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Article

miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1

  • Authors:
    • Qiliang Liu
    • Yong Guo
    • Yang Wang
    • Xianqiong Zou
    • Zhixiong Yan
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Engineering, College of Biotechnology of Guilin Medical University, Guilin, Guangxi 541004, P.R. China
  • Pages: 4797-4802
    |
    Published online on: January 9, 2018
       https://doi.org/10.3892/mmr.2018.8416
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Abstract

Casein kinase 2-interacting protein 1 (CKIP-1) is a negative regulator for bone formation. Previously, using bioinformatics analysis, CKIP‑1 has been predicted to serve the role of target gene of miR‑98‑5p. In the present study, the potential role of miR‑98‑5p in regulating osteoblast differentiation through CKIP‑1 was investigated. Following pre‑treatment with microRNA (miR)‑98‑5p agomir or miR‑98‑5p antagomir, MC3T3‑E1 cells were cultured in an osteoinductive medium. Subsequently, the expression of miR‑98‑5p, CKIP‑1 and levels of osteoblast differentiation markers, including alkaline phosphatase, matrix mineralization, osteocaicin, collagen type I, runt‑related transcription factor 2 and osteopontin were assayed. Using a dual‑luciferase reporter assay, it was demonstrated that CKIP‑1 was the target gene of miR‑98‑5p. miR‑98‑5p was upregulated as a result of treatment with miR‑98‑5p agomir and promoted osteoblast differentiation. Conversely, miR‑98‑5p antagomir inhibited miR‑98‑5p expression and osteoblast differentiation. miR‑98‑5p targeted CKIP‑1 by binding to its 3'‑untranslated region. Furthermore, miR‑98‑5p overexpression decreased the protein levels of CKIP‑1 and inhibition of miR‑98‑5p increased the protein levels of CKIP‑1. The results of the present study indicated that CKIP‑1 was a target gene of miR‑98‑5p and that miR‑98‑5p regulated osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Q, Guo Y, Wang Y, Zou X and Yan Z: miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1. Mol Med Rep 17: 4797-4802, 2018.
APA
Liu, Q., Guo, Y., Wang, Y., Zou, X., & Yan, Z. (2018). miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1. Molecular Medicine Reports, 17, 4797-4802. https://doi.org/10.3892/mmr.2018.8416
MLA
Liu, Q., Guo, Y., Wang, Y., Zou, X., Yan, Z."miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1". Molecular Medicine Reports 17.3 (2018): 4797-4802.
Chicago
Liu, Q., Guo, Y., Wang, Y., Zou, X., Yan, Z."miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1". Molecular Medicine Reports 17, no. 3 (2018): 4797-4802. https://doi.org/10.3892/mmr.2018.8416
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Q, Guo Y, Wang Y, Zou X and Yan Z: miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1. Mol Med Rep 17: 4797-4802, 2018.
APA
Liu, Q., Guo, Y., Wang, Y., Zou, X., & Yan, Z. (2018). miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1. Molecular Medicine Reports, 17, 4797-4802. https://doi.org/10.3892/mmr.2018.8416
MLA
Liu, Q., Guo, Y., Wang, Y., Zou, X., Yan, Z."miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1". Molecular Medicine Reports 17.3 (2018): 4797-4802.
Chicago
Liu, Q., Guo, Y., Wang, Y., Zou, X., Yan, Z."miR‑98‑5p promotes osteoblast differentiation in MC3T3‑E1 cells by targeting CKIP‑1". Molecular Medicine Reports 17, no. 3 (2018): 4797-4802. https://doi.org/10.3892/mmr.2018.8416
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