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Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression

  • Authors:
    • Yan Wang
    • Guoxing Wang
    • Lijian Cui
    • Ruixia Liu
    • Hongli Xiao
    • Chenghong Yin
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Beijing Friendship Hospital, Beijing 100050, P.R. China, Department of Emergency, Beijing Chao‑Yang Hospital, Beijing 100020, P.R. China, Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3511-3518
    |
    Published online on: December 27, 2017
       https://doi.org/10.3892/mmr.2017.8354
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Abstract

The present study aimed to investigate the effects of angiotensin (Ang) 1-7 on caerulein (CAE)-stimulated nuclear factor (NF)‑κB, Toll‑like receptor (TLR4) and cytokine expression using pancreatic acinar AR42J cells. AR42J cells were treated with 10 nmol/l CAE for various durations. In addition, cells were pretreated with various concentrations of Ang 1‑7 or A779, a specific antagonist of Ang 1‑7, and were stimulated with CAE for 12 h. Control cells were treated with vehicle (F‑12K complete medium with 2% fetal bovine serum, 10 U/ml penicillin and 100 mg/ml streptomycin) alone. The mRNA and protein expression levels of TLR4, NF‑κB, interleukin (IL)‑6, IL‑8, IL‑10 and tumor necrosis factor‑α (TNF‑α) were determined by western blotting, immunofluorescence and reverse transcription‑quantitative polymerase chain reaction. CAE treatment stimulated TLR4 and NF‑κB expression within AR42J cells. Immunofluorescence indicated that TLR4 was expressed on the membranes and in the cytoplasm of AR42J cells, whereas NF‑κB expression accumulated in the cytoplasm and nuclei. CAE‑induced expression of TLR4 and NF‑κB within AR42J cells was abrogated by 10‑5 mmol/l Ang 1‑7; however, TLR4 and NF‑κB expression was enhanced with the addition of A779, particularly 10‑5 mmol/l. In addition, treatment with 10‑6 and 10‑5 mmol/l Ang 1‑7 significantly mitigated CAE‑induced expression of IL‑6, IL‑8 and TNF‑α, whereas it enhanced IL‑10 expression. Conversely, A779 treatment enhanced the CAE‑induced expression of IL‑6, IL‑8 and TNF‑α, and reduced IL‑10 expression in AR42J cells. In conclusion, these results suggested that Ang 1‑7 may attenuate CAE‑induced inflammation by downregulating TLR4, NF‑κB and proinflammatory cytokine expression within AR42J cells. Therefore, Ang 1‑7 may exert protective effects against the pathological progression of AP in a cell model of AP induced by CAE and may be considered in the development of treatments for this disease.
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1 

Liu R, Qi H, Wang J, Wang Y, Cui L, Wen Y and Yin C: Angiotensin-converting enzyme (ACE and ACE2) imbalance correlates with the severity of cerulein-induced acute pancreatitis in mice. Exp Physiol. 99:651–663. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Wang Y, Wang J, Liu R, Qi H, Wen Y, Sun F and Yin C: Severe acute pancreatitis is associated with upregulation of the ACE2-angiotensin-(1–7)-Mas axis and promotes increased circulating angiotensin-(1–7). Pancreatology. 12:451–457. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Mori J, Patel VB, Ramprasath T, Alrob OA, DesAulniers J, Scholey JW, Lopaschuk GD and Oudit GY: Angiotensin 1–7 mediates renoprotection against diabetic nephropathy by reducing oxidative stress, inflammation, and lipotoxicity. Am J Physiol Renal Physiol. 306:F812–F821. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Lu CL, Wang Y, Yuan L, Li Y and Li XY: The angiotensin-converting enzyme 2/angiotensin (1–7)/Mas axis protects the function of pancreatic β cells by improving the function of islet microvascular endothelial cells. Int J Mol Med. 34:1293–300. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Yuan L, Lu CL, Wang Y, Li Y and Li XY: Ang (1–7) protects islet endothelial cells from palmitate-induced apoptosis by AKT, eNOS, p38 MAPK, and JNK pathways. J Diabetes Res. 2014:3914762014. View Article : Google Scholar : PubMed/NCBI

6 

Wang J, Liu R, Qi H, Wang Y, Cui L, Wen Y, Li H and Yin C: The ACE2-angiotensin-(1–7)-Mas axis protects against pancreatic cell damage in cell culture. Pancreas. 44:266–272. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Gordon S: Pattern recognition receptors: Doubling up for the innate immune response. Cell. 111:927–930. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Pan LF, Yu L, Wang LM, He JT, Sun JL, Wang XB, Bai ZH, Wang H, Yan TL and Pei HH: The Toll-like receptor 4 antagonist TAK-242 protects against chronic pancreatitis in rats. Mol Med Rep. 16:3863–3868. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Li G, Wu X, Yang L, He Y, Liu Y, Jin X and Yuan H: [Corrigendum] TLR4-mediated NF-κB signaling pathway mediates HMGB1-induced pancreatic injury in mice with severe acute pancreatitis. Int J Mol Med. 38:13132016. View Article : Google Scholar : PubMed/NCBI

