Using IFN-γ antibodies to identify the pathogens of fungal rhinosinusitis: A novel immunohistochemical approach

Yan,Yuyan; Zhao,Zuotao; Dong,Gehong; Han,Yiding; Yang,Dongmei; Yin,Hongyan; Piao,Yingshi; He,Chunyan; Tian,Cheng; Wan,Hongfei; Li,Xue; Jin,Yulan; Fang,Jugao; Liu,Honggang

doi:10.3892/mmr.2017.8359

March-2018 Volume 17 Issue 3

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March-2018 Volume 17 Issue 3

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Article Open Access

Using IFN-γ antibodies to identify the pathogens of fungal rhinosinusitis: A novel immunohistochemical approach

Authors:
- Yuyan Yan
- Zuotao Zhao
- Gehong Dong
- Yiding Han
- Dongmei Yang
- Hongyan Yin
- Yingshi Piao
- Chunyan He
- Cheng Tian
- Hongfei Wan
- Xue Li
- Yulan Jin
- Jugao Fang
- Honggang Liu
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Affiliations: Department of Pathology, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Dermatology, First Hospital, Peking University, Beijing 100034, P.R. China, Department of ENT, Affiliated Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China

Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3627-3632
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Published online on: December 27, 2017

https://doi.org/10.3892/mmr.2017.8359
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Abstract

Fungal rhinosinusitis (FRS) is commonly caused by various Aspergillus species (spp) and Mucorales fungi, and the treatment and prognosis of cases differ depending on the causative fungus. The present study describes a novel immunohistochemical method that has high sensitivity and specificity for distinguishing between these two types of fungi in patients with FRS. Three groups were included in the study. Group A included formalin‑fixed paraffin‑embedded blocks of 51 nasal tissue specimens of patients with FRS (27 Aspergillus spp and 24 Mucorales) that were continuously obtained from the Department of Pathology of Tongren Hospital in Beijing as the experimental group and 34 cultures (26 Aspergillus spp and 8 Mucorales) of FRS that were randomly selected from the bacterial laboratory of Tongren Hospital in Beijing to verify the staining results of the paraffin‑embedded blocks. Formalin‑fixed paraffin‑embedded blocks of 10 esophageal cancer specimens were included in Group B as the positive control group. All specimens in Groups A and B were stained with interferon‑γ (IFN‑γ) antibody. Group C consisted of the same specimens as described in Group A, however, when performing the immunohistochemical assay, IFN‑γ antibody was replaced by PBS and this served as the negative control group. The differences in IFN‑γ immunohistochemical staining between Aspergillus spp and Mucorales were analyzed. Staining of IFN‑γ in paraffin‑embedded samples was positive in 92.6% (25/27) of specimens in which Aspergillus spp were the causative pathogen, which was significantly higher compared with specimens in which Mucorales was causative (P<0.001), with only 4.2% (1/24) of specimens staining positive for IFN‑γ. Immunohistochemical staining of cell cultures was 100% positive for Aspergillus spp, whereas all Mucorales were negative. Thus, the results of the current study indicated that IFN‑γ antibody immunohistochemical staining may be used as a novel diagnostic tool to distinguish between Aspergillus spp and Mucorales when identifying the causative agent in FRS, providing a useful supplementary test to the current immunohistochemical methods in the clinical diagnosis of FRS.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Using IFN-γ antibodies to identify the pathogens of fungal rhinosinusitis: A novel immunohistochemical approach

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