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Article

Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma

  • Authors:
    • Yuan Xu
    • Mei‑Rong Zhu
    • Jing‑Yong Zhang
    • Guo‑Min Si
    • Jia‑Ju Lv
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, P.R. China, Department of Urology, Jinan Central Hospital, Shandong University, Jinan, Shandong 250013, P.R. China
  • Pages: 4729-4735
    |
    Published online on: January 11, 2018
       https://doi.org/10.3892/mmr.2018.8421
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Abstract

Ubiquitin specific peptidase 39 (USP39) serves important roles in mRNA processing and is involved in tumorigenesis of multiple solid malignancies. However, the influence and underlying mechanism of USP39 on human renal cell carcinomas (RCC) remain to be elucidated. The current study investigated the functional roles of USP39 in human RCC cell lines. siRNA‑mediated RNA interference was used to downregulate USP39 in RCC cells. CCK‑8, wound healing and invasion assays were performed to assess the proliferative ability and metastatic potential. The cell cycle distribution and apoptosis were evaluated by flow cytometry. The activity of signaling pathways and the expression of cell cycle‑related proteins were detected by western blot analysis. The siRNA‑directed RNA interference targeting USP39 could effectively downregulate the expression level of USP39 in two RCC cell lines. Depletion of USP39 by siRNA significantly suppressed cell growth and decreased invasive capacity of RCC cells. Silencing of USP39 induced cell apoptosis and cell cycle arrest at G2/M phase. Additionally, the expression levels of apoptotic and G2/M phase‑related proteins were notably decreased following depletion of USP39. Mechanistically, downregulation of USP39 blocked the activation of Akt and extracellular signal regulated kinase signaling pathways in RCC cells. These findings indicate that USP39 may serve as an oncogenic factor in RCC and could be a potential therapeutic candidate for human RCCs.
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1 

Rini BI, Campbell SC and Escudier B: Renal cell carcinoma. Lancet. 373:1119–1132. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Leibovich BC, Lohse CM, Crispen PL, Boorjian SA, Thompson RH, Blute ML and Cheville JC: Histological subtype is an independent predictor of outcome for patients with renal cell carcinoma. J Urol. 183:1309–1315. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Tyritzis SI, Papadoukakis S, Katafigiotis I, Adamakis I, Anastasiou I, Stravodimos KG, Alamanis C, Mitropoulos D and Constantinides CA: Implementation and external validation of Preoperative Aspects and Dimensions Used for an Anatomical (PADUA) score for predicting complications in 74 consecutive partial nephrectomies. BJU Int. 109:1813–1818. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Ljungberg B, Campbell SC, Choi HY, Jacqmin D, Lee JE, Weikert S and Kiemeney LA: The epidemiology of renal cell carcinoma. Eur Urol. 60:615–621. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Cohen DD, Matin SF, Steinberg JR, Zagone R and Wood CG: Evaluation of the intact specimen after laparoscopic radical nephrectomy for clinically localized renal cell carcinoma identifies a subset of patients at increased risk for recurrence. J Urol. 173:1487–1491. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Escudier B, Albiges L and Sonpavde G: Optimal management of metastatic renal cell carcinoma: Current status. Drugs. 73:427–438. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Valadkhan S: The spliceosome: Caught in a web of shifting interactions. Curr Opin Struct Biol. 17:310–315. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Liu S and Cheng C: Alternative RNA splicing and cancer. Wiley interdisciplinary reviews RNA. 4:547–566. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Srebrow A and Kornblihtt AR: The connection between splicing and cancer. J Cell Sci. 119:2635–2641. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Lygerou Z, Christophides G and Séraphin B: A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. Mol Cell Biol. 19:2008–2020. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Makarova OV, Makarov EM and Lührmann R: The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes. EMBO J. 20:2553–2563. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Clague MJ, Barsukov I, Coulson JM, Liu H, Rigden DJ and Urbé S: Deubiquitylases from genes to organism. Physiol Rev. 93:1289–1315. 2013. View Article : Google Scholar : PubMed/NCBI

13 

van Leuken RJ, Luna-Vargas MP, Sixma TK, Wolthuis RM and Medema RH: Usp39 is essential for mitotic spindle checkpoint integrity and controls mRNA-levels of aurora B. Cell Cycle. 7:2710–2719. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Wang H, Ji X, Liu X, Yao R, Chi J, Liu S, Wang Y, Cao W and Zhou Q: Lentivirus-mediated inhibition of USP39 suppresses the growth of breast cancer cells in vitro. Oncol Rep. 30:2871–2877. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Yuan X, Sun X, Shi X, Jiang C, Yu D, Zhang W, Guan W, Zhou J, Wu Y, Qiu Y and Ding Y: USP39 promotes the growth of human hepatocellular carcinoma in vitro and in vivo. Oncol Rep. 34:823–832. 2015. View Article : Google Scholar : PubMed/NCBI

16 

An Y, Yang S, Guo K, Ma B and Wang Y: Reduced USP39 expression inhibits malignant proliferation of medullary thyroid carcinoma in vitro. World J Surg Oncol. 13:2552015. View Article : Google Scholar : PubMed/NCBI

