GNPs were spherically shaped, but GNPs had smooth surface while PLGA NPs had a capsular morphology with a wrinkled surface and large voids. The maximum absorption peak of 20 nm GNPs was 508 nm while that of the Au‑PLGA complex was 515 nm on an ultraviolet‑visible absorption spectrum curve. The results of TEM and the redshift spectrum demonstrated that GNPs successfully bound to the surface of the PLGA NPs. The complex demonstrated good stability and its size changed little over time. In vitro imaging experiments revealed that the Au‑PLGA complex had a good imaging ability. The present study demonstrated that the Au‑PLGA complex is a promising novel ultrasound contrast agent.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Khlebtsov N, Bogatyrev V, Dykman L, Khlebtsov B, Staroverov S, Shirokov A, Matora L, Khanadeev V, Pylaev T, Tsyganova N and Terentyuk G: Analytical and theranostic applications of gold nanoparticles and multifunctional nanocomposites. Theranostics. 3:167–180. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Jain S, Hirst DG and O'sullivan JM: Gold nanoparticles as novel agents for cancer therapy. Br J Radiol. 85:101–113. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Kim BY, Rutka JT and Chan WC: Nanomedicine. N Engl J Med. 363:2434–2443. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Yeh YC, Creran B and Rotello VM: Gold nanoparticles: Preparation, properties, and applications in bionanotechnology. Nanoscale. 4:1871–1880. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Song KH, Kim C, Maslov K and Wang LV: Noninvasive in vivo spectroscopic nanorod-contrast photoacoustic mapping of sentinel lymph nodes. Eur J Radiol. 70:227–231. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Néstor MM, Kei NP, Guadalupe NA, Elisa ME, Adriana GQ and David QG: Preparation and in vitro evaluation of poly(D, L-lactide-co-glycolide) air-filled nanocapsules as a contrast agent for ultrasound imaging. Ultrasonics. 51:839–845. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Kohl Y, Kaiser C, Bost W, Stracke F, Fournelle M, Wischke C, Thielecke H, Lendlein A, Kratz K and Lemor R: Preparation and biological evaluation of multifunctional PLGA-nanoparticles designed for photoacoustic imaging. Nanomedicine. 7:228–237. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Xu JS, Huang J, Qin R, Hinkle GH, Povoski SP, Martin EW and Xu RX: Synthesizing and binding dual-mode poly(lactic-co-glycolic acid) (PLGA) nanobubbles for cancer targeting and imaging. Biomaterials. 31:1716–1722. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Ke H, Wang J, Dai Z, Jin Y, Qu E, Xing Z, Guo C, Yue X and Liu J: Gold-nanoshelled microcapsules: A theranostic agent for ultrasound contrast imaging and photothermal therapy. Angew Chem Int Ed Engl. 50:3017–3021. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Xu RX, Huang J, Xu JS, Sun D, Hinkle GH, Martin EW and Povoski SP: Fabrication of indocyanine green encapsulated biodegradable microbubbles for structural and functional imaging of cancer. J Biomed Opts. 14:0340202009. View Article : Google Scholar

11 

Frens G: Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature. 241:20–22. 1973.

12 

Horisberger M and Rosset J: Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem. 25:295–305. 1977. View Article : Google Scholar : PubMed/NCBI

13 

Cózar-Bernal MJ, Holgado MA, Arias JL, Muñoz-Rubio I, Martín-Banderas L, Alvarez-Fuentes J and Fernández-Arévalo M: Insulin-loaded PLGA microparticles: Flow focusing versus double emulsion/solvent evaporation. J Microencapsul. 28:430–441. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Shive MS and Anderson JM: Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev. 28:5–24. 1997. View Article : Google Scholar : PubMed/NCBI

15 

Wang CW, Yang SP, Hu H, Du J and Li FH: Synthesis, characterization and in vitro and in vivo investigation of C3F8-filled poly(lactic-co-glycolic acid) nanoparticles as an ultrasound contrast agent. Mol Med Rep. 11:1885–1890. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Cho SH, Kim JY and Kim JD: Dynamic surface tension of stable air-filled microbubbles prepared by freeze-drying a solution of lipid/surfactant mixture. Colloids and Surfaces A Physicochemical and Engineering Aspects. 284–285, 1-457. 2006.

17 

Ma XY, Pan GM, Lu Z, Hu JS, Bei JZ, Jia JH and Wang SG: Preliminary study of oral polylactide microcapsulated insulin in vitro and in vivo. Diabetes Obes Metab. 2:243–250. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Kumari A, Yadav SK and Yadav SC: Biodegradable polymeric nanoparticles based drug delivery systems. Colloids Surf B Biointerfaces. 75:1–18. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Piñón-Segundo E, Nava-Arzaluz MG and Lechuga-Ballesteros D: Pharmaceutical polymeric nanoparticles prepared by the double emulsion-solvent evaporation technique. Recent Pat Drug Deliv Formul. 6:224–235. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Li XY, Yang ZL and Zhou HG: Studying the absorption spectrum properties of the gold nanosphere-The effect of the size on the absorption spectra of the anoparticles. J Zhangzhou Teachers Coll (Nat Sci). 17:31–34. 2004.

21 

Yuan F, Dellian M, Fukumura D, Leunig M, Berk DA, Torchilin VP and Jain RK: Vascular permeability in a human tumor xenograft: Molecular size dependence and cutoff size. Cancer Res. 55:3752–3756. 1995.PubMed/NCBI

22 

Koo OM, Rubinstein I and Onyuksel H: Role of nanotechnology in targeted drug delivery and imaging: A concise review. Nanomedicine. 1:193–212. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Panchapakesan B and Wickstrom E: Nanotechnology for sensing, imaging, and treating cancer. Surg Oncol Clin N Am. 16:293–305. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li XY, Xu L, Li HL, Du J, Liu XS and Li FH: Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study. Mol Med Rep 17: 3763-3768, 2018.
APA
Li, X., Xu, L., Li, H., Du, J., Liu, X., & Li, F. (2018). Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study. Molecular Medicine Reports, 17, 3763-3768. https://doi.org/10.3892/mmr.2017.8351
MLA
Li, X., Xu, L., Li, H., Du, J., Liu, X., Li, F."Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study". Molecular Medicine Reports 17.3 (2018): 3763-3768.
Chicago
Li, X., Xu, L., Li, H., Du, J., Liu, X., Li, F."Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study". Molecular Medicine Reports 17, no. 3 (2018): 3763-3768. https://doi.org/10.3892/mmr.2017.8351
Copy and paste a formatted citation
x
Spandidos Publications style
Li XY, Xu L, Li HL, Du J, Liu XS and Li FH: Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study. Mol Med Rep 17: 3763-3768, 2018.
APA
Li, X., Xu, L., Li, H., Du, J., Liu, X., & Li, F. (2018). Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study. Molecular Medicine Reports, 17, 3763-3768. https://doi.org/10.3892/mmr.2017.8351
MLA
Li, X., Xu, L., Li, H., Du, J., Liu, X., Li, F."Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study". Molecular Medicine Reports 17.3 (2018): 3763-3768.
Chicago
Li, X., Xu, L., Li, H., Du, J., Liu, X., Li, F."Au-poly(lactic-co-glycolic) acid complex nanoparticles as ultrasound contrast agents: Preparation, characterization and in vitro study". Molecular Medicine Reports 17, no. 3 (2018): 3763-3768. https://doi.org/10.3892/mmr.2017.8351
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team