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Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury

  • Authors:
    • Runxin Li
    • Huawei Liu
    • Haitao Huang
    • Wenting Bi
    • Rongzeng Yan
    • Xinying Tan
    • Weisheng Wen
    • Chao Wang
    • Wenling Song
    • Yanhua Zhang
    • Feng Zhang
    • Min Hu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Stomatology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Stomatology, Beijing First Hospital of Integrated Chinese and Western Medicine, Beijing 100026, P.R. China, Beijing Yierkang Bioengineering Development Center, Beijing 102699, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4360-4368
    |
    Published online on: January 8, 2018
       https://doi.org/10.3892/mmr.2018.8388
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Abstract

In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each group), in which the nerve was either not treated (control group) or treated with chitosan conduit, hyaluronic acid, or chitosan conduit coupled with hyaluronic acid following clamp injury to the sciatic nerve. The surgical sites were evaluated by assessing the sciatic functional index, the degree of scar adhesions, the numbers of myelinated nerve fibers, the average diameter of myelinated nerve fibers and the myelin sheath thickness. Larger epineurial scar thickness was observed in the control groups compared with the treatment groups at 4, 8 and 12 weeks following surgery. There was no significant difference in scar adhesion among the four groups at 4 weeks following surgery. However, animals receiving chitosan coupled with HA demonstrated better neural recovery, as measured by reduced nerve adherence to surrounding tissues, less scar adhesion, increased number of axons, nerve fiber diameter and myelin thickness. In conclusion, the application of chitosan conduit combined with HA, to a certain extent, inhibited sciatic nerve extraneural scaring and adhesion, and promoted neural regeneration and recovery.
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Copy and paste a formatted citation
Spandidos Publications style
Li R, Liu H, Huang H, Bi W, Yan R, Tan X, Wen W, Wang C, Song W, Zhang Y, Zhang Y, et al: Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury. Mol Med Rep 17: 4360-4368, 2018.
APA
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X. ... Hu, M. (2018). Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury. Molecular Medicine Reports, 17, 4360-4368. https://doi.org/10.3892/mmr.2018.8388
MLA
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X., Wen, W., Wang, C., Song, W., Zhang, Y., Zhang, F., Hu, M."Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury". Molecular Medicine Reports 17.3 (2018): 4360-4368.
Chicago
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X., Wen, W., Wang, C., Song, W., Zhang, Y., Zhang, F., Hu, M."Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury". Molecular Medicine Reports 17, no. 3 (2018): 4360-4368. https://doi.org/10.3892/mmr.2018.8388
Copy and paste a formatted citation
x
Spandidos Publications style
Li R, Liu H, Huang H, Bi W, Yan R, Tan X, Wen W, Wang C, Song W, Zhang Y, Zhang Y, et al: Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury. Mol Med Rep 17: 4360-4368, 2018.
APA
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X. ... Hu, M. (2018). Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury. Molecular Medicine Reports, 17, 4360-4368. https://doi.org/10.3892/mmr.2018.8388
MLA
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X., Wen, W., Wang, C., Song, W., Zhang, Y., Zhang, F., Hu, M."Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury". Molecular Medicine Reports 17.3 (2018): 4360-4368.
Chicago
Li, R., Liu, H., Huang, H., Bi, W., Yan, R., Tan, X., Wen, W., Wang, C., Song, W., Zhang, Y., Zhang, F., Hu, M."Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury". Molecular Medicine Reports 17, no. 3 (2018): 4360-4368. https://doi.org/10.3892/mmr.2018.8388
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