17 

Li KY, Zhang J, Jiang LC, Zhang B, Xia CP, Xu K, Chen HY, Yang QZ, Liu SW and Zhu H: Knockdown of USP39 by lentivirus-mediated RNA interference suppresses the growth of oral squamous cell carcinoma. Cancer Biomark. 16:137–144. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Huang Y, Pan XW, Li L, Chen L, Liu X, Lu JL, Zhu XM, Huang H, Yang QW, Ye JQ, et al: Overexpression of USP39 predicts poor prognosis and promotes tumorigenesis of prostate cancer via promoting EGFR mRNA maturation and transcription elongation. Oncotarget. 7:22016–22030. 2016.PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Method. 25:402–408. 2001. View Article : Google Scholar

20 

Zhang JW, Zhang SS, Song JR, Sun K, Zong C, Zhao QD, Liu WT, Li R, Wu MC and Wei LX: Autophagy inhibition switches low-dose camptothecin-induced premature senescence to apoptosis in human colorectal cancer cells. Biochem Pharmacol. 90:265–275. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Yajima I, Kumasaka MY, Thang ND, Goto Y, Takeda K, Yamanoshita O, Iida M, Ohgami N, Tamura H, Kawamoto Y and Kato M: RAS/RAF/MEK/ERK and PI3K/PTEN/Akt signaling in malignant melanoma progression and therapy. Dermatol Res Pract. 2012:3541912012. View Article : Google Scholar : PubMed/NCBI

22 

Vakkalanka BK and Rini BI: Targeted therapy in renal cell carcinoma. Curr Opin Urol. 18:481–487. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Keefe SM, Nathanson KL and Rathmell WK: The molecular biology of renal cell carcinoma. Semin Oncol. 40:421–428. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Mellado B and Gascón P: Molecular biology of renal cell carcinoma. Clin Transl Oncol. 8:706–710. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Nigg EA: Cyclin-dependent protein kinases: Key regulators of the eukaryotic cell cycle. BioEssays. 17:471–480. 1995. View Article : Google Scholar : PubMed/NCBI

26 

Sartor H, Ehlert F, Grzeschik KH, Müller R and Adolph S: Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics. 13:911–912. 1992. View Article : Google Scholar : PubMed/NCBI

27 

Baldin V, Lukas J, Marcote MJ, Pagano M and Draetta G: Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 7:812–821. 1993. View Article : Google Scholar : PubMed/NCBI

28 

Hwang HC and Clurman BE: Cyclin E in normal and neoplastic cell cycles. Oncogene. 24:2776–2786. 2005. View Article : Google Scholar : PubMed/NCBI

29 

O'Rourke MG and Ellem KA: John Kerr and apoptosis. Med J Aust. 173:616–617. 2000.PubMed/NCBI

30 

Rathore S, Datta G, Kaur I, Malhotra P and Mohmmed A: Disruption of cellular homeostasis induces organelle stress and triggers apoptosis like cell-death pathways in malaria parasite. Cell Death Dis. 6:e18032015. View Article : Google Scholar : PubMed/NCBI

31 

Agarwal A, Mahfouz RZ, Sharma RK, Sarkar O, Mangrola D and Mathur PP: Potential biological role of poly (ADP-ribose) polymerase (PARP) in male gametes. Reprod Biol Endocrinol. 7:1432009. View Article : Google Scholar : PubMed/NCBI

32 

Rao VN and Reddy ES: elk-1 proteins interact with MAP kinases. Oncogene. 9:1855–1860. 1994.PubMed/NCBI

33 

Freeman-Cook KD, Autry C, Borzillo G, Gordon D, Barbacci-Tobin E, Bernardo V, Briere D, Clark T, Corbett M, Jakubczak J, et al: Design of selective, ATP-competitive inhibitors of Akt. J Med Chem. 53:4615–4622. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Zhu MR, Zhang JY, Si GM and Lv JJ: Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma. Mol Med Rep 17: 4729-4735, 2018.
APA
Xu, Y., Zhu, M., Zhang, J., Si, G., & Lv, J. (2018). Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma. Molecular Medicine Reports, 17, 4729-4735. https://doi.org/10.3892/mmr.2018.8421
MLA
Xu, Y., Zhu, M., Zhang, J., Si, G., Lv, J."Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma". Molecular Medicine Reports 17.3 (2018): 4729-4735.
Chicago
Xu, Y., Zhu, M., Zhang, J., Si, G., Lv, J."Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma". Molecular Medicine Reports 17, no. 3 (2018): 4729-4735. https://doi.org/10.3892/mmr.2018.8421
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Zhu MR, Zhang JY, Si GM and Lv JJ: Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma. Mol Med Rep 17: 4729-4735, 2018.
APA
Xu, Y., Zhu, M., Zhang, J., Si, G., & Lv, J. (2018). Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma. Molecular Medicine Reports, 17, 4729-4735. https://doi.org/10.3892/mmr.2018.8421
MLA
Xu, Y., Zhu, M., Zhang, J., Si, G., Lv, J."Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma". Molecular Medicine Reports 17.3 (2018): 4729-4735.
Chicago
Xu, Y., Zhu, M., Zhang, J., Si, G., Lv, J."Knockdown of ubiquitin‑specific peptidase 39 inhibits the malignant progression of human renal cell carcinoma". Molecular Medicine Reports 17, no. 3 (2018): 4729-4735. https://doi.org/10.3892/mmr.2018.8421
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