10 

Awla D, Abdulla A, Regnér S and Thorlacius H: TLR4 but not TLR2 regulates inflammation and tissue damage in acute pancreatitis induced by retrograde infusion of taurocholate. Inflamm Res. 60:1093–1098. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Xue J and Habtezion A: Carbon monoxide-based therapy ameliorates acute pancreatitis via TLR4 inhibition. J Clin Invest. 124:437–447. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Li S, Lu H, Hu X, Chen W, Xu Y and Wang J: Expression of TLR4-MyD88 and NF-κB in the iris during endotoxin-induced uveitis. Mediators Inflamm. 2010:7482182010. View Article : Google Scholar : PubMed/NCBI

13 

Chan YC and Leung PS: Angiotensin II type 1 receptor-dependent nuclear factor-kappaB activation-mediated proinflammatory actions in a rat model of obstructive acute pancreatitis. J Pharmacol Exp Ther. 323:10–18. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Yu JH, Lim JW and Kim H: Altered gene expression in cerulein-stimulated pancreatic acinar cells: Pathologic mechanism of acute pancreatitis. Korean J Physiol Pharmacol. 13:409–416. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Furukawa H, Shinmura A, Tajima H, Tsukada T, Nakanuma S, Okamoto K, Sakai S, Makino I, Nakamura K, Hayashi H, et al: Concentration of tissue angiotensin II increases with severity of experimental pancreatitis. Mol Med Rep. 8:335–338. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Shimizu K: Mechanisms of pancreatic fibrosis and applications to the treatment of chronic pancreatitis. J Gastroenterol. 43:823–832. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Dange RB, Agarwal D, Masson GS, Vila J, Wilson B, Nair A and Francis J: Central blockade of TLR4 improves cardiac function and attenuates myocardial inflammation in angiotensin II-induced hypertension. Cardiovasc Res. 103:17–27. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Lv J, Chen Q, Shao Y, Chen Y and Shi J: Cross-talk between angiotensin-II and toll-like receptor 4 triggers a synergetic inflammatory response in rat mesangial cells under high glucose conditions. Biochem Biophys Res Commun. 459:264–269. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Wolf G, Bohlender J, Bondeva T, Roger T, Thaiss F and Wenzel UO: Angiotensin II upregulates toll-like receptor 4 on mesangial cells. J Am Soc Nephrol. 17:1585–1593. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Santos SH, Andrade JM, Fernandes LR, Sinisterra RD, Sousa FB, Feltenberger JD, Alvarez-Leite JI and Santos RA: Oral Angiotensin-(1–7) prevented obesity and hepatic inflammation by inhibition of resistin/TLR4/MAPK/NF-κB in rats fed with high-fat diet. Peptides. 46:47–52. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Ju KD, Lim JW, Kim KH and Kim H: Potential role of NADPH oxidase-mediated activation of Jak2/Stat3 and mitogen-activated protein kinases and expression of TGF-β1 in the pathophysiology of acute pancreatitis. Inflamm Res. 60:791–800. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Johnson GB, Brunn GJ and Platt JL: Cutting edge: An endogenous pathway to systemic inflammatory response syndrome (SIRS)-like reactions through Toll-like receptor 4. J Immunol. 172:20–24. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Lai JL, Liu YH, Liu C, Qi MP, Liu RN, Zhu XF, Zhou QG, Chen YY, Guo AZ and Hu CM: Indirubin inhibits LPS-induced inflammation via TLR4 abrogation mediated by the NF-κB and MAPK signaling pathways. Inflammation. 40:1–12. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Xu M, Wang KN, Wu K and Wang XP: Pyrrolidine dithiocarbamate inhibits nuclear factor κB and Toll-like receptor 4 expression in rats with acute necrotizing pancreatitis. Gut Liver. 9:411–416. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Simões e Silva AC, Silveira KD, Ferreira AJ and Teixeira MM: ACE2, angiotensin-(1–7) and Mas receptor axis in inflammation and fibrosis. Br J Pharmacol. 169:477–492. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Aoun E, Chen J, Reighard D, Gleeson FC, Whitcomb DC and Papachristou GI: Diagnostic accuracy of interleukin-6 and interleukin-8 in predicting severe acute pancreatitis: A meta-analysis. Pancreatology. 9:777–785. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang G, Cui L, Liu R, Xiao H and Yin C: Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression. Mol Med Rep 17: 3511-3518, 2018.
APA
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., & Yin, C. (2018). Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression. Molecular Medicine Reports, 17, 3511-3518. https://doi.org/10.3892/mmr.2017.8354
MLA
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., Yin, C."Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression". Molecular Medicine Reports 17.3 (2018): 3511-3518.
Chicago
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., Yin, C."Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression". Molecular Medicine Reports 17, no. 3 (2018): 3511-3518. https://doi.org/10.3892/mmr.2017.8354
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang G, Cui L, Liu R, Xiao H and Yin C: Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression. Mol Med Rep 17: 3511-3518, 2018.
APA
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., & Yin, C. (2018). Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression. Molecular Medicine Reports, 17, 3511-3518. https://doi.org/10.3892/mmr.2017.8354
MLA
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., Yin, C."Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression". Molecular Medicine Reports 17.3 (2018): 3511-3518.
Chicago
Wang, Y., Wang, G., Cui, L., Liu, R., Xiao, H., Yin, C."Angiotensin 1-7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression". Molecular Medicine Reports 17, no. 3 (2018): 3511-3518. https://doi.org/10.3892/mmr.2017.8354
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